Transcript accumulation of carotenoid biosynthesis genes in the cyanobacterium Synechocystis sp. PCC 6803 during the dark-to-light transition is mediated by photosynthetic electron transport

Plant Biotechnology Reports - Tập 4 - Trang 149-155 - 2010
Jee-Youn Ryu1, Ji-Young Song1, Youngho Chung2, Young-Mok Park3,4, Wah Soon Chow5, Youn-Il Park1,4
1Department of Biological Sciences, College of Biological Science and Biotechnology, Chungnam National University, Daejeon, Korea
2Proteome Analysis Team, Korea Basic Science Institute, Daejeon, Korea
3Mass Spectrometer Research Center, Korea Basic Science Institute, Daejeon, Korea
4Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea
5Division of Plant Sciences, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University, Canberra, Australia

Tóm tắt

Expression of the genes for carotenoid biosynthesis (crt) is dependent on light, but little is known about the underlying mechanism of light sensing and signalling in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis). In the present study, we investigated the light-induced increase in the transcript levels of Synechocystis crt genes, including phytoene synthase (crtB), phytoene desaturase (crtP), ζ-carotene desaturase (crtQ), and β-carotene hydroxylase (crtR), during a dark-to-light transition period. During the dark-to-light shift, the increase in the crt transcript levels was not affected by mutations in cyanobacterial photoreceptors, such as phytochromes (cph1, cph2 and cph3) and a cryptochrome-type photoreceptor (ccry), or respiratory electron transport components NDH and Cyd/CtaI. However, treatment with photosynthetic electron transport inhibitors significantly diminished the accumulation of crt gene transcripts. Therefore, the light induction of the Synechocystis crt gene expression is most likely mediated by photosynthetic electron transport rather than by cyanobacterial photoreceptors during the dark-to-light transition.

Tài liệu tham khảo

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Plant Biotechnology Reports

Tập 4

149-155

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