Uptake of bacteriophage λ DNA in Escherichia coli by electroporation: An alternative to in vitro packaging
Tóm tắt
By using a high field strength DC pulse of 15 kV/cm and a pulse duration of 5 ms for the transfection of E. coli by bacteriophage λ DNA, we obtained efficiencies of 1.1 × 106 (pfu/μg bacteriophage λ, DNA). This represents a 100-fold improvement over the traditional CaCl2/heat shock method and is a viable alternative to the more costly in vitro packaging of recombinant bacteriophage λ DNA for the production of cDNA and genomic libraries.
Tài liệu tham khảo
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