Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

Plant Journal - Tập 22 Số 6 - Trang 531-541 - 2000
Anthony Trieu1,2,3, Stephen Burleigh1,4,3, Igor Kardailsky1,5,6,3, Ignacio E. Maldonado‐Mendoza3, Wayne K. Versaw3, Laura A. Blaylock3, Heungsop Shin3, Tzyy‐Jen Chiou3, Hiroaki Katagi3, Gary R. Dewbre3, Detlef Weigel6, Maria Harrison3
1First co-authors.
2Present address: Genomics Department, Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268-1054, USA.
3The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402, USA, and
4Present address: Centre for Plant-Microbe Symbioses, Risoe National Laboratory, Box 49, DK4000, Roskilde, Denmark.
5Present address: Plant Gene Expression Centre/USDA, 800 Buchanan Street, Albany, CA 94710, USA.
6The Salk Institute for Biological Studies, 10010 N. Torrey Pines Road, La Jolla, CA 92037, USA

Tóm tắt

SummaryTwo rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large‐scale insertional mutagenesis. T‐DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

Từ khóa


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Plant Journal

Tập 22 Số 6

531-541

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