The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines

Toxicology - Tập 124 Số 3 - Trang 179-192 - 1997
Gregor Malich1, Boban Markovic2, Chris Winder2
1Department of Safety Science, University of New South Wales, Sydney, Australia.
2Chemical Safety and Applied Toxicology Laboratories, Department of Safety Science, University of New South Wales, Sydney 2052, Australia.

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Agrawal, 1996, Frequency and occurrence of LD50 values for materials in the workplace, J. Appl. Toxicol., 16, 407, 10.1002/(SICI)1099-1263(199609)16:5<407::AID-JAT365>3.0.CO;2-V

Atterwill, C.K. 1995. Alternative method of assessing toxicity. In: O'Hare, S., Attenwill, C.K. (Eds.), Methods in Molecular Biology—volume 43: In Vitro Toxicity Testing Protocols. Humana Press, Totowa, pp. 1–9.

Balls, 1992, The use of basal cytotoxicity and target organ toxicity tests in hazard identification and risk assessment, Altern. Lab. Animals (ATLA), 20, 368, 10.1177/026119299202000304

Barile, 1994, In vitro cytotoxicity testing for prediction of acute human toxicity, Cell Biol. Toxicol., 10, 155, 10.1007/BF00757558

Barltrop, 1991, 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl)tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators, Bioorg. Med. Chem. Lett., 1, 611, 10.1016/S0960-894X(01)81162-8

Berg, 1994, The use of a watersoluble formazan complex to quantitate the cell number and mitochondrial function of Leishmania major promastigotes, Parasitol. Res., 80, 235, 10.1007/BF00932680

Bitton, 1992, Bacterial and enzymatic bioassays for toxicity testing in the environment, Rev. Environ. Contam. Toxicol., 125, 1

Bondesson, 1989, MEIC—a new international multicenter project to evaluate the relevance to human toxicity of in vitro cytotoxicity tests, Cell Biol. Toxicol., 5, 331, 10.1007/BF01795360

Buttke, 1993, Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines, J. Immunol. Methods, 157, 233, 10.1016/0022-1759(93)90092-L

Cairns, 1990, Biological monitoring of hazardous waste sites, Comments Toxicol., 3, 493

Calleja, 1993, The predictive potential of a battery of ecotoxicological tests for human acute toxicity, as evaluated with the first 50 MEIC chemicals, Altern. Lab. Animals (ATLA), 21, 330, 10.1177/026119299302100304

Clemedson, C., McFarlane-Abdulla, E., Andersson, M., Barile, F.A., Calleja, M.C., Chesné, C., Clothier, R., Cottin, M., Curren, R., Daniel-Szolgay, E., Dierickx, P., Ferro, M., Fiskesjö, G., Garza-Ocañas, L., Gómez-Lechón, M.J., Gülden, M., Isomaa, B., Janus, J., Judge, P., Kahru, A., Kemp, R.B., Kerszman, G., Kristen, U., Kunimoto, M., Kärenlampi, S., Lavrijsen, K., Lewan, L., Lilius, H., Malmsten, A., Ohno, T., Persoone, G., Roguet, R., Romert, L., Sawyer, T.W., Seibert, H., Shrivastava, R., Stammati, A., Tanaka, N., Torres-Alanis, O., Voss, J.U., Wakuri, S., Walum, E., Wang, X., Zucco, F., Ekwall, B., 1996. MEIC evaluation of acute systemic toxicity—part 1: methodology of 68 in vitro toxicity assays used to test the first 30 reference chemicals. Altern. Lab. Animals (ATLA) 24 (1), 251–272.

Clemedson, C., McFarlane-Abdulla, E., Andersson, M., Barile, F. A., Calleja, M.C., Chesné, C., Clothier, R., Cottin, M., Curren, R., Dierickx, P., Ferro, M., Fiskesjö, G., Garza-Ocañas, L., Gómez-Lechón, M.J., Gülden, M., Isomaa, B., Janus, J., Judge, P., Kahru, A., Kemp, R.B., Kerszman, G., Kristen, U., Kunimoto, M., Kärenlampi, S., Lavrijsen, K., Lewan, L., Lilius, H., Malmsten, A., Ohno, T., Persoone, G., Pettersson, R., Roguet, R., Romert, L., Sandberg, M., Sawyer, T.W., Seibert, H., Shrivastava, R., Sjöström, M., Stammati, A., Tanaka, N., Torres-Alanis, O., Voss, J.U., Wakuri, S., Walum, E., Wang, X., Zucco, F., Ekwall, B., 1996. MEIC evaluation of acute systemic toxicity—part II: in vitro results from 68 toxicity assays used to test the first 30 reference chemicals and a comparative cytotoxicity analysis. Altern. Lab. Animals (ATLA) 24 (1), 273–311.

Cory, 1991, Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture, Cancer Commun., 3, 207, 10.3727/095535491820873191

Ekwall, 1980, Toxicity to HeLa cells of 205 drugs as determined by the metabolic inhibition test supplemented by microscopy, Toxicology, 17, 273, 10.1016/0300-483X(80)90010-4

Ekwall, 1983, Correlation between cytotoxicity in vitro and LD50 values, Pharmacol. Toxicol., 52, 80

Ekwall, B., 1995. The basal cytotoxicity concept. In: Goldberg, A.M., Van Zutphen, L.F.M. (Eds.), The World Congress on Alternatives and Animal Use in the Life Sciences: Education, Research, Testing. Mary Ann Liebert Publishers, New York, pp. 721–725.

Faustman, E.M., Omenn, G.S., 1996. Risk assessment. In: Klaassen, C.D. (Ed.), Casarett and Doull's toxicology—the basic science of poisons, 5th ed. McGraw-Hill, New York, pp. 75–88.

Goodwin, 1995, Microculture tetrazolium assays: a comparison between two new tetrazolium salts XTT and MTS, J. Immunol. Methods, 179, 95, 10.1016/0022-1759(94)00277-4

Lambolez, 1994, The environmental risks of industrial waste disposal: an experimental approach including acute and chronic toxicity studies, Ecotoxicol. Environ. Safety, 28, 317, 10.1006/eesa.1994.1056

Marinovich, 1996, Effect of pesticide mixtures on in vitro nervous cells: comparison with single pesticides, Toxicology, 108, 201, 10.1016/0300-483X(96)03303-3

Potier, 1995, Interest and limits of human tissue and cell use in pharmacotoxicology, Cell Biol. Toxicol., 11, 133, 10.1007/BF00756514

Promega, 1996. CellTiter 96™ AQueous non-radioactive cell proliferation assay. Technical Bulletin 169, revised, Promega Corporation, Madison.

Riss, 1992, Comparison of MTT, XTT and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays, Mol. Biol. Cell, 3, 184a

Riss, T.L., Moravec, R.A., 1996. Improved non-radioactive assay to measure cellular proliferation or toxicity: the CellTiter 96® AQueous One Solution cell proliferation assay. Promega Notes 59, Promega, Madison, pp. 19–23.

Rotter, 1993, Rapid colorimetric bioassay for screening of Fusarium mycotoxins, Nat. Toxins, 1, 303, 10.1002/nt.2620010509

Shrivastava, 1992, Comparison of in vivo acute lethal potency and in vitro cytotoxicity of 48 chemicals, Cell Biol. Toxicol., 8, 157, 10.1007/BF00260565

Weiss, 1993, Cytotoxicity of the MEIC test chemicals in primary neurone cultures, Toxicol. in Vitro, 7, 653, 10.1016/0887-2333(93)90100-J