Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors

Journal of Virological Methods - Tập 138 Số 1-2 - Trang 85-98 - 2006
Sharon E. Reed1, Elizabeth M. Staley2, John P. Mayginnes1, David J. Pintel1, Gregory E. Tullis3
1Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211, United States
2Cellular & Molecular Biology Program, University of Alabama at Birmingham, Birmingham, AL 35294, United States
3Department of Ophthalmology, Boston University, Boston, MA 02118, United States

Tóm tắt

Từ khóa


Tài liệu tham khảo

Bieber, 2001, Biotechniques, 30, 74, 10.2144/01301st03

Boeckle, 2004, Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer, J. Gene. Med., 6, 1102, 10.1002/jgm.598

Boussif, 1995, A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine, Proc. Natl. Acad. Sci. U.S.A., 92, 7297, 10.1073/pnas.92.16.7297

Breunig, 2005, Gene delivery with low molecular weight linear polyethylenimines, J. Gene Med., 7, 1287, 10.1002/jgm.775

Brunner, 2002, Overcoming the nuclear barrier: cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation, Mol. Ther., 5, 80, 10.1006/mthe.2001.0509

Collaco, 1999, A helper virus-free packaging system for recombinant adeno-associated virus vectors, Gene, 238, 397, 10.1016/S0378-1119(99)00347-9

Collaco, 2003, A method for helper virus-free production of adeno-associated virus vectors, Meth. Mol. Med., 76, 237

Derouazi, 2004, Serum-free large-scale transient transfection of CHO cells, Biotechnol. Bioeng., 87, 537, 10.1002/bit.20161

Drittanti, 2001, Optimised helper virus-free production of high-quality adeno-associated virus vectors, J. Gene Med., 3, 59, 10.1002/1521-2254(2000)9999:9999<::AID-JGM152>3.0.CO;2-U

Durocher, 2002, High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells, Nucl. Acids Res., 30, E9, 10.1093/nar/30.2.e9

Fischer, 1999, A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity, Pharm. Res., 16, 1273, 10.1023/A:1014861900478

Goula, 1998, Polyethylenimine-based intravenous delivery of transgenes to mouse lung, Gene Ther., 5, 1291, 10.1038/sj.gt.3300717

Goula, 1998, Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system, Gene Ther., 5, 712, 10.1038/sj.gt.3300635

Grimm, 2003, Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6, Mol. Ther., 7, 839, 10.1016/S1525-0016(03)00095-9

Grimm, 1998, Novel tools for production and purification of recombinant adenoassociated virus vectors, Hum. Gene Ther., 9, 2745, 10.1089/hum.1998.9.18-2745

Itaka, 2004, In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine, J. Gene Med., 6, 76, 10.1002/jgm.470

Matsushita, 1998, Adeno-associated virus vectors can be efficiently produced without helper virus, Gene Ther., 5, 938, 10.1038/sj.gt.3300680

Matsushita, 2004, The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production, J. Gen. Virol., 85, 2209, 10.1099/vir.0.79940-0

Mayginnes, J., in press. Quantitation of encapsidated recombinant adeno-associated virus DNA in crude cell lysates and tissue culture medium by quantitative, real-time PCR. J. Virol. Meth.

Merdan, 2002, Intracellular processing of poly(ethylene imine)/ribozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments, Pharm. Res., 19, 140, 10.1023/A:1014212630566

Pear, 1993, Production of high-titer helper-free retroviruses by transient transfection, Proc. Natl. Acad. Sci. U.S.A., 90, 8392, 10.1073/pnas.90.18.8392

Pollard, 1998, Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells, J. Biol. Chem., 273, 7507, 10.1074/jbc.273.13.7507

Salvetti, 1998, Factors influencing recombinant adeno-associated virus production, Hum. Gene Ther., 9, 695, 10.1089/hum.1998.9.5-695

Samulski, 1987, A recombinant plasmid from which an infectious adeno-associated virus genome can be excised in vitro and its use to study viral replication, J. Virol., 61, 3096, 10.1128/JVI.61.10.3096-3101.1987

Samulski, 1989, Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression, J. Virol., 63, 3822, 10.1128/JVI.63.9.3822-3828.1989

Sonawane, 2003, Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes, J. Biol. Chem., 278, 44826, 10.1074/jbc.M308643200

Tullis, 2000, Efficient replication of adeno-associated virus type 2 vectors: a cis-acting element outside of the terminal repeats and a minimal size, J. Virol., 74, 11511, 10.1128/JVI.74.24.11511-11521.2000

Wightman, 2001, Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo, J. Gene Med., 3, 362, 10.1002/jgm.187

Xiao, 1998, Production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus, J. Virol., 72, 2224, 10.1128/JVI.72.3.2224-2232.1998