Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa

Walter de Gruyter GmbH - Tập 7 Số 6 - Trang 1116-1122 - 2012
Jelena S. Matejić1, Ana M. Džamić2, Tatjana Mihajilov‐Krstev3, Vladimir Ranđelović3, Zoran Krivošej4, Petar D. Marin2
1Faculty of Medicine, University of Niš, 18000, Niš, Serbia
2Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, 11000, Belgrade, Serbia
3Faculty of Science and Mathematics, University of Niš, 18000, Niš, Serbia
4Department of Biology, Faculty of Natural Sciences, University of Pristina, 38220, Kosovska Mitrovica, Serbia

Tóm tắt

Abstract The present study describes the total phenolic content, concentrations of flavonoids and in vitro antioxidant and antimicrobial activity of methanol extracts from Seseli pallasii Besser, S. libanotis (L.) Koch ssp. libanotis and S. libanotis (L.) Koch ssp. intermedium (Rupr.) P. W. Ball, growing wild in Serbia. The total phenolic content in the extracts was determined using Folin-Ciocalteu reagent and their amounts ranged between 84.04 to 87.52 mg GA (gallic acid)/g. The concentrations of flavonoids in the extracts varied from 4.75 to 19.37 mg Qu (quercetin)/g. Antioxidant activity was analyzed using DPPH reagent. Antioxidant activity ranged from 0.46 to 4.63 IC50 (mg/ml) and from 1.98 to 2.19 mg VitC (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively, using BHA and VitC as controls. The antimicrobial activity of the extracts was investigated using a micro-well dilution assay for the most common human gastrointestinal pathogenic bacterial strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis ATCC 13076, Bacillus cereus ATCC 10876, Listeria monocytogenes ATCC15313, Staphylococcus aureus ATCC 25923 and Candida albicans ATCC 10231. This finding suggests that Seseli species may be considered as a natural source of antioxidants and antimicrobial agents.

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Walter de Gruyter GmbH

Tập 7 Số 6

1116-1122

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