The population of a high-virulence strain of Salmonella enterica serovar Enteritidis in subcutaneously infected partridge: a quantitative time-course study using real-time PCR
Tóm tắt
This research was undertaken to determine the population of a high-virulence strain of Salmonella enterica serovar Enteritidis in partridge by a fluorescent quencher PCR assay and to correlate these findings with the results obtained from the immunohistochemical localization and histopathological examinations of selected Salmonella enterica serovar Enteritidis-infected tissues. To make the results meaningful, a side-by-side bacteriology method (indirect immuno-fluorescent antibody staining) was performed too. The results of indirect immuno-fluorescent antibody staining and immunohistochemical localization were similar to the fluorescent quencher PCR assay. The time course of the appearance of bacterial antigens and tissue lesions in various tissues was coincident with the levels of the bacterial DNA loads at the infection sites. This suggests that Salmonella enterica serovar Enteritidis loads in internal organs are closely correlated with the progression of the infection.
Tài liệu tham khảo
Abshire KZ, Neidhardt FC (1993) Analysis of proteins synthesized by Salmonella Typhimurium during growth within a host macrophage. J Bacteriol 175:3734–3743
Agron PG, Walker RL, Kinde H, Sawyer SJ, Hayes DC, Wollard J, Andersen GL (2001) Identification by subtractive hybridization of sequences specific for Salmonella enterica serovar Enteritidis. Appl Environ Microbiol 67:4984–4991
Akaki C, Shimazu B, Baba T, Tsuji S, Kodama H, Mukamoto M, Kajikawa T (1997) Possible migration of Harderian gland immunoglobulin a bearing lymphocytes into the cecal tonsil in chickens. Zentralbl Veterinärmed B 44:199–206
Cirillo DM, Valdivia RH, Monack DM, Falkow S (1998) Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival. Mol Microbiol 30:175–188
Cheng ZQ, Shi YF, Zhang L, Zhu G, Diao XG, Cui ZZ (2007) Occurrence of reticuloendotheliosis in Chinese partridge. J Vet Med Sci 69:1295–1298
Deng SX, Cheng AC, Wang MS, Cao P (2007) Gastrotestinal tract distribution of Salmonella Enteritidis in orally infected mice with a species-specific fluorescent quantitative polymerase chain reaction. World J Gastroenterol 13:6568–6574
Deng SX, Cheng AC, Wang MS, Cao P (2008) Serovar-specific real-time quantitative detection of Salmonella Enteritidis in the gastrointestinal tract of ducks after oral challenge. Avian Dis 52:88–93
Dibb-Fuller MP, Allen-Vercoe E, Thorns CJ, Woodward MJ (1999) Fimbriae-and flagella-mediated association with and invasion of cultured epithelial cells by Salmonella Enteritidis. Microbiology 145:1023–1031
Dunlap NE, Beniamin WH, McCall RD, Tilden AB, Briles DE (1991) A safe site for Salmonella Typhimurium is within splenic cells during the early phase of infection in mice. Microb Pathog 10:297–310
Gast RK, Beard CW (1990) Serological detection of experimental Salmonella Enteritidis infections in laying hens. Avian Dis 34:721–728
Gillespie IA, Brien SJ, Adak GK, Ward LR, Smith HR (2005) Foodborne general outbreaks of Salmonella Enteritidis phage type 4 infection, Englang and Wales, 1992–2002: where are the risks? Epidemiol Infect 133:795–801
He GZ, Tian WY, Qian N, Cheng AC (2010) Quantitative studies of the distribution pattern for Salmonella Enteritidis in the internal organs of chicken after oral challenge by a real-time PCR. Vet Res Commun 34:669–676
Hope BK, Baker R, Edel ED, Hogue AT, Schlosser WD, Whiting R, McDowell RM, Morales RA (2002) An overview of the Salmonella Enteritidis risk assessment for shell eggs and egg products. Risk Anal 22:203–218
Holt PS, Porter R (1992) Microbiological and histopathological effects of an induced-molt fasting procedure on a Salmonella Enteritidis infection in chickens. Avian Dis 36:610–618
Islam MR, Nessa J, Halder KM (1993) Detection of duck plague virus antigen in tissues by immunoperoxidase staining. Avian Pathol 22:389–393
Lai CW, Chan RC, Cheng AF, Sung JY, Leung JW (1992) Common bile duct stones: a cause of chronic salmonellosis. Am J Gastroenterol 87:1198–1199
Massi M, Ioan P, Budriesi R, Chiarini A, Vitali B, Lammers KM, Gionchetti P, Campieri M, Lembo A, Briqidi P (2006) Effects of probiotic bacteria on gastrointestinal motility in guinea-pig isolated tissue. World J Gastroenterol 12:5987–5994
Mutinelli F, Capua I, Terregino C, Cattoli G (2003) Clinical, gross and microscopic findings in different avian species naturally infected during the H7N1 low-and high-pathogenicity avian influenza epidemics in Italy during 1999 and 2000. Avian Dis 47:844–848
Okamura M, Lillehoj HS, Raybourne RB, Babu US, Heckert RA, Tani H, Sasai K, Baba E, Lillehoj EP (2005) Differential responses of macrophages to Salmonella enterica serovars Enteritidis and Typhimurium. Vet Immunol Immunpathol 107:327–335
Xu C, Li ZS, Du YQ, Gong YF, Yang H, Sun B, Jin J (2007) Construction of recombinant attenuated Salmonella Typhimurium DNA vaccine expressing H pylori ure B and IL-2. World J Gastroenterol 13:939–944
Takata T, Liang J, Nakano H, Yoshimura Y (2003) Invasion of Salmonella Enteritidis in the tissues of reproductive organs in laying Japanese quail: an immunocytochemical study. Poult Sci 82:1170–1173
Yan B, Cheng AC, Wang MS, Deng SX, Zhang ZH, Yin NC, Cao P, Cao SY (2008) Application of indirect immunofluorescent staining method for detection Salmonella Enteritidis in paraffin slices and antigen location in infected duck tissues. World J Gastroenterol 7:776–781