Transformation of Medicago sativa L. Using a Ti Plasmid Derived Vector

Plant Breeding - Tập 106 Số 1 - Trang 39-46 - 1991
Mario Pezzotti1, Fulvio Pupilli1, F. Damiani1, S. Arcioni1
1Dr. M. Pezzotti, Dr. F. Pupilli, Dr. F. Damiani, Dr. S. Arcioni, Centre di Studio per il Miglioramento Genetico delle Piante Foreggere del C.N.R., Borgo XX Giugno 74, 1–06100 Perugia (Italia).

Tóm tắt

AbstractA cultivar of Medicago sativa was transformed with Agrobacterium tumefaciens strain At81 binary‐vector carrying the plasmid pKan3A, with the neomycin phosphotransferase gene under the control of the mannopine biosynthesis promoter. Stem segments were infected with the bacterium and kanamycin resistant calli were obtained; plant regeneration via somatic embryogenesis and polyembryogenesis was achieved only in media devoid of the antibiotic. Genetic transformation was confirmed by the presence of the structural gene through DNA‐DNA hybridization and the enzymatic assay showed its functional expression. Mesophyll protoplasts, leaf calli and S1 progeny of transformed plants grew in the presence of kanamycin (100 μgml‐1). Results are discussed in relation to the use of kanamycin‐resistant plants in somatic hybridization in the genus Medicago.

Từ khóa


Tài liệu tham khảo

10.1016/S0044-328X(82)80072-X

Arcioni S., 1989, Somatic embryogenesis and somaclonal variation in Medicago sativa, Genetics and Breeding, 43, 223

10.1073/pnas.71.9.3672

10.1007/BF00269243

10.1007/BF02860827

10.1007/BF02712670

10.1038/319791a0

10.1016/0014-4827(68)90403-5

10.1093/nar/11.1.159

10.1007/978-94-009-2788-9_56

10.1038/303179a0

10.1126/science.223.4635.496

Horsch J. Fry, 1988, Plant Molecular Biology Manual, A5/1

10.1007/BF00380613

10.1016/S0044-328X(80)80051-1

10.1007/BF00289819

Maniatis T., 1982, Molecular cloning: A laboratory manual

10.1111/j.1399-3054.1962.tb08052.x

10.1016/0378-1119(81)90146-3

Pezzotti M., 1984, Plant regeneration from mesophyll, root and cell suspension protoplasts of Medicago sativa cv. ‘Adriana’, Genet Agr., 38, 195

10.1002/j.1460-2075.1984.tb02297.x

Schenk R. V., 1972, Medium and techniques induction and growth of monocotyledonous and dicotyledonous plant cell cultures, Can. J. Bot., 50, 199, 10.1139/b72-026

10.2135/cropsci1986.0011183X002600060033x

10.1016/0304-4211(84)90140-8

10.1038/313358a0

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Plant Breeding

Tập 106 Số 1

39-46

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