Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis

Capelo, 2009, Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques, Anal Chim Acta, 650, 151, 10.1016/j.aca.2009.07.034 Granvogl, 2007, Sample preparation by in-gel digestion for mass spectrometry-based proteomics, Anal Bioanal Chem, 389, 991, 10.1007/s00216-007-1451-4 Ding, 2003, Unmatched masses in peptide mass fingerprints caused by cross-contamination: an updated statistical result, Proteomics, 3, 1313, 10.1002/pmic.200300452 Thurgood, 2010, Proteomic analysis of proteins selectively associated with hydroxyapatite, brushite, and uric acid crystals precipitated from human urine, J Proteome Res, 9, 5402, 10.1021/pr1006312 Stamenkovic, 1986, Distribution of intermediate filament proteins in normal and diseased human glomeruli, Am J Pathol, 125, 465 Fleming, 1987, The distribution of cytokeratin antigens in the kidney and in renal tumours, Histopathology, 11, 157, 10.1111/j.1365-2559.1987.tb02619.x Tsugita, 2000, Proteome analysis of mouse brain: two-dimensional electrophoresis profiles of tissue proteins during the course of aging, Electrophoresis, 21, 1853, 10.1002/(SICI)1522-2683(20000501)21:9<1853::AID-ELPS1853>3.0.CO;2-Y Zhang, 2010, Glomerular proteins related to slit diaphragm and matrix adhesion in the foot processes are highly tyrosine phosphorylated in the normal rat kidney, Nephrol Dial Transplant, 25, 1785, 10.1093/ndt/gfp697 Xu, 2005, Two-dimensional electrophoretic profiling of normal human kidney: differential protein expression in glomerulus, cortex and medulla, J Electrophoresis, 49, 5, 10.2198/jelectroph.49.5 Jin, 2008, Analysis of expression and comparative profile of normal placental tissue proteins and those in preeclampsia patients using proteomic approaches, Anal Chim Acta, 629, 158, 10.1016/j.aca.2008.09.015 Griffin, 2010, Label-free, normalized quantification of complex mass spectrometry data for proteomic analysis, Nat Biotechnol, 28, 83, 10.1038/nbt.1592 Alvarado, 2010, A comparative study of in-gel digestions using microwave and pressure-accelerated technologies, J Biomol Tech, 21, 148