Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis

Proceedings of the National Academy of Sciences of the United States of America - Tập 106 Số 41 - Trang 17588-17593 - 2009
Taishi Umezawa1, Naoyuki Sugiyama2, Mika Mizoguchi1,3, Shimpei Hayashi4, Fumiyoshi Myouga5, Kazuo Shinozaki3, Yasushi Ishihama2,6, Takashi Hirayama4,7, Kazuo Shinozaki1
1Gene Discovery Research Team, RIKEN Plant Science Center, 3-1-1 Kouyadai, Tsukuba, Ibaraki 305-0074, Japan.
2Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan
3Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
4International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045 Japan;
5R&D program, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan;
6PRESTO, Japan Science and Technology Agency, 5-Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan; and
7RIKEN–Advanced Science Institute, Wako, Saitama 351-0198, Japan

Tóm tắt

Abscisic acid (ABA) signaling is important for stress responses and developmental processes in plants. A subgroup of protein phosphatase 2C (group A PP2C) or SNF1-related protein kinase 2 (subclass III SnRK2) have been known as major negative or positive regulators of ABA signaling, respectively. Here, we demonstrate the physical and functional linkage between these two major signaling factors. Group A PP2Cs interacted physically with SnRK2s in various combinations, and efficiently inactivated ABA-activated SnRK2s via dephosphorylation of multiple Ser/Thr residues in the activation loop. This step was suppressed by the RCAR/PYR ABA receptors in response to ABA. However the abi1–1 mutated PP2C did not respond to the receptors and constitutively inactivated SnRK2. Our results demonstrate that group A PP2Cs act as ‘gatekeepers’ of subclass III SnRK2s, unraveling an important regulatory mechanism of ABA signaling.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 106 Số 41

17588-17593

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