Twenty-nine polymorphic microsatellite loci in Cyclura carinata, the Turks and Caicos Iguana, a critically endangered island endemic

Conservation Genetics Resources - Tập 3 - Trang 209-212 - 2010
Mark E. Welch1, G. June Long1,2, Jamen W. Berk1, Angela H. Getz1,3, Glenn P. Gerber4, Lisa E. Wallace1
1Department of Biological Sciences, Mississippi State University, Mississippi State, USA
2College of Veterinary Medicine, Auburn University, Auburn, USA
3Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, USA
4San Diego Zoo Institute for Conservation Research, Escondido, USA

Tóm tắt

Cyclura carinata, a critically endangered Caribbean rock iguana, now occupies less than 5% of its historic range. Remaining populations are genetically structured, but available tools are insufficient to identify taxonomic units. Ten polymorphic microsatellites isolated from C. carinata, and 19 loci developed for congeners are identified. All 29 loci were screened for variation in two populations with 20 or more individuals from each of two island populations, as well as 24 individuals that are representative of the species range.

Tài liệu tham khảo

An J, Sommer JA, Shore GD, Williamson JE, Brenneman RA, Louis EE (2004) Characterization of 20 microsatellite marker loci in the West Indian rock iguana (Cyclura nubila). Conserv Genet 5:121–125 Bryan JJ, Gerber GP, Welch ME, Stephen CL (2007) Re-evaluating the taxonomic status of the Booby Cay Iguana, Cyclura carinata bartschi. Copeia 2007:734–739 Don R, Cox P, Wainwright B, Baker K, Mattick J (1991) ‘Touchdown’ PCR to circumvent spurious priming during amplification. Nucleic Acids Res 19:4008 Gerber GP (2004) Cyclura carinata. In: IUCN red list of threatened species. IUCN—The World Conservation Union, Gland, Switzerland Gerber GP, Iverson JB (2000) Turks Island iguana, Cyclura carinata carinata. In: Alberts A (ed) West Indian iguana: status survey and conservation action plan. IUCN, Gland, Switzerland, pp 15–18 Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222 Iverson JB (1979) Behavior and ecology of the rock iguana Cyclura carinata Bull Fla State Mus Biol Sci 24:175–358 Lau J, Alberts AC, Chemnick LG, Gerber GP, Jones KC, Mitchell AA, Ryder OA (2009) Isolation and characterization of 23 polymorphic microsatellite loci for a West Indian iguana (Cyclura pinguis) from the British Virgin Islands. Mol Ecol Resour 9:1412–1414 Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295 Rosas KG, Pérez-Buitrago N, Acevedo JP, Martínez, Funk SM (2008) Development and characterization of 11 microsatellite loci for the Mona Island iguana (Cyclura cornuta stejnegeri). Mol Ecol Resour 8:825–827 Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386 Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234 Welch ME (1997) The population genetic structure of Cyclura carinata carinata (Sauria: Iguanidae). M.S. Thesis, University of Tennessee Welch ME, Gerber GP, Davis SK (2004) Genetic structure of the Turks and Caicos Rock Iguana and its implications for species conservation. In: Alberts AC, Carter RL, Hayes WH, Martins EP (eds) Iguanas, biology and conservation. University of California Press, Berkeley, pp 58–70