Two-layer reflectometric interference spectroscopy-based immunosensing for C-reactive protein
Tóm tắt
We report on dual interference layers for use in reflectometric interference spectroscopy (RIfS)-based immunosensing. The layers consist of (a) an antibody-embedded organic/inorganic hybrid titanium dioxide (TiO2) layer (~60 nm thick) as a sensitive layer, and (b) a TiO2 layer (~110 nm thick) as an interference base layer placed on a silicone substrate by liquid phase deposition. A monoclonal antibody against C-reactive protein (anti-CRP) was co-embedded with poly(L-lysine) as an organic binder during deposition of the TiO2 layer. The optical properties of layer with the antibody were optimized to achieve high sensitivity. Atomic force microscopy images of the sensor surface showed it to consist of a mesoporous structure with nano-scale unevenness. The binding of CRP to the sensor chips was monitored by RIfS, and CRP was repeatedly detected by this biosensor with a detection limit of ~60 ng mL−1. We presume that this two-layer method has a wide scope in that various kinds of biochips can be constructed for use in label-free optical biosensing.
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