Transcriptome analysis of the wheat–Puccinia striiformis f. sp. tritici interaction

Molecular Plant Pathology - Tập 9 Số 2 - Trang 157-169 - 2008
Tristan E. Coram1,2, Meinan Wang3, Xianming Chen1,2
1Department of Plant Pathology, Washington State University, Pullman, WA 99164‐6430, USA
2US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research Unit, Pullman, WA 99163, USA
3Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA

Tóm tắt

SUMMARYStripe rust [caused by Puccinia striiformis Westend. f. sp. tritici Eriks. (Pst)] is a destructive disease of wheat (Triticum aestivum L.) worldwide. Genetic resistance is the preferred method for control and the Yr5 gene, originally identified in Triticum spelta var. album, represents a major resistance (R) gene that confers all‐stage resistance to all currently known races of Pst in the United States. To identify transcripts associated with the Yr5‐mediated incompatible interaction and the yr5‐compatible interaction, the Wheat GeneChip was used to profile the changes occurring in wheat isolines that differed for the presence of the Yr5 gene after inoculation with Pst. This time‐course study (6, 12, 24 and 48 h post‐inoculation) identified 115 transcripts that were induced during the R‐gene‐mediated incompatible interaction, and 73 induced during the compatible interaction. Fifty‐four transcripts were induced in both interactions and were considered as basal defence transcripts, whilst 61 transcripts were specific to the incompatible interaction [hypersensitive response (HR)‐specific transcripts] and 19 were specific to the compatible interaction (biotrophic interaction‐specific transcripts). The temporal pattern of transcript accumulation showed a peak at 24 h after infection that may reflect haustorial penetration by Pst at ~16 h. An additional 12 constitutive transcript differences were attributed to the presence of Yr5 after eliminating those considered as incomplete isogenicity. Annotation of the induced transcripts revealed that the presence of Yr5 resulted in a rapid and amplified resistance response involving signalling pathways and defence‐related transcripts known to occur during R‐gene‐mediated responses, including protein kinase signalling and the production of reactive oxygen species leading to a hypersensitive response. Basal defence also involved substantial induction of many defence‐related transcripts but the lack of R‐gene signalling resulted in weaker response.

Từ khóa


Tài liệu tham khảo

10.1111/j.2517-6161.1995.tb02031.x

10.1111/j.1365-313X.2006.02837.x

10.1094/MPMI-19-0407

10.1093/bioinformatics/19.2.185

10.1146/annurev.arplant.56.032604.144224

10.1105/tpc.104.023382

10.1080/07060660509507230

Chen X., 1998, 9th International Wheat Genetics Symposium, 237

10.1111/j.1467-7652.2006.00208.x

10.1071/FP06250

10.1038/35081161

10.1016/j.pbi.2005.05.002

10.1094/MPMI.2004.17.6.654

10.1016/j.mib.2007.05.015

10.1073/pnas.2435133100

10.1094/MPMI-19-0711

10.1146/annurev.arplant.48.1.525

10.1111/j.1462-5822.2004.00361.x

10.1016/S0958-1669(03)00035-1

10.1023/A:1026592509060

10.1093/genetics/164.2.655

10.1094/PHYTO-97-9-1083

10.1093/biostatistics/4.2.249

10.1111/j.1439-0434.1995.tb00258.x

10.1139/g04-097

10.1146/annurev.arplant.48.1.251

10.1016/S0007-1536(62)80032-1

10.1016/0048-4059(77)90030-3

McIntosh R., 1998, 9th International Wheat Genetics Symposium, 1

10.1016/S0168-9452(01)00538-6

10.1111/j.1365-3059.2006.01385.x

10.1094/PD-70-173d

10.1007/s11103-004-0685-1

10.1007/BF00208314

10.1016/S0031-9422(99)00463-X

10.1094/MPMI.2000.13.2.159

Sculer M., 2003, Functional genomics of P450s, Annu. Rev. Plant Biol, 54, 629, 10.1146/annurev.arplant.54.031902.134840

10.1093/nar/gki123

10.1094/MPMI-20-8-0887

10.1111/j.1365-3059.1990.tb02509.x

Wellings C., 2004, 11th International Cereal Rusts and Powdery Mildew Conference, 39

10.1007/s00122-002-1109-8

10.1093/nar/gkg059

Thông tin
Thông tin xuất bản

Molecular Plant Pathology

Tập 9 Số 2

157-169

Thông tin tác giả