Use of monolithic supports for fast analysis of drugs and metabolites in plasma by direct injection
Tóm tắt
Commercial monolithic columns were evcluated as extraction supports for the analysis of various drugs and their metabolites from plasma. For this purpose, a precolumn packed with a monolithic phase was used as extraction and enrichment support and integrated in a column-switching system for the direct and simultaneous analysis of fluoxetine and norfluoxetine, methadone and EDDP, flunitrazepam and norflunitrazepom in humon plasma. In this configuration, plasma samples were directly injected onto a Chromolith Flash precolumn (25×4.6 mm I.D.) coupled to a microbore analytical column packed with a conventional reversed-phase malerial. Detection was by an electrospray-ionization mass spectrometer (ESl-MS). Total analysis time, including extraction and separation of the six analytes, was <10 min. For all the investigated compounds, the limits of quantitation (LOQ) were estimated in the ng.mL1 range for an injection volume of 50 μL. The method was also applied to a real clinical case to demonstrate its applicability.
Tài liệu tham khảo
Henion, J.; Brewer, E.; Rule, G.,Anal. Chem. 1998,70, 650A.
Klink, F.; Majors, R.E.LG-GC 2000,13, 396.
Bonfiglio, R.; King, R.C.; Olah, T.V.; Merkle, K.Rapid Commun. Mass Spectrom. 1999,13, 1175.
Pinkerton, T.C.J. Chromatogr. 1997,544, 13.
Boos, K.S.; Rudolphi, A.LC-GC 1997,15, 602.
Oertel, R.; Richter, K.; Gramatte, T.; Kirch, W.J. Chromatogr. A 1998,797, 203.
Schafer, C.; Lubda, D.J. Chromatogr. A 2001, 909, 73.
Song, D.; Au, J.L.S.,J. Chromatogr. B 1996,676, 165.
Martinez Fernandez, J.; Martinez Vidal, J.L.; Parrilla Vazquez, P.; Garrido Frenich, A.Chromatographia 2001,53, 503.
Jemal, M.; Huang, M.; Jiang, X.; Mao, Y.; Powell, M. L.Rapid Commun. Mass Spectrom. 1999,13, 2125.
Zimmer, D.; Pickard, V.; Czembor, W.; Muller, C.J. Chromatogr. A 1999,854, 23.
Ding, J.; Neue, U.D.Rapid Commun. Mass Spectrom. 1999,13, 2151.
Brignol, N., Bakhtiar, R.; Dou, L.; Majumbar, T.; Tse, F.L.S.Rapid Commun. Mass Spectrom. 2000,14, 141.
Wu, J.T.; Zeng, H.; Qian, M.; Brogdon, B.L.; Unger, S.E.Anal. Chem. 2000,72, 61.
Quin, H.M.; Takarewski, J.J.International Patent Number WO97/16724.
Herman, J.L.Rapid Commun. Mass Spectrom. 2002,16, 421.
Tanaka, N.; Nagayama, H.; Kobayashi, H.; Ikegami, T.J. High Resol. Chromatogr. 2000,23, 111.
Cabrera, K.; Lubda, D.,J. High Resol. Chromatogr. 2000,23, 93.
Regnier, F.E.J. High Resol. Chromatogr. 2000,23, 19.
Dear, G.; Plumb, R.; Mallett, D.Rapid Commun. Mass Spectrom. 2001,15, 152.
Plumb, R.; Dear, G.; Mallett, D.; Ayrton J.Rapid Commun. Mass Spectrom. 2001,15, 986.
Wu, J.T.; Zeng, H.; Deng, Y.; Unger, S.E.Rapid Commun. Mass Spectrom. 2001,15, 1113.
Kaufmann, B.; Souverain, S.; Cherkaoui, S.; Christen, P.; Veuthey, J.L.Chromatographia 2002,56, 137.
Plumb, R.; Dear, G.; Mallett, D.; Ayrton, J.Rapid Commun. Mass Spectrom. 2001,15, 986.
Ayrton, J.; Dear, G.J.; Leavens, W.J.; Mallett, D.N.; Plumb, R.S.Rapid Commun. Mass Spectrom. 1997,11, 1953.
Ayrton, J.; Clare, R.A.; Dear, G.J.; Mallett, D.N.Rapid Commun. Mass Spectrom. 1999,13, 1657.
Van Eeckhout, N.; Castro Perez, J.; Claereboudt, J.; Vandeputte R.; Van Peteghem, C.Rapid Commun. Mass Spectrom. 2000,14, 280.
Bayliss, M.K.; Little, D.; Mallett, D.N.; Plumb, R.S.Rapid Commun. Mass Spectrom. 2000,14, 2039.
Jemal, M.; Xia, Y.Q.; Whigan, D.B.Rapid Commun. Mass Spectrom. 1998,12, 1389.
Oertel, R.; Richter, K.; Gramatte, T.; Kirch, W.J. Chromatogr. A 1998,797, 203.
FDA Guidance for Industry, Bioanalytical Method Validation, May2001.
Vissers, J.P.C.; Claessens, H.A.; Cramers, C.A.J. Chromatogr. A 1997,779, 1.
Vissers, J.P.C.,J. Chromatogr. A 1999,856, 117.