Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies in Haloxylon persicum (Bunge ex Boiss & Buhse): an endangered desert tree species
Tóm tắt
Haloxylon persicum (Bunge ex Boiss & Buhse), is one of the hardy woody desert shrubs, which is now endangered and/or nearing extinction. Urban landscape development and overgrazing are the major threats for the erosion of this important plant species. For conserving the species, it is critical to develop an efficient in vitro regeneration protocol for rapid multiplication of large number of regenerants. Leaf explants, cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) (0.5, 1, 2 µM), showed significant difference in bud sprouting and adventitious shoot induction. The highest shoot bud formation was recorded on MS medium supplemented with 0.5 µM TDZ. Shoot tip necrosis (STN), observed after first subculture of shoot buds in same medium, increased in severity with subculture time. Application of calcium (4 mM) and boron (0.1 mM) in combination with kinetin (10 µM) in the subculture medium significantly reduced the intensity of STN. On an average eight shoots/explant were produced by alleviating this problem. ISSR marker analysis revealed monomorphic banding pattern between progenies and parents, indicating the true to type nature of the clones and its parents.
Tài liệu tham khảo
Advina LJ, Ranjetta P, Razip S, Sreeramanan S (2012) Histological analyses of PLBs of Dendrobium sonia-28 in the recognition of cell competence for regeneration and Agrobacterium infection. Plant Omics 5(6):514–517
Al-Khalifah NS (2004) The role of biotechnology in developing plant resources in deserts environment. Paper presented at the international conference on water resources and arid environment. King Saud University, Riyadh, Saudi Arabia
Bairu MW, Jain N, Stirk WA, Dolezal K, Van Staden J (2009) Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium. S Afr J Bot 75(1):122–127. https://doi.org/10.1016/j.sajb.2008.08.006
Birnbaum PEH (2001) In vitro propagation of Haloxylon aphyllum (minkw.) iljin. ISHS Acta Horticulturae 560. In: IV International symposium on in vitro culture and horticultural breeding, pp 461–464
Birnbaum PEH, Orlovsky NS, Nikolaev MV, Dourokov M (2000) Clonal propagation of the desert shrub, Haloxylon, for pasture improvement in Central Asia. http://pdf.usaid.gov/pdf_docs/PNACW403.pdf. Accessed 20 Mar 2018
Butnik AA, Nigmanova RN, Paizeiva SA, Saidov DK (1991) Ecological anatomy of desert plants of Middle Asia. 5.1. Trees, shrubs, semishrubs. Taskent: FAN (In Russian)
Casati P, Andrew CS, Edward GE (1999) Characterization of NADP-malic enzyme from two species of Chenopodiaceae: Haloxylon persicum (C4) and Chenopodium album (C3). Phytochemistry 52(6):985–992. https://doi.org/10.1016/S0031-9422(99)00355-6
Ferguson BJ, Beveridge CA (2009) Roles for auxin, cytokinin, and strigolactone in regulating shoot branching. Plant Physiol 149(4):1929–1944. https://doi.org/10.1104/pp.109.135475
Gairi A, Rashid A (2004) Direct differentiation of somatic embryos on different regions of intact seedlings of Azadirachta in response to thidiazuron. J Plant Physiol 161(9):1073–1077. https://doi.org/10.1016/j.jplph.2004.05.001
Gnasekara P, Mahmood M, Subramaniam S (2016) Ultrastructure study of Vanda Kasem’s Delight orchid’s protocorm-like body. Hortic Bras 34(3):333–339. https://doi.org/10.1590/S0102-05362016003005
Grigoriadou K, Leventakis N, Vasilakakis M (2000) Effects of various culture conditions on proliferation and shoot tip necrosis in the pear cultivars ‘William’s’ and ‘Highland’ grown in vitro. Acta Hortic 520(520):103–108. https://doi.org/10.17660/ActaHortic.2000.520.10
Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10(45):8984–9000. https://doi.org/10.5897/AJB11.636
Hepler PK (2005) Calcium: a central regulator of plant growth and development. Plant Cell 17(8):2142–2155. https://doi.org/10.1105/tpc.105.032508
Hirschi KD (2004) The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiol 136(1):2438–2442. https://doi.org/10.1104/pp.104.046490
Huetteman CA, Preece JE (1993) Thidiazuron—a potent cytokinin for woody plant-tissue culture. Plant Cell Tissue Organ Cult 33(2):105–119. https://doi.org/10.1007/BF01983223
Iljin MM (1936) Chenopodiaceae. In: Komorov VL, Shishkin BK (eds) Flora of the USSR, vol 6. (English translation by Landau N 1970). Israel Program for Scientific Translation, Jerusalem, pp 4–272
Jain N, Bairu MW, Stirk WA, Dolezal K, Van Staden J (2009) The effect of medium, carbon source and explant on regeneration and control of shoot-tip necrosis in Harpagophytum procumbens. S Afr J Bot 75(1):117–121. https://doi.org/10.1016/j.sajb.2008.08.005
Jones MPA, Cao J, O’Brien R, Murch SJ, Saxena PK (2007) The mode of action of thidiazuron: auxins, indoleamines, and ion channels in the regeneration of Echinacea purpurea L. Plant Cell Rep 26(9):1481–1490. https://doi.org/10.1007/s00299-007-0357-0
Kärkönen A (2000) Anatomical study of zygotic and somatic embryos of Tilia cordata. Plant Cell Tiss Organ Cult 61(3):205–214. https://doi.org/10.1023/A:1006455603528
Kataeva NV, Alexandrova IG, Butenko RG, Dragavtceva EV (1991) Effect of applied and internal hormones on vitrification and apical necrosis of different plants cultured in vitro. Plant Cell Tiss Organ Cult 27(2):149–154. https://doi.org/10.1007/BF00041283
Kurup SS, Aly MAM, Lekshmi G, Tawfik NH (2014) Rapid in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Kheneizi using tender leaf explant. Emir J Food Agric 26(6):539–544. https://doi.org/10.9755/ejfa.v26i6.1805
Lakshmi SG, Raghava SBV (1993) Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci 88(1):107–112. https://doi.org/10.1016/0168-9452(93)90115-G
Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol Plant 29(2):92–96. https://doi.org/10.1007/BF02632259
Mandaville JP (1986) Plant life in the Rub’al-Khali (Empty Quarter) south central Arabia. Proc R Soc Edinb 89B:147–157
McCown BH, Sellmer JC (1987) General media and vessels suitable for woody plant culture. In: Bonga JM, Durzan L (eds) Cell and tissue culture in forestry. General principles and biotechnology, vol 1. Martinus Nijhoff, Dordrecht, pp 4–16
Mohamed MAH, Assaeed AM, Yousuf HN (2013) Micropropagation of the endangered desert shrub Haloxylon persicum. Aust J Crop Sci 7(2):255–260
Murch SJ, Krishna Raj S, Saxena PK (2000) Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John’s wort (Hypericum perforatum L. cv. Anthos) plants. Plant Cell Rep 19(7):698–704. https://doi.org/10.1007/s002990000206
Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275. https://doi.org/10.1007/BF02822732
Neto VBP, Otoni WC (2003) Carbon sources and their osmotic potential in plant tissue culture: does it matter? Sci Hortic 97(3–4):193–202. https://doi.org/10.1016/S0304-4238(02)00231-5
Parveen S, Shahzad A (2010) TDZ-induced high frequency shoot regeneration in Cassia sophera Linn. via cotyledonary node explants. Physiol Mol Biol Plants 16(2):201–206. https://doi.org/10.1007/s12298-010-0022-x
Piagnani C, Zocchi G, Mignani I (1996) Influence of Ca2 + and 6-benzyladenine on chestnut (Castanea sative Mill.) in vitro shoot-tip necrosis. Plant Sci 118(1):89–95. https://doi.org/10.1016/0168-9452(96)04423-8
Pradeep RM, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128(1):9–17. https://doi.org/10.1023/A:1020691618797
Rathore JS, Rathore V, Shekhawat NS, Singh RP, Liler G, Phulwaria M, Dagla HR (2005) Micropropagation of woody plants. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Springer, Berlin
Ruan X, Wang Q, Chen Y, Huang J (2006) Physio-ecological response of Haloxylon persicum photosynthetic shoots to drought stress. Front For China 1(2):176–181. https://doi.org/10.1007/s11461-006-0021-9
Seling S, Wissemeier AH, Cambier P, Van Cutsem P (2000) Calcium deficiency in potato (Solanum tuberosum ssp. tuberosum) leaves and its effects on the pectic composition of the apoplastic fluid. Physiol Plant 109(1):44–50. https://doi.org/10.1034/j.1399-3054.2000.100107.x
Soumen S, Sinchan A, Tulsi D, Parthadeb G (2016) RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1. Meta Gene 7:7–15. https://doi.org/10.1016/j.mgene.2015.10.004
Tiwari JK, Poonam C, Shruti G, Gopal Jai, Singh BP, Vinay B (2013) Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers. Physiol Mol Biol Plants 19(4):587–595. https://doi.org/10.1007/s12298-013-0190-6
Venkatachalam L, Sreedhar RV, Bhagyalakshmi N (2007) Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers. Electron J Biotechnol 10(1):106–113. https://doi.org/10.2225/vol10-issue5-fulltext-12
Victor JMR, Murch SJ, KrishnaRaj S, Saxena PK (1999) Somatic embryogenesis and organogenesis in peanut: the role of thidiazuron and N-6-benzylaminopurine in the induction of plant morphogenesis. Plant Growth Regul 28(1):9–15. https://doi.org/10.1023/A:1006274615736
Vieitez AM, Sanchez C, San-Jose C (1989) Prevention of shoot tip necrosis in shoot cultures of chestnut and oak. Sci Hortic 41(1–2):101–109. https://doi.org/10.1016/0304-4238(89)90059-9
Wang H, Van Staden J (2001) Establishment of in vitro cultures of tree peonies. S Afr J Bot 67(2):358–361. https://doi.org/10.1016/S0254-6299(15)31141-8
Wesam AK, Eman B, Jamil L, Dana S, Emad Hu (2013) Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using inter simple sequence repeat (ISSR). Physiol Mol Biol Plants 19(1):157–164. https://doi.org/10.1007/s12298-012-0149-z
Yuan XF, Dai ZH, Wang XD, Zhao B (2009) Assessment of genetic stability in tissue-cultured products and seedlings of Saussurea involucrata by RAPD and ISSR markers. Biotechnol Lett 31(8):1279–1287. https://doi.org/10.1007/s10529-009-9984-6
Zohary M (1973) Geobotanical foundations of the Middle East, vol 1. Gustav Fischer Verlag, Stuttgart