Ultrasound-assisted extraction of kamebakaurin from Rabdosia excisa by response surface methodology

Chemical Research in Chinese Universities - Tập 29 - Trang 1072-1077 - 2013
Jian Tang1, Rui-li Ma1, Qian-qian Li1, Chun-bao Jiang1, Rui Chen1, Zhen Ouyang1, Hai-sheng Chen2, Min Wang3
1School of Pharmacy, Jiangsu University, Zhenjiang, P.R. China
2School of Pharmacy, Second Ministry Medical University, Shanghai, P. R. China
3College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, P.R. China

Tóm tắt

For the efficient extraction of kamebakaurin(KA), the ultrasound-assisted extraction(UAE) of KA from Rabdosia excisa(R. excisa) via response surface methodology(RSM) was investigated with high-performance liquid chromatography(HPLC). Effects of the experimental parameters such as extraction solvent, ratio of liquid to plant material, extraction time and extraction temperature on the extracting efficiency of KA from R. excisa were evaluated, and the purity of KA in residual was calculated. The optimized conditions were 65.5%(volume fraction) acetone, 35 °C, time of 24.6 min with ultrasound of 80 W/L, 40 kHz, ratio of liquid to plant material at 30:1(mL/g). The maximum yield of KA is 0.708 mg/kg, with mean purity of 6.09%, indicating that ultrasound-assisted extraction is a feasible and useful method for extracting KA from R. excisa.

Tài liệu tham khảo

Editorial Board of Flora of China of Chinese Academy of Sciences, Flora of China, Science Press, Beijing, 1977, 65, 490 Yan Z. K., Li W. L., Flora of Medicinal Plants in Changbai Mountain, People’s Medical Publishing House, Beijing, 1996, 55 Gui M. Y., Aoyagi Y., Jin Y. R., Li X. W., Hasuda T., Takeya K., J. Nat. Prod., 2004, 67, 373 Sun H. D., Huang S. X., Han Q. B., Nat. Prod. Rep., 2006, 23, 673 Zhu X. H., Investigation on the Chemical and Bioactive Constituents of Isodon Excisa, East China Normal University, Shanghai, 2010 Piao Y., The Chemistry Research of Bioactive Constituents of Isodon excia in Changbai Mountain, Yanbian University, Yanbian, 2011 Lee J. H., Choi J. K., Noh M. S., Hwang B. Y., Hong Y. S., Lee J. J., Planta Med., 2004, 70, 526 Kim B. W., Koppula S., Kim I. S., Lim H. W., Hong S. M., Han S. D., Hwang B. Y., Choi D. K., J. Pharmacol. Sci., 2011, 116, 296 Hwang B. Y., Lee J. H., Koo T. H., Kim H. S., Hong Y. S., Ro J. S., Lee K. S., Lee J. J., Planta Med., 2001, 67, 406 Lee J. H., Koo T. H., Hwang B. Y., Lee J. J., J. Biolog. Chem., 2002, 277, 18411 Paris D., Patel N., Quadros A., Linan M., Bakshi P., Ait-Ghezala G., Mullan M., Neurosci. Lett., 2007, 415, 11 Zhao Q. C., Hao Y. F., Nan M. L., Li Q. J., Zhao X. J., New Preparing Method of Total Diterpenoids from Rabdosia excisa, CN201010262110.7, 2010 Wang Z. B., Zhang J., Wang S. Q., Ma H. L., Wang Q. Q., Chen X. Y., Trans. Chin. Soc. Agri. Mach., 2011, 42, 150 Vinatoru M., Ultrason. Sonochem., 2001, 8, 303 Li Y. W., Jiang G. C., Zhang C. G., Wei W. L., Chem. J. Chinese Universities, 2012, 33(1), 66 Sun H. D., Xu Y. L., Jiang B., Diterpenoids from Isodon Species, Science Press, Beijing, 2001, 19 Kim S. J., Murthy H. N., Hahn E. J., Lee H. L., Paek K. Y., Sep. Purif. Technol., 2007, 56, 401