Use of Thiophilic Adsorption Chromatography for the One-Step Purification of a Bacterially Produced Antibody Fab Fragment without the Need for an Affinity Tag
Tài liệu tham khảo
Skerra, 1993, Bacterial expression of immunoglobulin fragments, Curr. Opin. Immunol., 5, 256, 10.1016/0952-7915(93)90014-J
Carter, 1992, High level Escherichia coli expression and production of a bivalent humanized antibody fragment, Biotechnology, 10, 163, 10.1038/nbt0292-163
Skerra, 1988, Assembly of a functional immunoglobulin Fv fragment in Escherichia coli, Science, 240, 1038, 10.1126/science.3285470
Antony, 1992, Production of stable anti-digoxin Fv in Escherichia coli, Mol. Immunol., 29, 1237, 10.1016/0161-5890(92)90060-B
Cheadle, 1992, Cloning and expression of the variable regions of mouse myeloma protein MOPC315 in E. coli: Recovery of active Fv fragments, Mol. Immunol., 29, 21, 10.1016/0161-5890(92)90152-N
Munro, 1986, An Hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein, Cell, 46, 291, 10.1016/0092-8674(86)90746-4
Ward, 1989, Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli, Nature, 341, 544, 10.1038/341544a0
Schmidt, 1994, One-step affinity purification of bacterially produced proteins by means of the “Strep Tag” and immobilized recombinant core streptavidin, J. Chromatogr. A, 676, 337, 10.1016/0021-9673(94)80434-6
Skerra, 1994, A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments, Gene, 141, 79, 10.1016/0378-1119(94)90131-7
Essen, 1993, Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine, J. Chromatogr. A, 657, 55, 10.1016/0021-9673(93)83034-P
Skerra, 1991, The functional expression of antibody Fv fragments in Escherichia coli: Improved vectors and a generally applicable purification technique, Biotechnology, 9, 273, 10.1038/nbt0391-273
Molloy, 1995, Expression and purification strategies for the production of single-chain antibody and T-cell receptor fragments in E, coli. Mol. Biotechnol., 4, 239, 10.1007/BF02779017
Porath, 1987, “Thiophilic” interaction and the selective adsorption of proteins, Trends Biotechnol., 5, 225, 10.1016/0167-7799(87)90052-7
Porath, 1985, Thiophilic adsorption—A new method for protein fractionation, FEBS Lett., 185, 306, 10.1016/0014-5793(85)80928-5
Lihme, 1991, Thiophilic adsorption chromatography: The separation of serum proteins, Anal. Biochem., 192, 64, 10.1016/0003-2697(91)90184-U
Sulk, 1992, Application of phase partitioning and thiophilic adsorption chromatography to the purification of monoclonal antibodies from cell culture fluid, J. Immunol. Methods, 149, 165, 10.1016/0022-1759(92)90247-Q
Hutchens, 1986, Thiophilic adsorption of immunoglobulins—Analysis of conditions optimal for selective immobilization and purification, Anal. Biochem., 159, 217, 10.1016/0003-2697(86)90331-3
Caroni, 1988, Antibody against myelin-associated inhibitor of neurite growth neutralizes nonpermissive substrate properties of CNS white matter, Neuron, 1, 85, 10.1016/0896-6273(88)90212-7
Bandtlow, 1996, The Escherichia coli-derived Fab fragment of the IgM/κ antibody IN-1 recognizes and neutralizes myelin-associated inhibitors of neurite growth, Eur. J. Biochem., 241, 468, 10.1111/j.1432-1033.1996.00468.x
Schnell, 1990, Axonal regeneration in the rat spinal cord produced by an antibody against myelin-associated neurite growth inhibitors, Nature, 343, 269, 10.1038/343269a0
Spillmann, 1998, Identification and characterisation of a bovine neurite growth inhibitor (bNI-220), J. Biol. Chem., 273, 19283, 10.1074/jbc.273.30.19283
Z'Graggen, 1998, Functional recovery and enhanced corticofugal plasticity after unilateral pyramidal tract lesion and blockade of myelin-associated neurite growth inhibitors in adult rats, J. Neurosci., 18, 4744, 10.1523/JNEUROSCI.18-12-04744.1998
Yanisch-Perron, 1985, Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC vectors, Gene, 33, 103, 10.1016/0378-1119(85)90120-9
Geisselsoder, 1987, Efficient site-directed in vitro mutagenesis, BioTechniques, 5, 786
Bullock, 1987, XL1-Blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection, BioTechniques, 5, 376
Sambrook, 1989
Schiweck, 1997, Sequence analysis and bacterial production of the anti-c-myc antibody 9E10: The VH domain has an extended CDR-H3 and exhibits unusual solubility, FEBS Lett., 414, 33, 10.1016/S0014-5793(97)00983-6
Skerra, 1994, Use of the tetracycline promotor for the tightly regulated production of a murine antibody fragment in Escherichia coli, Gene, 151, 131, 10.1016/0378-1119(94)90643-2
Fling, 1986, Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris-buffer system without urea, Anal. Biochem., 155, 83, 10.1016/0003-2697(86)90228-9
Bill, 1995, Optimization of protein G chromatography for biopharmaceutical monoclonal antibodies, J. Mol. Recognit., 8, 90, 10.1002/jmr.300080116
Stevens, 1991, Bence Jones proteins: A powerful tool for the fundamental study of protein chemistry and pathophysiology, Biochemistry, 30, 6803, 10.1021/bi00242a001
Schulze, 1994, Thiophilic adsorption chromatography of recombinant single-chain antibody fragments, Anal. Biochem., 220, 212, 10.1006/abio.1994.1322
Schiweck, 1995, Fermenter production of an artificial Fab fragment, rationally designed for the antigen cystatin, and its optimized crystallisation through constant domain shuffling, Proteins Struct. Funct. Genet., 23, 561, 10.1002/prot.340230411
Thallmair, 1998, Neurite growth inhibitor restrict plasticity and functional recovery following corticospinal tract lesions, Nature Neurosci., 1, 124, 10.1038/373
Neidhardt, 1990