Tri-component diblock copolymers of poly(ethylene glycol)–poly(ε-caprolactone-co-lactide): synthesis, characterization and loading camptothecin
Tóm tắt
Biodegradable tri-component diblock copolymer was synthesized by bulk copolymerization of ε-caprolactone (CL) and D, L-lactide (LA) in the presence of methoxy poly(ethylene glycol) (MePEG), using stannous octoate as catalyst. Their chemical structure and physical properties were investigated by GPC, NMR, DSC, TGAand XRD. The increase of CL/LA ratio in the diblock copolymer leads to lower T
g, higher decomposition temperature and crystallinity. Nanoparticles formulated from MePEG–poly(CL-co-LA) (PCAE) possess spherical structure, which was characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The DLS results indicate that the particle size increased with the increase of CL/LA ratio and the hydrophobic fragment length in the copolymer. The drug encapsulation efficiency and the drug release behavior in vitro conditions of camptothecin were measured by high performance liquid chromatography (HPLC). The encapsulation efficiency can be achieved as high as 84.4% and the release behavior can be made well-controlled. MePEG–poly(CL-co-LA) nanoparticles might have a great potential as carriers for hydrophobic drugs.
Tài liệu tham khảo
Grislain L, Couvreur P, Lenaerts V, Roland M (1983) Int J Pharm 15:335
Stolnik S, Illum L, Davis SS (1995) Adv Drug Deliv Rev 16:195
Kataoka K, Harada A, Nagasaki Y (2001) Adv Drug Deliv Rev 53:113
Torchilin VP (2002) Adv Drug Deliv Rev 54:235
Gref R, Minamitake Y, Peracchia MT, Trubetskoy V, Torchilin VP, Langer R (1994) Science 263:1600
Gref R, Lueck M, Quellec P, Marchand M, Dellacherie E, Harnisch S, Blunk T, Muller RH (2000) Colloid Surf B Biointerfaces 18:301
Cai Q, Bei JZ, Wang SG (2002) Polymer 43:3585
Kumar N, Ravikumar MNV, Domb AJ (2001) Adv Drug Deliv Rev 53:23
Zhang Y, Zhang QZ, Zha LS et al (2004) Colloid Polym Sci 282:1323
Yamamoto YJ, Nagasaki YK, Kato YK, Sugiyama YC, Kataoka K (2001) J Control Rel 77:27
Yasugi K, Nagasaki Y, Kato M (1999) J Control Rel 62:89
Lucke A, Fustella E, Tessmar J et al (2002) J Control Rel 80(1–3):157
Dong YC, Feng SS (2004) Biomaterials 25:2843
Pitt CG (1990) Biodegradable polymers as drug delivery system. In: Chasin M, Langer R (eds) Marcel Dekker, New York, p 71
Shin ILG, Kim SY, Lee YM, Cho Chong S, Sung YK (1998) J Control Rel 51:1
Soo PL, Luo L, Maysinger D, Eisenberg A (2002) Langmuir 18:9996
Li SM, Garreau H, Bernard P, Jonathan MG, Alexandra T, Michel V (2002) Biomacromolecules 3:525
Tobyo M, Gref R, Sanchez A, Langer R, Alonso MJ (1998) Pharm Res 15:270
Liu JB, Xiao YH, Allen C (2004) J Pharm Sci 93(1):132
Jeong Y-I, Cheon J-B, Kim S-H et al (1998) J Control Rel 51:169
Cho HC, Chung DJ, Jeongho A (2004) Biomaterials 25:3733
Jackson JK, Zhang XC, Llewellen S, Hunter WL, Burt HM (2004) Int J Pharm 270:185
Wall ME, Wani MC, Cook CE et al (1966) J Am Chem Soc 88:3888
Potmesil M (1994) Cancer Res 54:1431
Giovanellea BC, Hinz HR, Kozieiski AJ et al (1991) Cancer Res 51:3052
Beijnen JH (1993) J Chromatogr Biomed Appl 617:111
Mohammadi-Rovshandeh J, Farnia SMF, Sarbolouki MN (2002) J Appl Polym Sci 83:2072
Huang MS, Li S, Coudane J, Vert M (2003) Macromol Chem Phys 204:1994
Lucke A, Tessmar J, Schnell E, Schmeer G, Gopferich A (2000) Biomaterials 21:2361
D‘Antone S, Bignotti F, Sartone L et al (2001) Polym Degrad Stab 74:119
Bignotti F, Penco M, Sartore L et al (2002) Macromol Symposia 180:257
Tuzar Z, Kratochvil P (1976) Adv Colloid Interf Sci 6:201
Hu Y, Jiang XQ, Ding Y, Zhang LY et al (2003) Biomaterials 24(13):2395
Izumikawa S, Yoshioka S, Aso Y, TakedaY (1991) J Control Rel 15:133
Allen C, Maysinger D, Eisenberg A (1999) Colloid Surf B Biointerfaces 16:3