Transcriptional analysis of genes related to biofilm formation, stress-response, and virulence in Listeria monocytogenes strains grown at different temperatures

Luiza Pieta1, Flavia Brusch Garcia1, Gustavo Pelicioli Riboldi2, Luisa Abruzzi de Oliveira3, Ana Paula Guedes Frazzon4, Jeverson Frazzon1
1Graduate Program in Food Science and Technology, Food Science Institute (ICTA), Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil
2Molecular Microbiology Laboratory, Federal University of Healthy Science of Porto Alegre (UFCSPA), Porto Alegre, Brazil
3Department of Genetics, UFRGS, Porto Alegre, Brazil
4Department of Microbiology, UFRGS, Porto Alegre, Brazil

Tóm tắt

Từ khóa


Tài liệu tham khảo

Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250

Becker LA, Evans SN, Hutkins RW, Benson AK (2000) Role of sigma(B) in adaption of Listeria monocytogenes to growth at low temperature. J Bacteriol 182:7083–7087

Ben Slama R, Miladi H, Chaieb K, Bakhrouf A (2013) Survival of Listeria monocytogenes cells and the effect of extended frozen storage (−20 °C) on the expression of its virulence gene. Appl Biochem Biotechnol 170:1174–1183. doi: 10.1007/s12010-013-0253-8

Borucki MK, Peppin JD, White D, Loge F, Call DR (2003) Variation in biofilm formation among strains of Listeria mocytogenes. Appl Environ Microbiol 69:7336–7342

Burall LS, Laksanalamai P, Datta AR (2012) Listeria monocytogenes mutants with altered growth phenotypes at refrigeration temperature and high salt concentrations. Appl Environ Microbiol 78:1265–1272

Chan YC, Boor KJ, Wiedmann M (2007) SigmaB-dependent and SigmaB-independent mechanisms contribute to transcription of Listeria monocytogenes cold stress genes during cold shock and cold growth. Appl Environ Microbiol 73:6019–6029

Czuprynski CJ (2005) Listeria monocytogenes: silage, sandwiches and science. Anim Health Res Rev 6:211–217

Daines DA, Bothwell M, Furrer J et al (2005) Haemophilus influenzae luxS mutants form a biofilm and have increased virulence. Microb Pathog 39:87–89

Folsom JP, Siragusa GR, Frank JF (2006) Formation of biofilm at different nutrient levels by various genotypes of Listeria monocytogenes. J Food Prot 69:826–834

Garmyn D, Augagneur Y, Gal L, Vivant A-L, Piveteau P (2012) Listeria monocytogenes differential transcriptome analysis reveals temperature-dependent Agr regulation and suggests overlaps with other regulons. PLoS One 7:e43154

Gründling A, Burrack LS, Bouwer HG, Higgins DE (2004) Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence. Proc Natl Acad Sci USA 101:12318–12323

Gueriri I, Cyncynatus C, Dubrac S, Arana AT, Dussurget O, Msadek T (2008) The DegU orphan response regulator of Listeria monocytogenes autorepresses its own synthesis and is required for bacterial motility, virulence and biofilm formation. Microbiology 154:2251–2264

Habimana O, Meyrand M, Meylheuc T, Kulakauskas S, Briandet R (2009) Genetic features of resident biofilms determine attachment of Listeria monocytogenes. Appl Environ Microbiol 75:7814–7821

Harvey J, Keenan KP, Gilmour A (2007) Assessing biofilm formation by Listeria monocytogenes strains. Food Microbiol 24:380–392

Hofer E, dos Reis CMF, Hofer CB (2006) Sorovares de Listeria monocytogenes e espécies relacionadas, isoladas de material clínico humano. Rev Soc Bras Med Trop 39:32–37

Ivy RA, Wiedmann M, Boor KJ (2012) Listeria monocytogenes grown at 7 °C shows reduced acid survival and an altered transcriptional response to acid shock compared to L. monocytogenes grown at 37 °C. Appl Environ Microbiol 78:3824–3836

Kamp HD, Higgins DE (2011) A protein thermometer controls temperature-dependent transcription of flagellar motility genes in Listeria monocytogenes. PLoS Pathog. doi: 10.1371/journal.ppat.1002153 doi: 10.1371%2Fjournal.ppat.1002153#pmc_ext

Kathariou S (2002) Listeria monocytogenes virulence and pathogenicity, a food safety perspective. J Food Prot 65:1811–1829

Larsen MH, Koch AG, Ingmer H (2010a) Listeria monocytogenes efficiently invades Caco-2 cells after low-temperature storage in broth and on deli meat. Foodborne Pathog Dis 7:1013–1018

Larsen MH, Leisner JJ, Ingmer H (2010b) The chitinolytic activity of Listeria monocytogenes EGD is regulated by carbohydrates but also by the virulence regulator PrfA. Appl Environ Microbiol 76:6470–6476

Lemon KP, Freitag NE, Kolter R (2010) The virulence regulator PrfA promotes biofilm formation by Listeria monocytogenes. J Bacteriol 192:3969–3976

Liu D (2006) Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 55:645–659

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25:402–408

McNab R, Ford SK, El-Sabaeny A, Barbieri B, Cook GS, Lamont RJ (2003) LuxS-based signaling in Streptococcus gordonii: autoinducer 2 controls carbohydrate metabolism and biofilm formation with Porphyromonas gingivalis. J Bacteriol 185:274–284

Mello JF, Einsfeldt K, Frazzon APG, Costa M, Frazzon J (2008) Molecular analysis of the iap gene of Listeria monocytogenes isolated from cheeses in Rio Grande do Sul, Brazil. Braz J Microbiol 39:169–172

Moretro T, Langsrud S (2004) Listeria monocytogenes: biofilm formation and persistence in food-processing environments. Biofilms 1:107–121

Murray EJ, Kiley TB, Stanley-Wall NR (2009) A pivotal role for the response regulator DegU in controlling multicellular behavior. Microbiology 155:1–8

Nes F, Riboldi GP, Frazzon APG, D’Azevedo P, Frazzon J (2010) Antimicrobial resistance and investigation of the molecular epidemiology of Listeria monocytogenes in dairy products. Rev Soc Bras Med Trop 43:382–385

Neuhaus K, Satorhelyi P, Schauer K, Scherer S, Fuchs TM (2013) Acid shock of Listeria monocytogenes at low environmental temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans. BMC Genomics 14:285. doi: 10.1186/1471-2164-14-285

O’Neil HS, Marquis H (2006) Listeria monocytogenes flagella are used for motility, not as adhesins, to increase host cell invasion. Infect Immun 74:6675–6681

Ollinger J, Bowen B, Wiedmann M, Boor KJ, Bergholz TM (2009) Listeria monocytogenes SigmaB modulates PrfA-mediated virulence factor expression. Infect Immun 77:2113–2124

Pan Y, Breidt F Jr, Gorski L (2010) Synergistic effect of sodium chloride, glucose, and temperature on biofilm formation by Listeria monocytogenes serotype 1/2a and 4b strains. Appl Environ Microbiol 76:1433–1441

Renzoni A, Klarsfeld A, Dramsi S, Cossart P (1997) Evidence that PrfA, the pleiotropic activator of virulence genes in Listeria monocytogenes, can be present but inactive. Infect Immun 65:1515–1518

Riboldi GP, Mattos EP, Frazzon J (2013) Biogenesis of [Fe-S] Cluster in Firmicutes: an unexploited field of investigation. Antonie Van Leeuwenhoek 104:283–300

Rieu A, Briandet R, Habimana O, Garmyn D, Guzzo J, Piveteau P (2008) Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains. Appl Environ Microbiol 74:4491–4497

Salter MG, Conlon HE (2007) Extraction of plant RNA. Methods Mol Biol 362:309–314

Sela S, Frank S, Belausov E, Pinto R (2006) A mutation in the luxS gene influences Listeria monocytogenes biofilm formation. Appl Environ Microbiol 72:5653–5658

Shin JH, Brody MS, Price CW (2010) Physical and antibiotic stresses require activation of the RsbU phosphatase to induce the general stress response in Listeria monocytogenes. Microbiology 156:2660–2669

Suo Y, Huang Y, Liu Y, Shi C, Shi X (2012) The expression of Superoxide Dismutase (SOD) and a Putative ABC Transporter Permease is inversely correlated during biofilm formation in Listeria monocytogenes 4b G. PLoS One. doi: 10.1371/journal.pone.0048467

Trachoo N (2003) Biofilms and the food industry. Songklanakarin J Sci Technol 25:807–815

Van der Veen S, Abee T (2010) Importance of SigB for Listeria monocytogenes static and continuous-flow biofilm formation and disinfectant resistance. Appl Environ Microbiol 76:7854–7860

Williams T, Bauer S, Beier D, Kuhn M (2005) Construction and characterization of Listeria monocytogenes mutants with in-frame deletions in the response regulator genes identified in the genome sequence. Infect Immun 73:3152–3159