The use of ethyl methanesulfonate for the induction of mutants inMycobacterium phlei PA

Folia Microbiologica - Tập 14 - Trang 470-474 - 1969
M. Koníčková-Radochová1, I. Málek1
1Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

Tóm tắt

The mutagenic effect of ethyl methanesulfonate in a concentration of 0.2m on a prototrophic, acid-fast strainMycobacterium phlei PA was studied by following the induction of changes of three genetic markers: prototrophy to auxotrophy and sensitivity to two antituberculosis drugs (INH and STM) to resistence. Ethylmethanesulfonate was found to be a very effective mutagen in all three cases. Thirty auxotrophic strains were obtained, out of which eight exhibited a low frequency of spontaneous reversions and could hence be used for further studies. Of the phenotypes induced the glycine− (serine−) type was most frequently isolated and represented more than half of all auxotrophs obtained. Requirements for lysine and purines were also observed. The EMS treatment (1% survival of the basic suspension) resulted in a 74-fold increase of the frequency of INH-resistant mutants and a frequency of STM-resistant mutants about 1.1/2 to almost 2 orders of magnitude higher than the spontaneous values

Tài liệu tham khảo

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