The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomes

Desquesnes M, Davila AM (2002) Applications of PCR-based tools for detection and identification of animal trypanosomes: a review and perspectives. Vet Parasitol 11:213?231

Desquesnes M, McLaughlin G, Zoungrana A, Davila AM (2001) Detection and identification of Trypanosoma of African livestock through a single PCR based on internal transcribed spacer 1 of rDNA. Int J Parasitol 31:610?614

Gibson WC, Stevens JR, Mwendia CMT, Makumi JM, Ngotho JM, Ndung?u JM (2001) Unraveling the phylogenetic relationships of African trypanosomes of suids. Parasitology 122:625?631

Hernandez P, Martin-Parras L, Martinez-Robles ML, Schvartzman JB (1993) Conserved features in the mode of replication of eukaryotic ribosomal RNA genes. EMBO J 12:1475?1485

Lehane MJ, Msangi AR, Whitaker CJ, Lehane SM (2000) Grouping of trypanosomes species in mixed infections in Glossina pallidipes. Parasitology 120:583?592

Majiwa PAO, Thatti R, Moloo SK, Nyeko JHP, Otieno LH, Moloo S (1994) Detection of trypanosome infections in the saliva of tsetse flies and buffy coat samples from antigenic but aparasitaemic cattle. Parasitology 108:313?322

Malele I, Craske L, Knight C, Ferris V, Njiru ZK, Hamilton P, Stella L, Lehane M, Gibson WC (2003) The use of specific and generic primers to identify trypanosome infections of wild tsetse flies in Tanzania by PCR. Infect Genet Evol 3:271?279

Masake RA, Majiwa PAO, Moloo SK, Makau JM, Njuguna JT, Maina M, Kabata J, Ole-MoiYoi, OK, Nantulya VM (1997) Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction. Exp Parasitol 85:193?205

Masiga DK, Smyth AJ, Hayes P, Bromidge TJ, Gibson WC (1992) Sensitive detection of trypanosomes in tsetse flies by DNA amplification. Int J Parasitol 22:909?918

McLaughlin Gl, Ssenyonga SS, Nanteza E, Rubaire-Akiki, Wafula O, Hansen RD, Vodkin MH, Novak RJ, Gordon VR, Montenegro-James S, James M, Aviles H, Armijos R, Santrich C, Weigle K, Saravia N, Wozniak E, Gaye O, Mdachi R, Shapiro SZ, Chang KP, Kakoma I (1996) PCR based detection and typing of parasites. In: Zcel MA, Alkan MZ (eds) Parasitology for the 20th century. CAB International, Wallingford, pp 261?287

Morlais I, Ravel S, Grebaut P, Dumas V, Cuny G (2001) New molecular marker for Trypanosoma (Duttonella) vivax identification. Acta Trop 80:207?213

Mugittu KN, Silayo RS, Majiwa PAO, Kimbita EK, Mutayoba BM, Maselle R (2000) Application of PCR and DNA probes in the characterization of trypanosomes in the blood of cattle in farms in Morogoro Tanzania. Vet Parasitol 94:177?189

Murray M, Murray PK, McIntyre IM (1977) An improved parasitological technique for the diagnosis of African trypanosomiasis. Trans R Soc Trop Med Hyg 71:325?326

Njiru ZK, Makumi JN, Okoth S, Ndungu JM, Gibson WC (2004) Identification of trypanosomes in Glossina pallidipes and G. longipennis in Kenya. Infect Genet Evol 4:29?35

Stevens J, Rambaut A (2001) Evolutionary rate differences in trypanosomes. Infect Genet Evol 1:143?150

Solano P, Michel JF, Lefrancois T, De La Rocque S, Sidibe I, Zoungrana A, Cuisance D (1999) Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso. Vet Parasitol 30:95?103

Woo PTR (1970) The haematocrit centrifuge technique for the diagnosis of African trypanosomes. Acta Trop 27:384?386

Wuyts N, Chokesajjawatee N, Panyim S (1994) A simplified and highly sensitive detection of Trypanosoma evansi by DNA amplification. S E Asian J Trop Med Public Health 25:266?271