The use of a new grade of polyvinyl alcohol for stabilising tissue sections during histochemical incubations

Springer Science and Business Media LLC - Tập 28 Số 3 - Trang 236-242 - 1971
F. P. Altman1
1Division of Cellular Biology, The Mathilda and Terence Kennedy Institute of Rheumatology, Bute Gardens, Hammersmith, London

Tóm tắt

Từ khóa


Tài liệu tham khảo

Altman, F. P.: The retention and estimation of “soluble” dehydrogenases in unfixed tissue sections. Biochem. J. 96, 53P (1965).

- A comparison of dehydrogenase activities in tissue homogenates and tissue sections. Biochem. J. 114, 13P (1969a).

—: The use of eight different tetrazolium salts for a quantitative study of pentose shunt dehydrogenation. Histochemie 19, 363–374 (1969b).

—: On the oxygen sensitivity of various tetrazolium salts. Histochemie 22, 256–261 (1970).

—: An introduction to the use of tetrazolium salts in quantitative enzyme cytochemistry. Colnbrook, Buckinghamshire, England: Koch-Light Laboratories Ltd. 1972.

—, Chayen, J.: The retention of nitrogenous material in unfixed sections during incubation for histochemical demonstration of enzymes. Nature (Lond.) 207, 1205–1206 (1965).

—: The significance of a functioning hydrogen transport system for the retention of “soluble” dehydrogenases in unfixed sections. J. roy. micr. Soc. 85, 175–180 (1966).

Bitensky, L.: The demonstration of lysosomes by the controlled temperature freezing sectioning method. Quart. J. micr. Sci. 103, 205–209 (1962).

—: The reversible activation of lysosomes in normal cells and the effects of pathological conditions. Ciba Foundation Symposium on Lysosomes, ed. by A. V. S. de Reuck and Margaret P. Cameron, p. 362–375. London: J. and A. Churchill Ltd. 1963.

Butcher, R. G.: Tissue stabilisation during histochemical reaction: the use of collagen polypeptides. Histochemie 28, 231–235 (1971).

Chayen, J., Bitensky, L., Butcher, R. G., Poulter, L. W.: A guide to practical histochemistry. Edinburgh: Oliver & Boyd 1969.

Dahl, H. A., From, S. H.: Some effects of polyvinyl alcohol and polyvinyl pyrrolidone on the activity of lactate dehydrogenase and its isoenzymes. Histochemie 25, 182–190 (1971).

—, Mellgren, S. I.: The effect of polyvinyl alcohol and polyvinyl pyrrolidone on diffusion artifacts in lactate dehydrogenase histochemistry. Histochemie 24, 354–370 (1970).

Farber, E., Sternberg, W. H., Dunlap, C. E.: Histochemical localisation of specific oxidative enzymes. I. Tetrazolium stains for diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase. J. Histochem. Cytochem. 4, 254–265 (1956).

Jacobsen, N. O.: The histochemical localization of lactic dehydrogenase isoenzymes in the rat nephron by means of an improved polyvinyl alcohol method. Histochemie 20, 250–265(1969).

Jones, G. R. N.: Losses of nitrogenous material occurring from frozen sections of rat liver during incubation in an aqueous medium. Biochem. J. 96, 10P (1965).

Kalina, M., Gahan, P. B.: A quantitative study of the validity of the histochemical demonstration for pyridine nucleotide-linked dehydrogenases. Histochemie 5, 430–436 (1965).

—: A gelatin film method for improved histochemical localization of dehydrogenases in plant cells. Stain Technol. 43, 51–57 (1968).

Kunze, K. D.: Beitrag zur Histochemie der Laktatdehydrogenase. Histochemie 11, 350–359 (1967).

Nachlas, M. M., Prinn, W., Seligman, A. M.: Quantitative estimation of lyo- and desmo enzymes in tissue sections with and without fixation. J. biophys. biochem. Cytol. 2, 487–502 (1956).

Novikoff, A. B.: Microscopy and differential centrifugation, p. 315–317. In: Proc. Third Int. Congr. Biochem., Brussels 1955 (C. Liebecq, ed.). 544 p. New York: Academic Press 1956.

—: Biochemical heterogeneity of the cytoplasmic particles of rat liver. Symp. Soc. exp. Biol. 10, 92–109 (1957),

—: Electron transport enzymes: Biochemical and tetrazolium staining studies, p. 465–481. In: First Int. Congr. Histochem. Cytochem. Oxford-London-New York-Paris: Pergamon Press 1963.

— Arase, M. M.: The intracellular localisation of lactic dehydrogenase studied by biochemical and staining methods. J. Histochem. Cytochem. 6, 251–254 (1958).

Scarpelli, D. G., Pearse, A. G. E.: Physical and chemical protection of cell constituents and the precise localization of enzymes. J. Histochem. Cytochem. 6, 369–376 (1958).

Sloane-Stanley, G. H.: A simple procedure for the estimation of very small amounts of nitrogen in lipids. Biochem. J. 104, 293–295 (1967).

Stuart, J., Skowron, P. N.: A cytochemical study of marrow enzymes in megaloblastic anaemia. Brit. J. Haemat. 15, 443–454 (1968).

Walker, D. G., Seligman, A. M.: The use of formalin fixation in the cytochemical demonstration of succinic and DPN and TPN-dependent dehydrogenases in mitochondria. J. Cell Biol. 16, 455–469 (1963).

Wenk, H.: Beitrag zum histochemischen Nachweis löslicher NAD-abhängiger Dehydrogenasen mittels PVA-Gelinkubation. Acta histochem. (Jena) 35, 380–391 (1970).