The use of ethyl methanesulfonate for the induction of mutants inMycobacterium phlei PA
Tóm tắt
The mutagenic effect of ethyl methanesulfonate in a concentration of 0.2m on a prototrophic, acid-fast strainMycobacterium phlei PA was studied by following the induction of changes of three genetic markers: prototrophy to auxotrophy and sensitivity to two antituberculosis drugs (INH and STM) to resistence. Ethylmethanesulfonate was found to be a very effective mutagen in all three cases. Thirty auxotrophic strains were obtained, out of which eight exhibited a low frequency of spontaneous reversions and could hence be used for further studies. Of the phenotypes induced the glycine− (serine−) type was most frequently isolated and represented more than half of all auxotrophs obtained. Requirements for lysine and purines were also observed. The EMS treatment (1% survival of the basic suspension) resulted in a 74-fold increase of the frequency of INH-resistant mutants and a frequency of STM-resistant mutants about 1.1/2 to almost 2 orders of magnitude higher than the spontaneous values
Tài liệu tham khảo
Bautz, E., Freese, E.:On the mutagenic effect of alkylating agents. Proc. nat. Acad. Sci. U. S. 46: 1585, 1960.
Koníčková-Radochová, M., Málek, I.:The mutagenic effect of hydroxylamine on Mycobacterium phlei strain P A. Fol. microbiol. 13: 226, 1968.
Koníčková-Radochová, M., Málek, I.:The mutagenic effect of nitrosoguanidine on Mycobacterium phlei P A. Fol. microbiol. 14: 201, 1969a.
Koníčková-Radochová, M., Málek, I.:The induction of auxotrophic mutants in Mycobacterium phlei P A by ultraviolet radiation. Fol. microbiol. 14: 251, 1969b.
Kreig, D. R.:Ethylmethansulphonate-induced reversions of bacteriophage T4 rII mutants. Genetics 48: 561, 1963.
Lawley, P. D., Brookes, P.:Acidic dissociation of 7: 9-dialkylguanines and its possible relation to mutagenic properties of alkylating agents. Nature 192: 1081, 1961.
Lawley, P. D.:Molecular mechanism of the cytotoxic action of difunctional alkylating agents and of resistance to this action. Nature 206: 480, 1965.
Lindegren, G., Hwang, J. L. Oshima, Y., Lindegren, C. C.:Genetical mutants induced by ethylmethansulfonate in Saccharomyces. Can. J. Genet. Cytol. 7: 491, 1965.
Loprieno, N.:Differential response of Schizosaccharomyces pombe to ethyl methansulfonate and methyl methansulfonate. Mutation Res. 3: 486, 1966.
Loveless, A.:Mutation of T2 bacteriophage as a consequence of alkylation in vitro: the uniqueness of ethylation. Proc. roy. Soc., London B 150: 497, 1959.
Loveless, A., Howarth, S.:Mutation of bacteria at high levels of survival by ethyl methansulfonate. Nature 184: 1780, 1959.
Nečásek, J.:Mutagenic effect of ethyl methanesulfonate in Corynebacterium. The physiology of gene and mutation expression Proceedings of a Symposium held in Prague, 1965, Academia— Prague, 115, 1966.
Straus, B. S.:Specificity of the mutagenic action of the alkylating agents. Nature 191: 730, 1961.
Tessman, I., Poddar, R. K., Kumar, S.:Indentification of the altered bases in mutated singlestranded DNA. I. In vitro mutagenesis by hydroxylamine, ethyl methanesulfonate and nitrous acid. J. mol. Biol. 9: 352, 1964.
Verly, W. G., Barbason, H., Dusart, J., Petipas-Dewandre, A.:A comparative study of the action of ethyl methane sulfonate and HNO 2 on the mutation to streptomycine resistance of Escherichia coli K 12. Biochim. biophys. Acta 145: 752, 1967.