The transcriptome of retinal Müller glial cells

Journal of Comparative Neurology - Tập 509 Số 2 - Trang 225-238 - 2008
Karin Roesch1, Ashutosh P. Jadhav1, Jeffrey M. Trimarchi1, Michael Stadler2, Botond Roska2, Ben B. Sun3,1, Constance L. Cepko1,4
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115
2Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
3Canaccord Adams, Boston, Massachusetts 02110
4Howard Hughes Medical Institute, Boston, Massachusetts 02115

Tóm tắt

AbstractMüller glial cells are the major type of glia in the mammalian retina. To identify the molecular machinery that defines Müller glial cell identity and function, single cell gene expression profiling was performed on Affymetrix microarrays. Identification of a cluster of genes expressed at high levels suggests a Müller glia core transcriptome, which likely underlies many of the functions of Müller glia. Expression of components of the cell cycle machinery and the Notch pathway, as well as of growth factors, chemokines, and lipoproteins might allow communication between Müller glial cells and the neurons that they support, including modulation of neuronal activity. This approach revealed a set of transcripts that were not previously characterized in (Müller) glia; validation of the expression of some of these genes was performed by in situ hybridization. Genes expressed exclusively by Müller glia were identified as novel markers. In addition, a novel BAC transgenic mouse that expresses Cre in Müller glia cells was generated. The molecular fingerprint of Müller glia provides a foundation for further studies of Müller glia development and function in normal and diseased states. J. Comp. Neurol. 509:225–238, 2008. © 2008 Wiley‐Liss, Inc.

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Tài liệu tham khảo

10.1074/jbc.271.10.5628

10.1002/cne.1300

10.1016/j.neuron.2006.04.027

Bao ZZ, 1997, The expression and function of Notch pathway genes in the developing rat eye, J Neurosci, 15, 1425

10.1073/pnas.0402140101

10.1016/S0092-8674(01)00574-8

10.1371/journal.pbio.0020247

10.1016/0076-6879(93)25039-5

10.1002/1097-0029(20000901)50:5<384::AID-JEMT7>3.0.CO;2-W

10.1242/dev.127.14.3021

10.1111/j.1469-7580.2006.00671.x

10.1016/1350-9462(94)90015-9

Che S, 2004, Amplification of RNA transcripts using terminal continuation, Lab Invest, 84, 131, 10.1038/labinvest.3700005

10.1016/S0925-4773(99)00251-8

10.1523/JNEUROSCI.23-05-01730.2003

10.1523/JNEUROSCI.16-16-05082.1996

10.1002/cne.10674

10.1016/0092-8674(95)90161-2

10.1038/78774

10.1073/pnas.95.25.14863

10.1016/j.neuron.2006.03.014

10.1038/85090

10.1002/glia.10218

10.1023/A:1011987107034

10.1073/pnas.0611180104

10.1016/S0896-6273(00)81171-X

10.1146/annurev.neuro.25.030702.130823

10.1038/ng1192-232

10.1167/iovs.05-1570

10.1038/354522a0

10.1242/dev.127.12.2515

10.1016/0006-8993(94)91072-3

Inoue T, 2002, Math3 and NeuroD regulate amacrine cell fate specification in the retina, Development, 129, 831, 10.1242/dev.129.4.831

10.1038/nbt729

10.1016/j.febslet.2005.12.017

10.1073/pnas.0608155103

10.1242/dev.02245

10.1016/0968-0004(92)90325-4

10.1038/nn1620

10.1006/dbio.2001.0157

Laskowitz DT, 2000, Altered immune responses in apolipoprotein E deficient mice, J Lipid Res, 41, 613, 10.1016/S0022-2275(20)32409-3

10.1006/geno.2000.6451

10.5483/BMBRep.2007.40.2.226

10.1101/gr.749203

10.1073/pnas.0307014101

10.1016/j.neuron.2006.04.028

10.1126/science.3283935

Mano T, 1990, Phagocytosis by human retinal glial cells in culture, Invest Ophthalmol Vis Sci, 31, 1047

10.1016/S0092-8674(01)00295-1

10.3109/08977199109000260

10.1016/S0896-6273(02)00912-1

10.1073/pnas.2235688100

10.1016/j.cub.2003.12.058

10.1167/iovs.02-0260

10.1016/S0092-8674(00)80860-0

10.1242/dev.126.1.23

10.1101/gad.13.2.225

10.1523/JNEUROSCI.18-07-02506.1998

Nakazawa T, 2006, Characterization of cytokine responses to retinal detachment in rats, Mol Vis, 12, 867

10.1073/pnas.0608167104

10.1016/0166-2236(96)10040-0

10.1074/jbc.M102420200

10.1073/pnas.0402129101

10.1523/JNEUROSCI.4193-06.2007

10.1002/path.1645

10.1016/S0021-9258(18)47945-8

10.1007/978-94-011-0533-0_16

10.1167/iovs.06-0677

10.1016/S0166-2236(97)01139-9

10.1016/j.ydbio.2004.03.039

Sahel JA, 1990, Proliferation of retinal glia and excitatory amino acids, Ophtalmologie, 4, 13

Seigel GM, 1996, Expression of glial markers in retinal precursor cell line, Mol Vis, 24, 22

Sidman RL, 1961, The structure of the eye, 487

Sinohara H, 2004, Gi2 signaling enhances proliferation of neural progenitor cells in the developing brain, J Biol Chem, 279, 41141, 10.1074/jbc.M406721200

Stolzenburg JU, 1992, Phagocytosis of latex beads by rabbit retinal Müller (glial) cells in vitro, J Hirnforsch, 33, 557

10.1002/cne.902550104

10.1186/gb-2006-7-3-r18

10.1016/S0896-6273(03)00229-0

10.1002/cne.21368

10.1038/328131a0

10.1016/0896-6273(90)90136-4

10.1073/pnas.87.5.1663

10.1002/glia.20195

10.1152/physiolgenomics.2000.2.3.143

10.1038/332834a0

10.1679/aohc.65.27

10.1002/ar.1092120215

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Journal of Comparative Neurology

Tập 509 Số 2

225-238

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