Up-regulation of antioxidant and glyoxalase systems by exogenous glycinebetaine and proline in mung bean confer tolerance to cadmium stress

Physiology and Molecular Biology of Plants - Tập 16 - Trang 259-272 - 2010
Mohammad Anwar Hossain1,2, Mirza Hasanuzzaman1,3, Masayuki Fujita1
1Department of Applied Biological Science, Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Kagawa, Japan
2Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh
3Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Tóm tắt

The present study investigates the possible mediatory role of exogenously applied glycinebetaine (betaine) and proline on reactive oxygen species (ROS) and methylglyoxal (MG) detoxification systems in mung bean seedlings subjected to cadmium (Cd) stress (1 mM CdCl2, 48 h). Cadmium stress caused a significant increase in glutathione (GSH) and glutathione disulfide (GSSG) content, while the ascorbate (AsA) content decreased significantly with a sharp increase in hydrogen peroxide (H2O2) and lipid peroxidation level (MDA). Ascorbate peroxidase (APX), glutathione S-transferase (GST), glutathione peroxidase (GPX), and glyoxalase I (Gly I) activities were increased in response to Cd stress, while the activities of catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and glyoxalase II (Gly II) were sharply decreased. Exogenous application of 5 mM betaine or 5 mM proline resulted in an increase in GSH and AsA content, maintenance of a high GSH/GSSG ratio and increased the activities of APX, DHAR, MDHAR, GR, GST, GPX, CAT, Gly I and Gly II involved in ROS and MG detoxification system as compared to the control and mostly also Cd-stressed plants, with a concomitant decrease in GSSG content, H2O2 and lipid peroxidation level. These findings together with our earlier findings suggest that both betaine and proline provide a protective action against Cd-induced oxidative stress by reducing H2O2 and lipid peroxidation levels and by increasing the antioxidant defense and MG detoxification systems.

Tài liệu tham khảo

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