The role of human umbilical cord mesenchymal stem cells-derived exosomal microRNA-431-5p in survival and prognosis of colorectal cancer patients

Mutagenesis - Tập 37 Số 2 - Trang 164-171 - 2022
Muwen Qu1, Junyi Li2, Hong Zi-fu1, Fei Jia1, Jun Li1, Lingling Yuan3
1Anorectal Department of Guang’anmen Hospital of Chinese Academy of Chinese Medical Sciences, No. 5, Beixiange, Xicheng District, 100053 Beijing, China
2Surgical Department of Guang’anmen Hospital of Chinese Academy of Chinese Medical Sciences, No. 5, Beixiange, Xicheng District, 100053 Beijing, China
3Department of Dermatology, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, No. 5, Haiyuncang, Dongcheng District, 100700 Beijing, China

Tóm tắt

Abstract We aim to discuss the role of miR-431-5p in colorectal cancer (CRC) progression via regulating peroxiredoxin 1 (PRDX1). miR-431-5p and PRDX1 expression were detected in CRC tissues and cells, and the relationship between miR-431-5p expression and prognosis of CRC patients was analyzed. Exosomes were extracted from human umbilical cord mesenchymal stem cells (hUCMSCs) and co-cultured with LoVo cells. MTT assay, flow cytometry and Transwell assay were implemented to test cell viability, apoptosis and invasion and migration ability, respectively. The tumor growth was determined as well, and the binding relation between miR-431-5p and PRDX1 was confirmed. miR-431-5p was downregulated and PRDX1 was upregulated in CRC, and miR-431-5p downregulation was associated with poor prognosis. hUCMSC-Exos suppressed the malignant behaviors of LoVo cells, and overexpression of miR-431-5p further aggravated the inhibitory effect of hUCMSC-Exos on LoVo cells. hUCMSC-Exos inhibited PRDX1 expression via miR-431-5p. PRDX1 was targeted by miR-431-5p. miR-431-5p serves as a prognostic biomarker in CRC, and hUCMSC-Exos transfer of miR-431-5p decelerates CRC cell growth by inhibiting PRDX1.

Từ khóa


Tài liệu tham khảo

Hong, 2019, Current status of nanoscale drug delivery systems for colorectal cancer liver metastasis., Biomed Pharmacother, 114, 108764, 10.1016/j.biopha.2019.108764

Aran, 2016, Colorectal cancer: epidemiology, disease mechanisms and interventions to reduce onset and mortality., Clin Colorectal Cancer, 15, 195, 10.1016/j.clcc.2016.02.008

Afrăsânie, 2019, KRAS, NRAS, BRAF, HER2 and microsatellite instability in metastatic colorectal cancer—practical implications for the clinician., Radiol Oncol, 53, 265, 10.2478/raon-2019-0033

Kamarudin, 2019, Metformin in colorectal cancer: molecular mechanism, preclinical and clinical aspects., J Exp Clin Cancer Res, 38, 491, 10.1186/s13046-019-1495-2

Li, 2018, Cardiac progenitor cell-derived exosomes promote H9C2 cell growth via Akt/mTOR activation., Int J Mol Med, 42, 1517

Liu, 2019, Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis., J Physiol Biochem, 75, 391, 10.1007/s13105-019-00692-6

Hu, 2020, Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism., Theranostics, 10, 2293, 10.7150/thno.39238

You, 2019, Snail1-dependent cancer-associated fibroblasts induce epithelial-mesenchymal transition in lung cancer cells via exosomes., QJM, 112, 581, 10.1093/qjmed/hcz093

Zhao, 2020, miR-33-5p knockdown attenuates abdominal aortic aneurysm progression via promoting target adenosine triphosphate-binding cassette transporter A1 expression and activating the PI3K/Akt signaling pathway., Perfusion, 35, 57, 10.1177/0267659119850685

Huang, 2019, miR-4319 suppresses colorectal cancer progression by targeting ABTB1., United European Gastroenterol J, 7, 517, 10.1177/2050640619837440

Fu, 2019, miR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1., Cell Mol Biol Lett, 24, 31, 10.1186/s11658-019-0155-z

Jiang, 2019, FBXL19-AS1 exerts oncogenic function by sponging miR-431-5p to regulate RAF1 expression in lung cancer, Biosci Rep, 39, 1, 10.1042/BSR20181804

Liu, 2019, PRDX1 enhances cerebral ischemia-reperfusion injury through activation of TLR4-regulated inflammation and apoptosis., Biochem Biophys Res Commun, 519, 453, 10.1016/j.bbrc.2019.08.077

Li, 2018, Peroxiredoxin 1 promoted tumor metastasis and angiogenesis in colorectal cancer., Pathol Res Pract, 214, 655, 10.1016/j.prp.2018.03.026

Chu, 2016, Identification and verification of PRDX1 as an inflammation marker for colorectal cancer progression., Am J Transl Res, 8, 842

Wang, 2019, Downregulation of miR-222-3p reverses doxorubicin-resistance in LoVo cells through upregulating forkhead box protein P2 (FOXP2) protein., Med Sci Monit, 25, 2169, 10.12659/MSM.913325

Meng, 2018, Identification of key microRNAs associated with diffuse large B-cell lymphoma by analyzing serum microRNA expressions., Gene, 642, 205, 10.1016/j.gene.2017.11.022

Cong, 2018, Peroxiredoxin1 promotes cell proliferation, migration and invasion of colorectal cancer via p38MAPK signaling., Eur Rev Med Pharmacol Sci, 22, 1922

Deng, 2020, Prognostic and predictive role of DNA mismatch repair status in stage II-III colorectal cancer: a systematic review and meta-analysis., Clin Genet, 97, 25, 10.1111/cge.13628

Ding, 2019, Exosomes derived from human umbilical cord mesenchymal stromal cells deliver exogenous miR-145-5p to inhibit pancreatic ductal adenocarcinoma progression., Cancer Lett, 442, 351, 10.1016/j.canlet.2018.10.039

Yuan, 2019, Exosomes derived from microRNA-148b-3p-overexpressing human umbilical cord mesenchymal stem cells restrain breast cancer progression., Front Oncol, 9, 1076, 10.3389/fonc.2019.01076

Dong, 2019, Extracellular vesicles from human umbilical cord mesenchymal stem cells treated with siRNA against ELFN1-AS1 suppress colon adenocarcinoma proliferation and migration., Am J Transl Res, 11, 6989

Pakravan, 2017, MicroRNA-100 shuttled by mesenchymal stem cell-derived exosomes suppresses in vitro angiogenesis through modulating the mTOR/HIF-1α/VEGF signaling axis in breast cancer cells., Cell Oncol (Dordr), 40, 457, 10.1007/s13402-017-0335-7

Huang, 2019, ATG3, a target of miR-431-5p, promotes proliferation and invasion of colon cancer via promoting autophagy., Cancer Manag Res, 11, 10275, 10.2147/CMAR.S226828

Su, 2018, miR-431 inhibits colorectal cancer cell invasion via repressing CUL4B., Eur Rev Med Pharmacol Sci, 22, 3047

Wu, 2020, A novel three-miRNA signature identified using bioinformatics predicts survival in esophageal carcinoma., Biomed Res Int, 2020, 5973082

Pan, 2015, Correlation between down-expression of miR-431 and clinicopathological significance in HCC tissues., Clin Transl Oncol, 17, 557, 10.1007/s12094-015-1278-y

Sun, 2020, B3GNT3, a direct target of miR-149-5p, promotes lung cancer development and indicates poor prognosis of lung cancer., Cancer Manag Res, 12, 2381, 10.2147/CMAR.S236565

Thông tin
Thông tin xuất bản

Mutagenesis

Tập 37 Số 2

164-171

Thông tin tác giả