The reduction of oxidative stress, anticoagulation of platelets, and inhibition of lipopolysaccharide by conjugated linoleic acid bonded on a polysulfone membrane

Polymers for Advanced Technologies - Tập 18 Số 4 - Trang 286-291 - 2007
Fu‐Chen Kung1, Jung‐Jhih Chang1, Ming‐Chien Yang1
1Department of Polymer Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan 106, ROC

Tóm tắt

AbstractConjugated linoleic acid (CLA) was covalently bonded onto the surface of a polysulfone (PSF) membrane via esterification. The surface was characterized with contact angle measurement and FT‐IR spectrometery. The capability of bonded CLA on the suppression of oxidative stress was evaluated. The level of reactive oxygen species (ROS) was measured by the chemiluminescence (CL) method to evaluate the oxidative stress. Furthermore, the removal of bacterial endotoxin (lipopolysaccharide, LPS) by CLA‐bonding PSF was measured with enzyme‐linked immunosorbent assay (ELISA). The reduction in the platelet adhesion on the PSF surface was evaluated using a SF‐3000 automated hematology analyzer. The result indicates that CLA‐bonding PSF can reduce the adhesion of platelets by 73%. The concentration of LPS can be reduced by CLA‐bonding PSF 1.6 times more than unmodified PSF membrane. In addition, the level of ROS against CLA‐bonding PSF membrane was 23% of that against unmodified PSF membrane. Therefore CLA‐bonding PSF membrane could offer protection for patients against oxidative stress and could also inhibit LPS for clinical applications. Copyright © 2007 John Wiley & Sons, Ltd.

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Tài liệu tham khảo

10.1006/excr.1999.4499

Nicolosi RJ, 1997, Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters, Artery, 22, 266

10.1093/carcin/20.6.1019

10.1017/S0007114500000581

10.1007/s11745-997-0109-x

West DB, 1998, Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse, Am. J. Physiol., 44, 667

10.1093/jn/130.5.1140

10.1093/jn/129.1.32

10.3109/08923970009026004

10.1007/s11745-998-0236-4

10.1016/S1388-1981(99)00055-4

MacIntyre DE, 1984, Inhibition of platelet function by cis‐unsaturated fatty acids, Blood, 63, 848, 10.1182/blood.V63.4.848.848

10.1016/0005-2760(96)00025-2

Lemke HD, 1994, Methods for the detection of endotoxins present during extracorporeal circulation, Nephrol. Dial. Transplant, 9, 90

10.1016/0167-5699(92)90009-V

10.3168/jds.S0022-0302(99)75358-0

Williams CS, 1996, Prostaglandin endoperoxide synthase: Why two isoforms?, Am. J. Physiol., 270, 393

10.1093/carcin/17.5.1045

Ha YL, 1990, Inhibition of benzo[a]‐pyrene‐induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid, Cancer Res., 50, 1097

Cantwell H, 1998, The effect of conjugated linoleic acid and glutathione peroxide in oxidatively challenged liver cells, Biochem. Soc., 26, 69, 10.1042/bst026s062

10.1021/jf020086a

10.1021/jf010172v

10.1046/j.1523-1755.1999.07131.x

10.1016/j.colsurfb.2005.11.019

10.1023/A:1021150120408

10.1002/pat.337

10.1002/(SICI)1097-0290(19960905)51:5<581::AID-BIT10>3.0.CO;2-L

10.1016/S0955-2863(98)00014-X

10.1007/BF02529735

10.1016/0028-3908(96)00002-0

Rees RC, 1992, The Macrophage: The Natural Immune System, 315

10.1016/j.plipres.2004.08.002

10.1054/plef.2002.0454

10.1159/000059995

10.1159/000190247

10.1046/j.1523-1755.1998.00021.x

10.1046/j.1523-1755.1999.07131.x

10.1161/01.CIR.101.9.1002

10.1016/0891-5849(95)00078-C

10.1111/j.1349-7006.1997.tb00372.x

10.1159/000060004

Baggio B, 2005, Polyunsaturated fatty acids and renal fibrosis: Pathophysiologic link and potential clinical implications, J. Nephrol., 18, 362

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Polymers for Advanced Technologies

Tập 18 Số 4

286-291

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