Transfer of small chromosome fragments ofAgropyron elongatum to wheat chromosome via asymmetric somatic hybridization
Tóm tắt
The chromosome constitution of hybrids and chromatin patterns of Agropyron elongatum (Host)Neviski in F5 somatic hybrid lines -1–3 and I-1-9 between Triticum aestivum L. and A. elongatum were analyzed. Based on the statistic data of pollen mother cells, F5 I-1-9 and-1-3 had 20–21 bivalents with a frequency of 84.66% and 85.28%, of which, 89.83% and 89.57% were ring bivalents. The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior. RAPD analysis showed that the two hybrids contained biparental and integrated DNA. GISH (Genome in situ hybridization) revealed that in the form of small chromosome segments, A. elongatum chromatin was scattered on 4–6 wheat chromosomes near by the region of centromere and telomere in the two hybrid lines. SSR analysis indicated that A. elongatum DNA segments were distributed on the 2A, 5B, 6B and 2D wheat chromosomes in the hybrids, which was in accordance with the GISH results that small-segments intercalated poly-site.
Tài liệu tham khảo
Dong, Y. C., Genepools of common wheat, Journal of Triticeae Crops (in Chinese), 2000, 20 (3): 78–81.
Wei, Y. M., Zheng, Y. L., Zhou, R. H., Detection of the rye chromatin in multispikelet wheat germplasm 10-A background using fluorescence in situ hybridization (FISH) and RFLP markers, Acta Bot. Sinica (in Chinese), 1999, 41(7): 722–725.
Xiang, F. N., Xia, G. M., Chen, H. M., Effect of UV dosage on somatic hybridization between common wheat (Triticum aestivum L.) and Avena sativa L, Plant Sci., 2003, 164: 697–707
Ren, Z. L., Zhang, H. Q., Induce small-segment-transposition between wheat and rye chromosomes, Science in China, Ser. C, 1997, 40 (3): 323–331.
Hu, H., Zhang, X. Q., Zhang, W. J., Chromosome engineering of pollen wheat, Chin. Sci. Bull., 1999, 44(11): 964–970.
Li, Y. W., Li, H. J., Liang, H., Fluorescence in situ hybridization applied to the meiotic analysis and spontaneous chromosome translocation in the pollen mother cells of hybrids Triticum-Haynaldia, Acta Genet. Sin. (in Chinese), 2000, 27(4): 317–324.
Fehér, A., Preiszner, Z., Litkey, J. et al., Characterization of chromosome instability in interspecific somatic hybrids obtained by X-ray fusion between potatoes (Solanum tuberosum L.) in addition, S. brevidens Phil., Theor. Appl. Genet., 1992, 84: 880–890.
Zhou, A. F., Xia, G. M., Chen, H. M., Comparative study of symmetric and asymmetric somatic hybridization between common wheat and Haynaldia villosa, Science in China, Ser. C, 2001, 44 (3): 294–304
Xia, G. M., Wang, H., Chen, H. M., Plant regeneration for intergeneric asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Russian wildrye (Psathyrostichs juncea (Fisch) Neveski) and couch grass (Agropyron elongarum Host Neviski), Chin. Sci. Bull., 1996, 41(16): 1382–1386.
Xia, G. M., Xiang, F. N., Zhou, A. F. et al., Fertile transgenic plants production from intergeneric somatic hybridization between Triticum aestivum and Agropyron elongatum, Acta Bot. Sin. (in Chinese), 1999, 41(4): 328–331.
Chen, S.Y., Xia, G.M., Chen, H.M., Studies on NaCl-tolerant among somatic hybrid lines of wheat and Agropyron elangatum with their parents, Acta Bot. Boreali-Occidentalia Sin. (in Chinese), 2000, 20(3): 327–322.
Xiang, F. N., Feng, B. M., Xia, G. M. et al., Agronomie trait and protein component of F2 hybrid originated from intergeneric somatic hybridization between Triticum aestivum and Agropyron elongatum, Acta Bot. Sin., 2001, 43(3): 232–237.
Frederrick, M. A., Robert, E. K., Seidman, J. G. et al., Short Protocols in Molecular Biology, 3rd ed., Beijing: Science Press, 1998, 37–38.
Liu, L. Z., Chen, W. Q., Li, J. N., Exploration of reaction system of randomly amplified polymorphic DNA analysis in wheat, Biotech, (in Chinese), 2000, 10(3): 1–4.
Wang, J., Xiang, F. N., Xia, G. M. et al., Karyotype analysis of the F5 somatic hybrids between Triticum aestivum and Agropyron elongatum, J. Triticeae Crops (in Chinese), 2002, 23 (1): 12–16.
Zhou, H. P., Li, B., Li, Z. S., The study of breeding blue-grain genes translation of wheat, Acta Bot. Boreal. (in Chinese), 1995, 15 (2): 125–128.
Xia, G. M., Li, Z. Y., Wang, S. L. et al., Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa, Plant Sci., 1998, 137: 217–223
Forsberg, J., Dixelius, C., Lagercrantz, U. et al., UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana, Plant Sci., 1998, 131: 65–76
Zhou, A. F., Xia, G. M., Chen, H. M., Production of somatic hybrid plants between two types of wheat protoplasts and the protoplasts of Haynaldia villosa, Acta Bot. Sin., 2002, 44 (8): 1004–1008.
Xiang, F. N., Xia, G. M., Chen, H.M., Somatic hybridization between Triticum aestivum and Avena sativa, Science in China, Ser. C, 2003 46(3): 244–252
Zhou, K. C., Zhang, X. Q., Wang, X. P., Chromosomal location and molecular marker of resistance gene to puccinia strriiformis West in leymus mollis, Trin. Hara. (in Chinese), 2001, 28 (9): 864–869.
Zhang, X. Y., Li, D. Y., Repetitive DNA sequences in wheat and its relatives, Scientia Agri. Sin. (in Chinese), 2000, 33 (5): 1–7.
Zhang, X. Y., Dong, Y. Ch., Genome composition of Thinopyrum and rules of new species formation, J. Yunnan Uni. (Natural Science)(in Chinese), 1999, 21: 66–67.