Three-dimensional reconstructions from non-deparaffinized tissue sections

Anatomy and Embryology - Tập 210 - Trang 163-173 - 2005
Marie Jirkovská1, Iva Náprstková2, Jiří Janáček3, Tomáš Kučera1, Jaroslav Macášek1, Petr Karen3, Lucie Kubínová3
1Institute of Histology and Embryology, 1st Faculty of Medicine, Charles University, Prague 2, Czech Republic
2Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic
3Institute of Physiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic

Tóm tắt

Three-dimensional (3-D) reconstruction from microscopic images represents a useful tool for the study of biological structures in embryology and developmental biology. However, it is usually necessary to cope with many difficulties connected with the preparation of specimens. In order to minimize mutual displacement of structures in successive sections, the applicability of non-deparaffinized tissue sections for 3-D reconstruction was tested. Chicken embryos were fixed and stained in toto with eosin and then embedded in paraffin. About 30-μm-thick non-deparaffinized serial sections were used for obtaining initial data for 3-D reconstruction of larger stacks of embryonic bodies using either fluorescence or confocal microscope. The same sections served for both collecting optical serial sections of mesonephros as source images for its 3-D reconstruction, and immunohistochemical detection of fibronectin, laminin and vimentin. It was found that sections with retained paraffin preserve the mutual spatial relationships of tissue components as well as provide an excellent differentiation of structure. It makes the process of 3-D reconstruction easier. The localization of the products of immunohistochemical reactions demonstrated the co-localization of fibronectin and laminin in basal laminas and the presence of vimentin in glomeruli and mesenchymal tissue. The use of non-deparaffinized sections represents a less time consuming and more effective alternative to thin histological sections for the purpose of 3-D reconstruction, and enables further application of material.

Tài liệu tham khảo

Åslund N, Carlsson K, Liljeborg A, Majlof L (1983) PHOIBOS, a microscope scanner designed for micro-fluorometric applications, using laser induced fluorescence. In: Proceedings of the third Scandinavian conference on image analysis, Studentliteratur, Lund, p 338 Apgar JM, Juarranz A, Espada J, Villanueva A, Caňete M, Stockert JC (1998) Fluorescence microscopy of rat embryo sections stained with haematoxylin-eosin and Masson’s trichrome method. J Microsc 191:20–27 Arnold WH, Lang T (2001) Development of the membranous labyrinth of human embryos and fetuses using computer aided 3D- reconstruction. Ann Anat 183:61–66 Arnold WH, Lang M, Sperber GH (2001) 3D- reconstruction of craniofacial structures of a human anencephalic fetus. Case report. Ann Anat 183:67–71 Baba N (1992) Computer aided three-dimensional reconstruction from serial section images. In: Häder DP (ed) Image analysis in biology. CRC Press, Boca Raton, pp 251–270 Beck JC, Murray JA, Willows AO, Cooper MS (2000) Computer-assisted visualizations of neural networks: expanding the field of view using seamless confocal montaging. J Neurosc Methods 98:155–163 Brakenhoff GJ, van der Voort HTM, van Spronsen EA, Linnemans WAM, Nanninga N (1985) Three dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy. Nature 317:748–749 Bron C, Gremillet P (1992) 3-D reconstructions by image-processing of serial sections in electron microscopy. In: Kriete A (ed) Visualization in biomedical microscopies. 3-D Imaging and computer applications. VCH, Weinheim, pp 75–105 Carlsson K, Danielsson P, Lenz R, Liljeborg A, Majlof L, Å slund N (1985) Three-dimensional microscopy using a confocal laser scanning microscope. Opt Lett 10:53–55 Carretero A, Ditrich H, Pérez-Aparicio FJ, Splechtna H, Ruberte J (1995) Development and degeneration of the arterial system in the mesonephros and metanephros of chicken embryo. Anat Rec 243:120–128 Carretero A, Ditrich H, Navarro M, Ruberte J (1997) Afferent portal venous system in the mesonephros and metanephros of chick embryos: development and degeneration. Anat Rec 247:63–70 Clarke GE, Hamilton PW, Montgomery WA (1993) Aligning histological serial sections for three-dimensional reconstruction using an excimer laser beam. Pathol Res Pract 189:563–566 Deverell MH, Whimster WF (1989) A method of image registration for three-dimensional reconstruction of microscopic structures using an IBAS 2000 image analysis system. Pathol Res Pract 185:602–605 Ditrich H, Lametschwandtner A (1992) Glomerular development and growth of the renal blood vascular system in Xenopus laevis (Amphibia: Anura: Pipidae) during metamorphic climax. J Morphol 213:335–340 Ditrich H, Splechtna H (1990) Kidney structure investigations using scanning electron microscopy of corrosion casts—a state of art review. Scan Microsc 4:943–956 Fraser AM, Swinney HL (1986) Independent coordinates for strange attractor from mutual information. Phys Rev A 33:1134–1140 Friebová Z (1975) Formation of the chick mesonephros. 1. General outline of development. Fol Morphol (Prague) 23:19–28 Friebová-Zemanová (1981) Formation of the chick mesonephros. 4. Course and architecture of the developing nephrons. Anat Embryol 161:341–354 Friebová-Zemanová Y, Goncharevskaya OA (1982) Foramtion of the chick mesonephros. 5. Spatial distribution of the nephron populations. Anat Embryol 165:125–139 Fujimura A, Nozaka Y (2002) Analysis of the three-dimensional lymphatic architecture of the periodontal tissue using a new 3D reconstruction method. Microsc Res Tech 56:60–65 Glaser EM, Van Der Loos H (1965) A semi-automatic computer microscope for the analysis of neuronal morphology. IEEE Trans Biomed Eng 2:22–31 Guo M, Ditrich H, Splechtna H (1996) Renal vascular and uriniferous tubules in the common iguana. J Morphol 229:97–104 Hell S, Reiner G, Cremer C, Stelzer EHK (1993) Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J Microsc 169:391–405 Hibbard LS, Grothe RA, Arnicar-Sulze TL, Dovey-Hartman BJ, Page RB (1993) Computed three-dimensional reconstruction of median-eminence capillary modules: image alignment and correlation. J Microsc 171:39–56 Hiruma T, Nakamura H (2003) Origin and development of the pronephros in the chick embryo. J Anat 203:539–552 Huijsmans DP, Lamers WH, Los JA, Strackee J (1986) Toward computerized morphometric facilities: a review of 58 software packages for computer-aided three-dimensional reconstruction, quantification, and picture generation from parallel serial sections. Anat Rec 216:449–470 Janáček J, Kubínová L (1998) 3D reconstruction of surfaces captured by confocal microscopy. Acta Stereol 17:259–264 Jirkovská M, Kubínová L, Krekule I, Karen P, Palovský R (1994) To the applicability of common fluorescent dyes in confocal microscopy. Funct Develop Morphol 4:171–172 Jirkovská M, Kubínová L, Krekule I, Hach P (1998) Spatial arrangement of fetal placental capillaries in terminal villi: a study using confocal microscopy. Anat Embryol 197:263–272 Jirkovská M, Kubínová L, Janáček J, Moravcová M, Krejčí V, Karen P (2002) Topological properties and spatial organization of villous capillaries in normal and diabetic placentas. J Vasc Res 39(3):268–278 Jirsová Z, Zemanová Z, Jirkovská M (2003) Development of glomerular and peritubular vascular bed of the chick mesonephros. Plzeň Lék Sborn 78:111–113 Karen P, Jirkovská M, Tomori Z, Demjénová E, Janáček J, Kubínová L (2003) Three dimensional computer reconstruction of large tissue volumes based on composing series of high-resolution confocal images by GlueMRC and LinkMRC software. Microsc Res Tech 62:415–422 Kaufman MH, Kaufman DB, Brune RM, Stark M, Armstrong JF, Clarke AR (1997) Analysis of fused maxillary incisor dentition in p53-deficient exencephalic mice. J Anat 191:57–64 Kay PA, Robb RA, Bostwick DG (1998) Prostate cancer microvessels: a novel method for three-dimensional reconstruction and analysis. Prostate 37:270–277 Knabe W, Washausen S, Brunnett G, Kuhn HJ (2002) Use of ’reference series’ to realign histological serial sections for three-dimensional reconstructions of the positions of cellular events in the developing brain. J Neurosc Meth 121:169–180 Kriete A (eds) (1992) Visualization in biomedical microscopies. 3-D Imaging and computer applications. Weinheim VCH, New York Kriete A, Magdowski G (1990) Computerized three-dimensional reconstructions of serial sections in electron microscopy. Ultramicroscopy 32:48–54 Kriete A, Schwebel T (1996) 3-D TOP-A software package for the topological analysis of image sequences. J Struct Biol 116:150–154 Kropf N, Sobel I, Levinthal C (1985) Serial section reconstruction using CARTOS. In: Mize RR (ed) The microcomputer in cell and neurobiology research. Elsevier, New York, pp 265–292 Levinthal C, Ware R (1972) Three dimensional reconstruction from serial sections. Nature 236:207–210 Lillie FR (1908) In: Hamilton HL (1952) Lillie’s Development of the Chick. Holt and Co, New York, p 470 Lorensen WE, Cline HE (1987) Marching cubes: a high resolution 3D surface construction algorithm. Comput Graph 21:163–169 Majlof L, Forsgren P-O (1993) Confocal microscopy: important consideration for accurate imaging. In: Matsumoto B (eds) Methods in cell biology, vol 38. Cell biological applications of confocal microscopy. Academic, New York Manconi F, Markham R, Cox G, Kable E, Fraser IS (2001) Computer-generated, three-dimensional reconstruction of histological parallel serial sections displaying microvascular and glandular structures in human endometrium. Micron 32:449–453 Murphy KM, Zalik SE (1999) Endogenous galectins and effect of galectin hapten inhibitors on the differentiation of the chick mesonephros. Dev Dyn 215:248–263 Nelsen, OE (1953) Comparative embryology of the vertebrates. Blakiston Comp, New York, pp 777–782 Oldmixon EH, Carlsson K (1993) Methods for large data volumes from confocal scanning laser microscopy of lung. J Microsc 170:221–228 Papadimitriou C, Yapijakis C, Davaki P (2004) Use of truncated pyramid representation methodology in three-dimensional reconstruction: an example. J Microsc 214:70–75 Pentecost JO, Icardo J, Thornburg KL (1999) 3D computer modeling of human cardiogenesis. Comput Med Imaging Graph 23:45–49 Pentecost JO, Sahn DJ, Thornburg BL, Gharib M, Baptista A, Thornburg KL (2001) Graphical and stereolitographic models of the developing human heart lumen. Comput Med Imaging Graph 25:459–463 Scarborough J, Aiton JF, McLachlan JC, Smart SD, Whiten SC (1997) The study of early human embryos using interactive 3-dimensional computer reconstructions. J Anat 191(Pt1):117–122 Sheppard CJR, Török, P (1997) Effects of specimen refractive index on confocal imaging. J Microsc 185:366–374 Shumway W, Adamstone FB (1954) Introduction to vertebrate embryology. Wiley, New York, p 208 Čapek M, Krekule I (1999) Alignment of adjacent picture frames captured by a CLSM. IEEE Trans Inform Technol Biomed 3:119–124 Vazquez M-D, Bouchet P, Mallet J-L, Foliquet B, Gérard H, LeHeup B (1998) 3D reconstruction of the mouse mesonephros. Anat Histol Embryol 27:283–287 Young AA, Legrice IJ, Young MA, Smaill BH (1998) Extended confocal microscopy of myocardial laminae and collagen network. J Microsc 192:139–150