Ultrastructural and functional changes in pancreatic acinar cells during autolysis
Tóm tắt
Effects of anoxemic cell injury on rat pancreatic acinar cells were studied in a preparation where tissue samples were incubated at temperature between 18–20° C in a moist atmosphere for 0, 0.5, 1, 3, 6, 12, and 24 h in vitro. Electron microscopy revealed that disintegration of acinar cells began by swelling of various cell compartments and gradual breakdown of cell membranes. Zymogen granules remained morphologically intact for at least 3 h. There were no signs of increased autophagic activity during the period of observation. Myelin figures and other membranous remnants of disintegrated cells, together with individual cells and cell organelles whose morphology was relatively well preserved were seen even after 24 h incubation. The secretory response of acinar cells to pancreozymin stimulation, as measured by amylase release into the incubation medium in vitro, decreased progressively closer to zero during 12 h autolysis. No active trypsin could be detected in the tissue samples during the 24 h observation time. It was concluded that during hypoxic autolysis at room temperature between 18–20° C in vitro:
Tài liệu tham khảo
Arstila, A.U., Hirsimäki, P., Trump, B.F.: Studies on the subcellular pathophysiology of sublethal chronic injury. Beitr. Path. Bd.152, 211–242 (1974)
Baggenstoss, A.H.: Pathology of pancreatitis ed. E.E. Gambill, P. 186: In: Pancreatitis. Sti Louis: C.V. Mosby Co. 1973
Becker, V.: Bauchspeicheldrüse (Inselapparat ausgenommen). In: Spezielle Pathologische Anatomie. Band 6 p. 139. Editors: Doerr, W., Seifert, G., Uehlinger, E., Berlin, Heidelberg, New York: Springer 1973
Ceska, M., Hultman, E., Ingelman, B.-A.: A new method for determination of α-amylase. Experientia (Basel)25, 555–556 (1976)
Churgy, J.-J.: Influence du mode de fixation sur la possibilité d’observer des structures myéliniques dans les hépatocytes d’embryons de poulet. J. Microscopie7, 63–80 (1968)
Collan, Y., Salmenperä, M.: Electron microscopy of post-mrotem autolysis of rat muscle tissue. Acta neuropath. (Berl.)35, 219–233 (1976)
Confer, D.B., Stenger, R.F.: The evolution of lysosomes in hypoxic liver parenchyma as seen with the electronmicroscope. Amer. J. Pathol.45, 533–546 (1964)
Eriksson, S.: Studies on α-antitrypsin deficiency. Acta. med. Scand.177, suppl. 432, 1–85 (1965)
Fahimi, H.D.: Perfusion and immersion fixation of rat liver with glutaraldehyde. Lab. Invest.16, 736–750 (1967)
Gambill, E.E.: Pancreatitis. St. Louis: C.V. Mosby Co 1973
Garcia, J.H., Kalimo, H., Kamiyo, Y, Lesser, M.V., Trump, B.F.: Comparison between regional cerebral ischemia and total cerebral ischemia. An ultrastructural study on the cat. Proc. Electron & Micr. Soc. Amer.31, 656–657 (1973)
Glinsmann, V.H., Ericksson, J.K.E.: Observation on the subcellular organisation of hepatic parencymal cells. II Evolution on reversible alteration induced by hypoxia. Lab. Invest.15, 762–777 (1966)
Herdson, P.B., Kaltenbach, J.B., Jennings, R.B.: Fine structural and biochemical changes in dog myocardium during autolysis. Amer. J. Path.57, 539–558, (1969)
Holtzer, R.L., Van Lancker, J.L.: Early changes in pancreas autolysis. Am. J. Pathol.40, 331–336 (1962)
Janigan, D.T., Nevalainen, T.J., Mac Aulay, M.A., Vethamany, V.G.: Foreign serum-induced pancreatitis in mice. I A new model of acute pancreatitis. Lab. Invest.33, 591–607 (1975)
Jones, R.J., Garcia, J.H., Mergner, W.J., Pendergrass, R.E., Valigorsky, J.M., Trump, B.F.: Effects of shock on the pancreatic acinar cell. Cellular and subecellular effects in humans. Arch. Pathol.99, 634–644 (1975)
Jones, R.T., Trump, B.F.: Cellular and subcellular effects of ischemia on the pancreatic acinar cell. In vitro studies of rat tissue. Virchows Arch. B Cell Path.19, 325–336 (1975)
Karásek, J.: Ultrastructural nuclear changes of extranucleolar ribonucleoprotein structures during autolysis of normal liver cells. Virchows Arch B Cell Path.18, 337–346 (1975)
Laiho, K.U., Shelburne, J.D., Trump, B.F.: Observations on cell volume, ultrastructure, mitochondrial conformation and vital dye uptake in Erhlich ascites tumor cells: effects of inhibiting energy production function of the plasma membrane. Amer. J. Pathol.65, 203–230 (1971)
Le Beux, Y., Hetenyi, G., Phillips, M.J.: Mitochondrial myelin-like figures: a non-spezific reactive process of mitochondrial phospholipid membranes to several stimuli. Z. Zeilforsch.99, 491–506 (1969)
Lewis, M.R.: Reversible gelation in living cells. Bull. Hopkins. Hosp.34, 373–379 (1923)
Lombardi, B., Estes, L.W., Longnecker, D.S.: Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline deficient diet. Amer. J. Pathol.79, 465–480 (1975)
Luft, J.H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961)
Majno, G., La Gattuta, M., Thomspon, T.E.: Cellular death and necrosis: chemical, physical and morphological changes in rat liver, Virchows Arch. path. Anat.33, 412–415 (1960)
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Phys.32, 1637 (1961)
Morava, A.P., Han, S.S.: Studies on hypoxia: Ultrastructural and biochemical effects of prolonged exposure on rat parotid glands. Exp. molec. Path.21, 268–287 (1974)
Nevalainen, T.J.: Effects of pilocarpine stimulation on rat pancreatic acinar cells. An electron microscopic study with morphometric analysis. Acta pathol. microbiol. Scand. sect. A, suppl.219, 1–43 (1970)
Nevalainen, T.J.: Cytotoxicity of vinblastine and vincristine to pancreatic acinar cells. Virchows Arch. B Bell Path.18, 119–127 (1975)
Nevalainen, T.J., Janigan, D.T.: Pilocarpine stimulation of exocrine pancreas secretion in vitro. Res. exp. Med.162, 161–167 (1974)
Ohisson, K., Eddeland, A.: Release of proteolytic enzymes in bileinduced pancreatitis in dogs. Gastroenterology69, 668–675 (1975)
Oudea, P.R.: Anoxic changes of liver cells. Lab. Invest.12, 486–494 (1963)
Penttilä, A., Ahonen, A.: Electron microscopical and enzyme histochemical changes in the rat myocardium during prolonged autolysis. Beitr. Path. Bd.157, 126–141 (1976)
Réz, G., Kiss, A., Bucsek, M.J., Kovács, J.: Attachement of ribosomes to endoplasmic membranes in mouse pancreas. Degranulation in vivo caused by the inducers of autophagocytosis neutral red, vinblastine, puromycin and cadmium ions, and prevented by cycloheximide. Chem.-Biol. Interaction13, 77–87 (1976)
Riede, U.N., Lobingen, A., Grünholz, P., Steimer, R., Sandritter, W.: Einfluss einer einstündigen Autolyse auf die quantitative Zytoarchitektur der Rattenleberzelle (Eine ultrastrukturellmorphometrische Studie) Beitr. Path. Bd.157, 391–411 (1976)
Rozenszajn, L.A., Epstein, Y., Fischer, D.: Myelin figures in blood cells, lysosomes and lipidoses. Am. J. Clin Path.52, 473–476 (1969)
Sabatini, D.D., Bensch, K., Barrnett, R.J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol17, 19–58 (1963)
Trump, B.F., Goldblatt, P.J., Stowell, R.E.: Studies of mouse liver in vitro. Ultrastructural alterations in the mitochondria of hepatic parenchymal cells. Lab. Invest.14, 343 (1965)
Trump, B.F., Goldblatt, P.J., Stowell, R.E.: Studies of necrosis in vitro of mouse hepatic parencymal cells. Ultrastructural and cytochemical alterations of cytosomes, cytosegresomes, multivesicular bodies and microbodies and their relation to the lysosome concept. Lab. Invest.14, 1946–1968 (1965)
Trump, B.F., Goldblatt, P. V., Stowell, R.E.: Studies of mouse liver necrosis in vitro. Ultrastructural and cytochemical alterations in hepatic parencymal cell nuclei. Lab. Invest.14, 1969–1999 (1965)
Trump, B.F., Goldblatt, P.J., Stowell, R.E.: Studies of necrosis in vitro of mouse hepatic parencymal cells. Ultrastructural alterations in endoplasmic reticulum, Golgi apparatus, plasma membrane and lipid droplets. Lab. Invest.14, 2000–2028 (1965)
Trump, B.F., Smuckler, E.A., Benditt, E.P.: A method for staining epoxy sections for light microscopy. J. Ultrastruct. Res.5, 343–348 (1961)
Venable, J.H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25, 407–408 (1961)
Walters, J.B., Israel, M.S.: General Pathology, 3 rd edition, p 85. London: Churchill 1970
Watson, M.L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. Biochem. Cytol.4, 475–479 (1958)