Transforming growth factor‐β<sub>1</sub>‐induced expression of the mucosa‐related integrin α<sub>E</sub> on lymphocytes is not associated with mucosa‐specific homing

European Journal of Immunology - Tập 25 Số 6 - Trang 1487-1491 - 1995
F Austrup1, Sabine Rebstock1, Peter J. Kilshaw2, Alf Hamann1
1Abteilung für Immunologie, Medizinische Klinik, Universitätskrankenhaus Eppendorf, Hamburg, Germany
2Department of Cell Biology, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, GB

Tóm tắt

AbstractThe integrin αE (HML‐1, αIEL, αM290) is largely expressed on lymphocytes in epithelial sites, especially the gut mucosa. We investigated whether αE has any role in homing or delineates a phenotype with distinct migratory behavior. Lymph node T cells were stimulated for 5 days with anti‐CD3 in the presence or absence of transforming growth factor (TGF)‐β1 to generate αE+ or αE− cells, respectively. The two populations were then tested for their homing properties in mice. Both αE+ (TGF‐β‐treated) and αE− (control) cells of either CD4+ or CD8+ subset had a low capacity to enter the gut and showed the same homing behavior with respect to a variety of other organs. The same was true for αE+ and αE− cells that had been briefly stimulated with anti‐CD3 (24 h) and then allowed to return to a resting state before injection, though in this case both populations showed a greater capacity to recirculate through lymphoid tissue than was seen with fully activated cells. The results indicate that αEβ7 does not act as a homing receptor, and that the expression of the site‐specific marker αE does not correlate with a distinct homing behavior.

Từ khóa


Tài liệu tham khảo

Smith M. E., 1980, Monogr. Allergy, 16, 203

10.1007/978-3-642-71272-2_3

10.1016/0952-7915(93)90063-X

10.1007/978-3-642-78253-4_9

10.1016/0952-7915(94)90118-X

10.1002/eji.1830040610

10.1084/jem.148.5.1146

McDermott M. R., 1979, J. Immunol., 122, 1892, 10.4049/jimmunol.122.5.1892

Scicchitano R., 1988, Migration and homing of lymphoid cells, 1

Hamann A., 1992, Int. J. Cancer., 7, 19

Coffman R. L., 1986, J. Immunol., 136, 4538, 10.4049/jimmunol.136.12.4538

10.1084/jem.170.3.1039

10.1002/eji.1830211041

10.1002/eji.1830220140

10.1073/pnas.89.5.1924

10.1165/ajrcmb.10.3.7509610

10.3109/02713689409042401

10.1002/eji.1830240605

10.1002/eji.1830231246

10.1016/0092-8674(93)90305-A

Hamann A., 1994, J. Immunol., 152, 3282, 10.4049/jimmunol.152.7.3282

10.1038/372190a0

10.1002/eji.1830250333

10.1111/j.1600-065X.1983.tb01083.x

10.1111/j.1600-065X.1979.tb00289.x

10.1084/jem.150.3.580

Springer T. A., 1980, Monoclonal antibodies, 186

10.1073/pnas.84.5.1374

Andrew D. P., 1994, J. Immunol., 153, 3847, 10.4049/jimmunol.153.9.3847

10.1038/304030a0

10.1007/978-1-4684-5535-9_78

10.1083/jcb.126.1.247

Dejana E., 1994, J. Cell Biochem., 18, 295

10.1084/jem.148.6.1661

10.1016/0016-5085(93)90011-Z

Thông tin
Thông tin xuất bản

European Journal of Immunology

Tập 25 Số 6

1487-1491

Thông tin tác giả