Towards a Biohybrid Lung Assist Device: N-Acetylcysteine Reduces Oxygen Toxicity and Changes Endothelial Cells’ Morphology

Springer Science and Business Media LLC - Tập 10 - Trang 153-161 - 2016
Tobias Plein1,2, Anja Lena Thiebes1,2, Nicole Finocchiaro1,2, Felix Hesselmann3, Thomas Schmitz-Rode1,3,2, Stefan Jockenhoevel1,2, Christian G. Cornelissen1,4,2
1Department of Biohybrid & Medical Textiles (BioTex) at AME-Helmholtz Institute for Biomedical Engineering, ITA-Institut für Textiltechnik, RWTH Aachen University, Aachen, Germany
2Aachen-Maastricht-Institute for Biobased Materials (AMIBM), Brightlands Chemelot Campus, Maastricht University, Geleen, The Netherlands
3Department of Cardiovascular Engineering, Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, Aachen, Germany
4Department for Internal Medicine – Section for Pneumology, Medical Faculty, RWTH Aachen University, Aachen, Germany

Tóm tắt

The development of an endothelialized membrane oxygenator requires solution strategies combining the knowledge of oxygenators with endothelial cells’ biology. Since it is well known that exposing cells towards pure oxygen causes oxidative stress, this aspect has to be taken into account in the development of a biohybrid oxygenator system. N-Acetylcysteine (NAC) is known for its antioxidant properties in cells. We tested its applicability for the development of an endothelialized oxygenator model. Cultivating human umbilical vein derived endothelial cells (HUVEC) up to 6 days with increasing concentrations of NAC from 1 to 30 mM revealed NAC toxicity at concentrations from 20 mM. Cell density clearly decreased after radical oxygen species exposure in non-NAC pretreated cells compared to 20 mM NAC precultured HUVEC after 3 and 6 days. Also the survival rate after ROS treatment could be restored by incubation with NAC from 15 to 25 mM for all time points. NAC treated cells changed their morphology from typical endothelial cells’ cobblestone pattern to a fusiform, elongated configuration. Transformed cells were still positive for typical endothelial cell markers. Our present results show the potential of NAC for the protection of an endothelial cell layer in an endothelialized membrane oxygenator due to its antioxidative properties. Moreover, NAC induces a morphological change in HUVEC similar to dynamic cultivation procedures.

Tài liệu tham khảo

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Springer Science and Business Media LLC

Tập 10

153-161

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