The shift in GH3 cell shape and cell motility is dependent on MLCK and ROCK

Mollard, 2012, A tridimensional view of pituitary development and function, Trends Endocrinol. Metab., 23, 261, 10.1016/j.tem.2012.02.004 Saeger, 2007, Pathohistological classification of pituitary tumors: 10 years of experience with the German pituitary tumor registry, Eur. J. Endocrinol., 156, 203, 10.1530/eje.1.02326 Yamada, 2007, A study of the correlation between morphological findings and biological activities in clinically nonfunctioning pituitary adenomas, Neurosurgery, 61, 580, 10.1227/01.NEU.0000290906.53685.79 Di Ieva, 2014, Aggressive pituitary adenomas-diagnosis and emerging treatments, Nat. Rev. Endocrinol., 10, 423, 10.1038/nrendo.2014.64 Vicente-Manzanares, 2005, Cell migration at a glance, J. Cell Sci., 118, 4917, 10.1242/jcs.02662 Friedl, 2010, Plasticity of cell migration: a multiscale tuning model, J. Cell Biol., 188, 11, 10.1083/jcb.200909003 Hynes, 2009, The extracellular matrix: not just pretty fibrils, Science, 326, 1216, 10.1126/science.1176009 Azorín, 2014, GH3 tumor pituitary cell cytoskeleton and plasma membrane arrangement are determined by extracellular matrix proteins: implications on motility, proliferation and hormone secretion, Int. J. Physiol. Pathophysiol. Pharmacol., 6, 66 Wakatsuki, 2003, Mechanics of cell spreading: role of myosin II, J. Cell Sci., 116, 1617, 10.1242/jcs.00340 Schwarz, 2012, United we stand: integrating the actin cytoskeleton and cell-matrix adhesions in cellular mechanotransduction, J. Cell Sci., 125, 3051, 10.1242/jcs.093716 Paluch, 2013, The role and regulation of blebs in cell migration, Curr. Opin. Cell Biol., 25, 582, 10.1016/j.ceb.2013.05.005 Salbreux, 2012, Actin cortex mechanics and cellular morphogenesis, Trends Cell Biol., 22, 536, 10.1016/j.tcb.2012.07.001 Hartshorne, 1998, Myosin light chain phosphatase: subunit composition, interactions and regulation, J. Muscle Res. Cell Motil., 19, 325, 10.1023/A:1005385302064 Kamm, 2001, Dedicated myosin light chain kinases with diverse cellular functions, J. Biol. Chem., 276, 4527, 10.1074/jbc.R000028200 Somlyo, 2003, Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase, Physiol. Rev., 83, 1325, 10.1152/physrev.00023.2003 Hong, 2011, Biochemistry of smooth muscle myosin light chain kinase, Arch. Biochem. Biophys., 510, 135, 10.1016/j.abb.2011.04.018 Fukata, 2001, Rho-family GTPases in cadherin-mediated cell-cell adhesion, Nat. Rev. Mol. Cell Biol., 2, 887, 10.1038/35103068 Totsukawa, 2004, Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts, J. Cell Biol., 164, 427, 10.1083/jcb.200306172 Senju, 2009, The role of actomyosin contractility in the formation and dynamics of actin bundles during fibroblast spreading, J. Biochem., 145, 137, 10.1093/jb/mvn151 Katoh, 2011, Rho-associated kinase-dependent contraction of stress fibres and the organization of focal adhesions, J. R. Soc. Interface, 8, 305, 10.1098/rsif.2010.0419 Hall, 2000, Rho GTPases: molecular switches that control the organization and dynamics of the actin cytoskeleton, Philos. Trans. R. Soc. Lond. B Biol. Sci., 355, 965, 10.1098/rstb.2000.0632 Nobes, 1999, Rho GTPases control polarity, protrusion, and adhesion during cell movement, J. Cell Biol., 144, 1235, 10.1083/jcb.144.6.1235 Ridley, 1992, The small GTP-binding protein Rho regulates the assembly of focal adhesions and stress fibers in response to growth factors, Cell, 70, 389, 10.1016/0092-8674(92)90163-7 Smith, 2003, LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment, J. Cell Sci., 116, 3123, 10.1242/jcs.00606 Sarkar, 2009, Insights into the roles of non-muscle myosin iia in human keratinocyte migration, Cell Mol. Bioeng., 2, 486, 10.1007/s12195-009-0094-2 Lou, 2015, Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase, J. Cell Biol., 209, 275, 10.1083/jcb.201409001 Chew, 2002, A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows, J. Cell Biol., 156, 543, 10.1083/jcb.200110161 Katoh, 2001, Stress fiber organization regulated by MLCK and Rho-kinase in cultured human fibroblast, Am. J. Cell Physiol., 280, 1669, 10.1152/ajpcell.2001.280.6.C1669 Wong, 2006, Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism, Proc. Natl. Acad. Sci. USA, 103, 3639, 10.1073/pnas.0600092103 Xu, 2008, Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating β2 integrins, Nat. Immunol., 9, 880, 10.1038/ni.1628 Mansfield, 2000, Regulation of polymorphonuclear leukocyte phagocytosis by myosin light chain kinase after activation of mitogen-activated protein kinase, Blood, 95, 2407, 10.1182/blood.V95.7.2407 Ishizaki, 2000, Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases, Mol. Pharmacol., 57, 976 Nguyen, 1999, Myosin light chain kinase functions downstream of Ras/ERK to promote migration of urokinase-type plasminogen activator-stimulated cells in an integrin-selective manner, J. Cell Biol., 146, 149, 10.1083/jcb.146.1.149 Huveneers, 2012, Vinculin associates with endothelial VE-cadherin junctions to control force-dependent remodeling, J. Cell Biol., 196, 641, 10.1083/jcb.201108120 Gao, 2004, Rational design and characterization of a Rac GTPase-specific small molecule inhibitor, Proc. Natl. Acad. Sci. USA, 101, 7618, 10.1073/pnas.0307512101 Surviladze, 2010 Sakurada, 1998, Dynamics of myosin light chain phosphorylation at Ser19 and Thr18/Ser19 in smooth muscle cells in culture, Am. J. Physiol., 274, 1563, 10.1152/ajpcell.1998.274.6.C1563 Sheetz, 2001, Cell control by membrane-cytoskeleton adhesion, Nat. Rev. Mol. Cell Biol., 2, 392, 10.1038/35073095 Diz-Muñoz, 2013, Use the force: membrane tension as an organizer of cell shape and motility, Trends Cell Biol., 23, 47, 10.1016/j.tcb.2012.09.006 Ikebe, 1987, Phosphorylation of the 20,000-Da light chain of smooth muscle myosin by the calcium-activated, phospholipid-dependent protein kinase, J. Biol. Chem., 262, 9569, 10.1016/S0021-9258(18)47971-9 Itoh, 1999, An essential part for Rho-associated kinase in the transcellular invasion of tumor cells, Nat. Med., 5, 221, 10.1038/5587 Small, 1998, Assembling an actin cytoskeleton for cell attachment and movement, Biochim. Biophys. Acta, 1404, 271, 10.1016/S0167-4889(98)00080-9 Toral, 2007, Role of extracellular matrix-cell interaction and epidermal growth factor (EGF) on EGF-receptors and actin cytoskeleton arrangement in infantile pituitary cells, Cell Tissue Res., 327, 143, 10.1007/s00441-006-0248-7 Avila-Rodríguez, 2016, Three-dimensional alginate-bead culture of human pituitary adenoma cells, J. Vis. Exp., 18, 53637 Gauthier, 2012, Mechanical feedback between membrane tension and dynamics, Trends Cell Biol., 22, 527, 10.1016/j.tcb.2012.07.005 Bergert, 2012, Cell mechanics control rapid transitions between blebs and lamellipodia during migration, Proc. Natl. Acad. Sci. USA, 109, 14434, 10.1073/pnas.1207968109 Tinevez, 2009, Role of cortical tension in bleb growth, Proc. Natl. Acad. Sci. USA, 106, 18581, 10.1073/pnas.0903353106 Sahai, 2003, Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis, Nat. Cell Biol., 5, 711, 10.1038/ncb1019 Charras, 2006, Reassembly of contractile actin cortex in cell blebs, J. Cell Biol., 175, 477, 10.1083/jcb.200602085 Sandquist, 2006, Rho kinase differentially regulates phosphorylation of nonmuscle myosin II isoforms A and B during cell rounding and migration, J. Biol. Chem., 281, 35873, 10.1074/jbc.M605343200 Gadea, 2007, Loss of p53 promotes RhoA-ROCK-dependent cell migration and invasion in 3D matrices, J. Cell Biol., 178, 23, 10.1083/jcb.200701120 Raucher, 2000, Cell spreading and lamellipodial extension rate is regulated by membrane tension, J. Cell Biol., 148, 127, 10.1083/jcb.148.1.127 Getz, 2010, Differential phosphorylation of myosin light chain (Thr)18 and (Ser)19 and functional implications in platelets, J. Thromb. Haemost., 8, 2283, 10.1111/j.1538-7836.2010.04000.x Ridley, 2003, Cell migration: integrating signals from front to back, Science, 302, 1704, 10.1126/science.1092053 Keren, 2011, Cell motility: the integrating role of the plasma membrane, Eur. Biophys. J., 40, 1013, 10.1007/s00249-011-0741-0 Geiger, 2002, Exploring the neighborhood: adhesion-coupled cell mechanosensors, Cell, 110, 139, 10.1016/S0092-8674(02)00831-0 Choi, 2008, Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner, Nat. Cell Biol., 10, 1039, 10.1038/ncb1763 Alexandrova, 2008, Evolution of cell interactions with extracellular matrix during carcinogenesis, Biochemistry, 73, 733 Nakamura, 2013, FilGAP and its close relatives: a mediator of Rho-Rac antagonism that regulates cell morphology and migration, Biochem. J., 453, 17, 10.1042/BJ20130290 Mogilner, 2009, The shape of motile cells, Curr. Biol., 19, 762, 10.1016/j.cub.2009.06.053 Verkhovsky, 2015, The mechanisms of spatial and temporal patterning of cell-edge dynamics, Curr. Opin. Cell Biol., 36, 113, 10.1016/j.ceb.2015.09.001 Sanz-Moreno, 2010, The plasticity of cytoskeletal dynamics underlying neoplastic cell migration, Curr. Opin. Cell Biol., 22, 690, 10.1016/j.ceb.2010.08.020 Ridley, 2015, Rho GTPase signalling in cell migration, Curr. Opin. Cell Biol., 36, 103, 10.1016/j.ceb.2015.08.005 Leung, 1998, Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization, Mol. Cell. Biol., 18, 130, 10.1128/MCB.18.1.130 Zhuoshen, 2015, Myotonic dystrophy kinase-related Cdc42-binding kinases (MRCK), the ROCK-like effectors of Cdc42 and Rac1, Small GTPases, 6, 81, 10.1080/21541248.2014.1000699