The use of blue-excitable nucleic-acid dyes for the detection of bacteria in well water using a simple field fluorometer and a flow cytometer

Journal of Microbiological Methods - Tập 45 - Trang 143-153 - 2001
Peter J. Stopa1, Sherry A. Mastromanolis1
1The US Army Edgewood Chemical and Biological Center, Engineering Directorate, 5183 Blackhawk Road, AMSSB-REN-E-MC E3549, Aberdeen Proving Ground, MD 21010-5424, USA

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Back, 1991, The differential fluorescence of bacteria stained with acridine orange and the effects of heat, J. Appl. Bacteriol., 71, 51, 10.1111/j.1365-2672.1991.tb04481.x Bartoszcze, 2000, Some problems regarding biological threats, 1 Bruno, 1995, A color image analysis method for assessment of germination based on differential fluorescence staining of bacterial spores and vegetative cells using acridine orange, Biotech. Histochem., 70, 175, 10.3109/10520299509107309 Bruno, 1996, Highly selective acridine and ethidium staining of bacterial DNA and RNA, Biotech. Histochem., 71, 130, 10.3109/10520299609117149 Crissman, 1978, Detailed studies on the application of three fluorescent antibiotics for DNA staining in flow cytometry, Stain Technol., 5, 321, 10.3109/10520297809111954 Darzynkiewicz, 1990, Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange, vol. 33 Davey, 1996, Flow cytometry and cell sorting of heterogenous microbial populations: the importance of single cell analysis, Microbiol. Rev., 60, 641, 10.1128/MR.60.4.641-696.1996 Federal Register, 1996. vol. 61, no. 94.24372. Glazer, 1992, Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection, Nature, 359, 859, 10.1038/359859a0 Guindulain, 1997, Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry, Appl. Environ. Microbiol., 63, 4608, 10.1128/AEM.63.11.4608-4611.1997 Haugland, 1996 Hirons, 1994, TOTO and YOYO; New very bright fluorochromes for DNA content analyses by flow cytometry, Cytometry, 15, 129, 10.1002/cyto.990150206 Kaprylants, 2000, Dormancy in non-sporulating bacteria: its significance for environmental monitoring, 49 Krishna, R.V., Stopa, P.J., 1980. Method to estimate bacteria concentration. Unpublished procedure. Kulaga, 1998, Detection and identification of biological agents by flow cytometric analysis, 95 Lee, 1999, A rapid method for detecting bacteria in drinking water, J. Rapid Methods Autom. Microbiol., 7, 135, 10.1111/j.1745-4581.1999.tb00382.x Lloma, 1999, Realtime warning of biological agent attacks with the Canadian Integrated Bio-Chemical Agent Detectin System II (CIBADS II), Field Anal. Chem. Technol., 3, 260, 10.1002/(SICI)1520-6521(1999)3:4/5<260::AID-FACT5>3.0.CO;2-O Marie, 1996, Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and Pico Green for flow cytometric analysis of marine prokaryotes, Appl. Environ. Microbiol., 63, 4608 McFeters, 1991, Acridine orange staining as an index of physiological activity in Escherichia coli, J. Microbiol. Methods, 13, 87, 10.1016/0167-7012(91)90009-F Millard, 1997, Fluorescence based methods for microbiol characterization and viability assessment, Biotechnol. Int., 1, 291 Monger, 1992, Flow cytometric analysis of marine bacteria with Hoescht 33342, Appl. Environ. Microbiol., 59, 188 Payment, 1991, A randomized trial to evaluate the risk of gastrointestinal disease due to consumption of drinking water meeting current microbiological standards, Am. J. Public Health, 81, 703, 10.2105/AJPH.81.6.703 Porter, 1993, Rapid, automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting, Appl. Environ. Microbiol., 59, 3327, 10.1128/AEM.59.10.3327-3333.1993 Sincock, 2000, The applications of flow cytometry for the detection and characterization of biological aerosols, Field Anal. Chem. Technol., 3, 291 Steen, 1990, DNA measurements of bacteria, Methods Cell Biol., 33, 519, 10.1016/S0091-679X(08)60551-8 Steen, 1982, Applications of flow cytometry on bacteria: cell cycle kinetics, drug effects, and quantitation of antibody binding, Cytometry, 2, 249, 10.1002/cyto.990020409 Steen, 1986, Flow cytometry of bacteria: cell cycle kinetics and effects of antibiotics, Ann. N. Y. Acad. Sci., 486, 329, 10.1111/j.1749-6632.1986.tb42050.x Stopa, P.J., Coon, P.A., Paterno, D.A., 1998. The Biological Detection Kit—a final report. US Army Edgewood Chemical and Biological Center Internal Report. Van Dilla, 1983, Bacterial characterization by flow cytometry, Science, 220, 620, 10.1126/science.6188215