Time‐dependent effects of anesthetic agents on <sup>31</sup>P NMR high‐energy phosphates in KHT and RIF‐1 fibrosarcomas

NMR in Biomedicine - Tập 4 Số 3 - Trang 137-141 - 1991
Bruce M. Fenton1, Richard F. Raubertas2, Robert M. Sutherland3
1Experimental Therapeutics Division, University of Rochester Cancer Center, Rochester. NY 14642, USA
2Division of Biostatistics, University of Rochester Medical Center, Rochester, NY 14642, USA
3Cancer Biology Department, SRI International, Menlo Park, CA 94025, USA

Tóm tắt

AbstractPrevious studies have reported significant radiobiological and hemodynamic effects associated with sodium pentobarbital (PB) anesthetization. The present work contrasts the effects of PB with azaperone‐ketamine (AZ) in RIF‐1 and KHT tumors while animal body core temperature is maintained at 37 °C. The primary aims were to evaluate both agents in terms of: (i) duration of anesthetic; (ii) effect on absolute levels of 31P NMR phosphocreatine (PCr) + β‐nucleoside triphosphate (β‐NTP)/inorganic phosphate (Pi) ratios; and (iii) effect on temporal variability of PCr+β‐NTP/Pi ratios. In terms of overall duration, AZ was the clear preference. Although the maintenance of 37 °C core temperature significantly reduced overall durations for both anesthetics, AZ animals invariably remained immobile for a minimum of 80 min. For PB, durations were highly unpredictable. With AZ, mean PCr + β‐NTP/Pi ratios were constant over the entire 80 min period for both lines. With PB, PCr + β‐NTP/Pi ratios were lower in relation to AZ for KHT at select timepoints, but highly variable among RIF‐1 tumours. Since ratios under PB varied substantially with time for RIF‐1 lines, measurements taken with PB are clearly not representative of the control state. Furthermore, in light of the consistent and reproducible results obtained with AZ, this anesthetic is considered a marked improvement over PB for animal studies of this nature.

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NMR in Biomedicine

Tập 4 Số 3

137-141

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