Three serendipitous pathways in E. coli can bypass a block in pyridoxal‐5′‐phosphate synthesis
Tóm tắt
Bacterial genomes encode hundreds to thousands of enzymes, most of which are specialized for particular functions. However, most enzymes have inefficient promiscuous activities, as well, that generally serve no purpose. Promiscuous reactions can be patched together to form multistep metabolic pathways. Mutations that increase expression or activity of enzymes in such serendipitous pathways can elevate flux through the pathway to a physiologically significant level. In this study, we describe the discovery of three serendipitous pathways that allow synthesis of pyridoxal‐5′‐phosphate (PLP) in a strain of
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Tài liệu tham khảo
Dempsey WB, 1987, Synthesis of pyridoxal phosphate, Escherichia coli and Salmonella typhimurium; Cellular and Molecular Biology, 539