Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells

Chapman, S., Oparka, K.J. & Roberts, A.G. New tools for in vivo fluorescence tagging. Curr. Opin. Plant Biol. 8, 565–573 (2005).

Hanson, M.R. & Kohler, R.H. GFP imaging: methodology and application to investigate cellular compartmentation in plants. J. Exp. Bot. 52, 529–539 (2001).

Lippincott-Schwartz, J. & Patterson, G.H. Development and use of fluorescent protein markers in living cells. Science 300, 87–91 (2003).

Miao, Y., Yan, P.K., Kim, H., Hwang, I. & Jiang, L. Localization of green fluorescent protein fusions with the seven Arabidopsis vacuolar sorting receptors to prevacuolar compartments in tobacco BY-2 cells. Plant Physiol. 142, 945–962 (2006).

Tse, Y.C. et al. Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells. Plant Cell 16, 672–693 (2004).

Pimpl, P., Hanton, S.L., Taylor, J.P., Pinto-daSilva, L.L. & Denecke, J. The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole. Plant Cell 15, 1242–1256 (2003).

Sheen, J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 127, 1466–1475 (2001).

Sparkes, I.A., Runions, J., Kearns, A. & Hawes, C. Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants. Nat. Protoc. 1, 2019–2025 (2006).

Walter, C., Smith, D.R., Connett, M.B., Grace, L. & White, D.W. A biolistic approach for the transfer and expression of a gusA. reporter gene in embryogenic cultures of Pinus radiata. Plant Cell Rep. 14, 69–74 (1994).

daSilva, L.L., Foresti, O. & Denecke, J. Targeting of the plant vacuolar sorting receptor BP80 is dependent on multiple sorting signals in the cytosolic tail. Plant Cell 18, 1477–1497 (2006).

Park, M., Lee, D., Lee, G.J. & Hwang, I. AtRMR1 functions as a cargo receptor for protein trafficking to the protein storage vacuole. J. Cell Biol. 170, 757–767 (2005).

Menges, M. & Murray, J.A. Synchronous Arabidopsis suspension cultures for analysis of cell-cycle gene activity. Plant J. 30, 203–212 (2002).

Nagata, T., Nemoto, Y. & Hasezawa, S. Tobacco BY-2 cell line as the 'HeLa' cell in the cell biology of higher plants. Int. Rev. Cytol. 132, 1–30 (1992).

Delhaize, E. et al. A role for the AtMTP11 gene of Arabidopsis in manganese transport and tolerance. Plant J. 51, 198–210 (2007).

Jiang, L. & Rogers, J.C. Integral membrane protein sorting to vacuoles in plant cells: evidence for two pathways. J. Cell Biol. 143, 1183–1199 (1998).

Kerppola, T.K. Visualization of molecular interactions by fluorescence complementation. Nat. Rev. Mol. Cell Biol. 7, 449–456 (2006).

Lippincott-Schwartz, J., Snapp, E. & Kenworthy, A. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2, 444–456 (2001).

Yoo, S.D., Cho, Y.H. & Sheen, J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat. Protoc. 2, 1565–1572 (2007).

Lam, S.K. et al. Rice SCAMP1 defines clathrin-coated, trans-Golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells. Plant Cell 19, 296–319 (2007).

Tse, Y.C., Lo, S.W., Hillmer, S., Dupree, P. & Jiang, L. Dynamic response of prevacuolar compartments to brefeldin A in plant cells. Plant Physiol. 142, 1442–1459 (2006).

Matsuoka, K. et al. A comprehensive gene expression analysis toward the understanding of growth and differentiation of tobacco BY-2 cells. Plant Cell Physiol. 45, 1280–1289 (2004).