Sản xuất subprotoplasts của thực vật bậc cao

Plant Molecular Biology Reporter - Tập 1 - Trang 117-123 - 1983
Peter M. Bradley1
1Dep't. of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester

Tóm tắt

Có nhiều ví dụ trong tài liệu cho thấy việc hình thành subprotoplasts được ưa chuộng trong điều kiện nồng độ osmotic cao và sự hiện diện của các tế bào kéo dài. Khi mô thực vật bị plasmolyze, một số tế bào kéo dài sẽ phân mảnh thành hai hoặc nhiều phần. Khi màng tế bào được loại bỏ bằng enzyme, các subprotoplasts sẽ được giải phóng vào môi trường. Nếu không có tế bào kéo dài, có thể sử dụng ly tâm hoặc sự nảy chồi của protoplasts, hoặc có thể thu được các tế bào lớn, dễ vỡ bằng cách xử lý với auxin ở nồng độ cao. Mỗi phương pháp tạo ra quần thể hỗn hợp của subprotoplasts và protoplasts. Các mẫu tinh khiết được thu nhận qua ly tâm gradient mật độ hoặc bằng cách chọn lọc bằng micropipet.

Từ khóa

#subprotoplasts #protoplasts #osmoticum #plasmolysed #enzyme #auxin #ly tâm gradient mật độ

Tài liệu tham khảo

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