Use of TaqMan gene probe for real-time monitoring of acidophilic hydrogen-producing bacteria
Tóm tắt
Using a TaqMan gene probe targeting the 16S rDNA of acidophilic hydrogen-producing bacteria (HPB), the abundance of this HPB in the biomass was found to increase from 0.02 to 72% in a single batch treating rice slurry waste at pH 4.5 over 130 h. The corresponding abundances were 4.4% in the batch operated at pH 5.0 and 0.01–0.02% at pH 5.5–6.5. During the growth phase, the generation time for the acidophilic HPB at pH 4.5 averaged 3.5 h.
Tài liệu tham khảo
Abell GCJ, Bowman JP (2005) Colonization and community dynamics of class Flavobacteria on diatom detritus in experimental mesocosms based on Southern Ocean seawater. FEMS Microbiol Ecol 53:379–391
Adams MWW, Stiefel EI (1998) Biological hydrogen production: not so elementary. Science 282:1842–1843
Fang HHP, Liu H, Zhang T (2005) Phototrophic hydrogen production from acetate and butyrate in wastewater. Int J Hydrogen Energy 30:785–793
Fang HHP, Li CL, Zhang T (2006a) Acidophilic hydrogen production from rice slurry. Int J Hydrogen Energy 31:683–692
Fang HHP, Zhang T, Li CL (2006b) Characterization of Fe-hydrogenase genes diversity and hydrogen-producing population in an acidophilic sludge. J Biotechnol 126:357–364
Gelhaye E, Petitdemange H, Gay R (1993) Adhesion and growth rate of Clostridium cellulolyticum ATCC 35319 on crystalline cellulose. J Bacteriol 175:3452–3458
Genome Online Database (2007) Nikos Kyrpides http://genomesonline.org/ cited 2006 Oct 15
Ginzinger DG (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30:503–512
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
Klappenbach JA, Saxman PR, Cole JT et al (2001) rrndb: The ribosomal rna operon copy number database. Nucleic Acids Res 29:181–184
Nelson KE, Clayton RA, Gill SR et al (1999) Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima. Nature 399:323–329
Prescott LM, Harley JP, Klein DA (2002) Microbiology, 5th edn. McGraw-Hill, Boston
Sambrook J, Fritsch EE, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Ueno Y, Haruta S, Ishii M, Igarashi Y (2001) Microbial community in anaerobic hydrogen-producing microflora enriched from sludge compost. Appl Microbiol Biotechnol 57:555–562
Zhang T, Fang HHP (2006) Application of real-time polymerase chain reaction for quantification of microorganisms in environmental sample. Appl Microbiol Biotechnol 70:281–289