The target gene of tae‐miR164, a novel NAC transcription factor from the NAM subfamily, negatively regulates resistance of wheat to stripe rust

Molecular Plant Pathology - Tập 15 Số 3 - Trang 284-296 - 2014
Hao Feng1, Xiaoyuan Duan2, Qiong Zhang2, Xiaorui Li1, Bing Wang1, Lili Huang1, Xiaojie Wang1, Zhensheng Kang1
1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 China
2College of Life Sciences Northwest A&F University Yangling Shaanxi 712100 China

Tóm tắt

SummarymicroRNA (miRNA) participates in various physiological and biochemical processes in plants by regulating corresponding target genes. NAC [NAM (no apical meristem), ATAF (Arabidopsis transcription activation factor) and CUC (cup‐shaped cotyledon)] transcription factors, usually as the targets of miR164, play important roles in the regulation of plant development and responses to abiotic and biotic stresses. In a previous study, the target gene of tae‐miR164 in wheat was sequenced through degradome sequencing. In this study, we isolated the full‐length cDNA of the candidate target gene, which is a NAC transcription factor gene in the NAM subfamily, and designated it as TaNAC21/22 after bioinformatics analysis. The interaction between TaNAC21/22 and tae‐miR164 was confirmed experimentally through co‐transformation of both genes in tobacco leaves. Transcript accumulation of TaNAC21/22 and tae‐miR164 showed contrasting divergent expression patterns in wheat response to Puccinia striiformis f. sp. tritici (Pst). TaNAC21/22 was confirmed to be located in the nucleus and could function as a transcriptional activator. Silencing of the individual gene showed that TaNAC21/22 negatively regulates resistance to stripe rust. These results indicate that the target of tae‐miR164, a novel NAC transcription factor from the NAM subfamily of wheat, plays an important role in regulating the resistance of host plants to stripe rust.

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Molecular Plant Pathology

Tập 15 Số 3

284-296

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