Ultrastructural and cytochemical studies of nucleolus-like bodies in neurons of rat sympathetic ganglia

Springer Science and Business Media LLC - Tập 93 - Trang 305-310 - 1990
A. Zareba-Kowalska1, J. A. Cidadao2, J. F. David-Ferreira2
1Laboratory of the Ultrastructure of the Central Nervous System, Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
2Department of Cell Biology, Gulbenkian Institute of Sciences, Oeiras, Portugal

Tóm tắt

Intracytoplasmic fibrillar inclusions, generally referred to as nucleolus-like bodies (NLBs) were studied by means of ultrastructural cytochemistry. The structure of these bodies was visualized by several different staining procedures: conventional electron microscopy and preferential staining methods for localization of various proteins including ribonucleoproteins, basic proteins, glycoproteins and phosphorylated proteins. The results of the cytochemical tests indicate that NLBs have an essentially proteinaceous nature. They consist of ribonucleoproteins, basic proteins and glycoproteins but do not contain phosphorylated proteins. These findings suggest that NLBs are, at least partially, of the same nature as nucleoli and coiled bodies. The origin of NLBs and their possible functional role is briefly discussed.

Tài liệu tham khảo

Ainsworth SK, Ito S, Karnovsky MJ (1972) Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodate-reactive sites. J Histochem Cytochem 20:995–1005 Anzil AP, Herrlinger H, Blinzinger K (1973) Nucleolus-like inclusions in neuronal pericarya and processes: Phase and electron microscope observations on the hypothalamus of the mouse. Z Zellforsch 146:329–337 Azevedo C (1984) Development and ultrastructural autoradiographic studies of nucleolus-like bodies (nuages) in oocytes of a viviparous teleost (Xiphophorus helleri). Cell Tissue Res 238:121–128 Courtens J-L, Loir M (1981) Ultrastructural detection of basic nucleoproteins: alcoholic phosphotungstic acid does not bind to arginine residues. J Ultrastruct Res 74:322–326 Fechner J (1986) Nucleolus-like bodies in the pineal gland of the Djungarian hamster (Phodopus sungourus). Cell Tissue Res 243:441–443 Fuge H (1976) Ultrastructure of cytoplasmic nucleolus-like bodies and nuclear RNP particles in late prophase of tipulid spermatocytes. Chromosoma 56:363–379 Grillo MA (1970) Cytoplasmic inclusions resembling nucleoli in sympathetic neurons of adult rats. J Cell Biol 45:100–117 Hindelang-Gertner C, Stoeckel ME, Porte A, Dellmann HD, Madarasz B (1974) Nematosomes or nucleolus-like bodies in hypothalamic neurons, the subfornical organ and adenohypophysial cells of the rat. Cell Tissue Res 155:211–219 Holmgren E (1899) Zur Kenntnis der Spinalganglienzellen des Kaninchens und des Frosches. Anat Anz 16:161–171 Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A Katoh Y, Shimizu N (1982) The light and electron microscopic localisation of intracytoplasmic nucleolus-like bodies in the mouse brain stained by Holmes' silver method. Arch Histol Jpn 45:325–333 Kawabata I (1965) Electron microscopy of the rat hypothalamic neurosecretory system. II. Nucleolus-like inclusion bodies in the cytoplasm of neurosecretory cells. Arch Histol Jpn 26:101–113 Kessel RG (1969) Cytodifferentiation in the Rana pipiens oocytes. I. Association between mitochondria and nucleolus-like bodies in young oocytes. J Ultrastruct Res 28:61–67 Kishi K (1972) Fine structural and cytochemical observations on cytoplasmic nucleolus-like bodies in nerve cells of rat medulla oblongata. Z Zellforsch 132:523–532 Korfsmeier KH (1970) Ultrastructurelle Veränderungen in den neurosekretorischen Zentren des Hypothalamus und in der Eminentia mediana nach Behandlung mit Cyproteronacetat (Antiandrogen). Z Zellforsch 110:600–610 Le Beux YJ (1971) An ultrastructural study of the neurosecretory cells of the medial vascular prechiasmatic gland, the preoptic recess and the anterior part of the suprachiasmatic area. I. Cytoplasmic inclusions resembling nucleoli. Z Zellforsch 114:404–440 Leduc EH, Bernhard W (1967) Recent modification of the glycol methacrylate embedding procedure. J Ultrastruct Res 19:196–199 Moyne G (1980) Methods in ultrastructural cytochemistry of the cell nucleus. Prog Histochem Cytochem 13:1–56 Norström A, Hansson HA (1973) Effects of colchicine on release of neurosecretory material from the posterior pituitary gland of the rat. Z Zellforsch 142:443–464 Peach R (1972) Nematosomes in the rat trigeminal ganglion. J Cell Biol 55:718–721 Rhode E (1896) Ganglienzellen und Neuroglia. Ein Kapitel über Vermehrung und Wachstum von Ganglienzellen. Arch Mikrosk Anat 47:121–122 Santolaya RC (1973) Nucleolus-like bodies in the neuronal cytoplasm of the mouse arcuate nucleus. Z Zellforsch 146:319–328 Shimizu N, Ishii S (1965) Electron microscopic observations on nuclear extrusion in nerve cells of the rat hypothalamus. Z Zellforsch 67:367–372 Takeuchi IK (1980) Nucleolus-like bodies in the embryonic ectodermal and mesodermal cells of postimplantation rat embryos. J Electron Microsc 29:186–189 Takeuchi IK, Takeuchi YK (1982) Ultrastructural and cytochemical studies on nucleolus-like bodies in early postimplantation rat embryos. Cell Tissue Res 226:257–266 Tanaka S, Iwasawa H, Imai K (1980) Formation of germ cell-like cells in the peritoneal epithelium of the testes of estrogentreated adult males of the Japanese red-bellied newt, Cynops pyrrhogaster pyrrhogaster. Dev Growth Differ 22:611–626 Toury R, Clérot J-C, André J (1977) Les groupements mitochondriaux des cellules germinales des poissons Téléosteén Cyprinidés. IV. Analyse biochemique des constituants du “ciment” intermitochondrial isolé. Biol Cell 30:225–232 Wartenberg H, Baumgarten HG (1969) Untersuchungen zur fluoreszenz-und electronenmikroskopischen Darstellung von 5-Hydroxytryptamin (5-HT) im Pineal-Organ von Lacerta viridis and Lacerta muralis. Z Anat Entwickl-Gesch 128:187–210 Wassef M (1979) A cytochemical study of interchromatin granules. J Ultrastruct Res 69:121–133 Wassef M, Burglen J, Bernhard W (1979) A new method for visualization of preribosomal granules in the nucleolus after acetylation. Biol Cell 34:153–158 Weakly BS (1971) Basic protein and ribonucleic acid in the cytoplasm of the ovarian oocyte in the golden hamster. Z Zellforsch 112:69–84 Worgul BV, Mandel S (1980) The formation of lens fiber terminal bodies. J Electron Microsc 29:151–157 Zareba-Kowalska A (1989) Cytochemical observations of the coiled bodies in neurons of rat sympathetic ganglia. Histochemistry 91:251–256