Nghiên cứu về dinh dưỡng gen của chuột C57BL/6N được cho ăn chế độ ăn nhiều chất béo trong thời gian ngắn nhấn mạnh sự thay đổi sớm trong biểu hiện của các gen đồng hồ

Genes and Nutrition - Tập 8 - Trang 465-474 - 2013
Michela Lizier1,2, Lorenzo Bomba3, Andrea Minuti3,4, Fatima Chegdani4, Jessica Capraro5,6, Barbara Tondelli2,7, Raffaele Mazza3,8, Maria Luisa Callegari1, Erminio Trevisi3, Filippo Rossi5, Paolo Ajmone Marsan3,4, Franco Lucchini1,4
1Centro Ricerche Biotecnologiche, Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Cremona, Italy
2Istituto Clinico Humanitas IRCCS, Rozzano, Italy
3Istituto di Zootecnica, Università Cattolica del Sacro Cuore, Piacenza, Italy
4Centro Di Ricerca Sulla Nutrigenomica (NUTRIGEN), Università Cattolica del Sacro Cuore, Piacenza, Italy
5Istituto di Scienze degli Alimenti e della Nutrizione, Università Cattolica del Sacro Cuore, Piacenza, Italy
6Dipartimento di Scienze Molecolari Agroalimentari, Università degli Studi di Milano, Milan, Italy
7Istituto di Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Segrate, Italy
8Associazione Italiana Allevatori, Cremona, Italy

Tóm tắt

Chuột được cho ăn chế độ ăn nhiều chất béo lâu dài (HFD) là một mô hình đã được thiết lập cho các rối loạn chuyển hóa ở người, chẳng hạn như béo phì và tiểu đường. Tuy nhiên, cũng cần phải nghiên cứu những ảnh hưởng của việc cho ăn HFD trong thời gian ngắn để hiểu những sự kiện nào xảy ra đầu tiên kích hoạt sự khởi phát của tình trạng tiền bệnh, được gọi là hội chứng chuyển hóa, làm tăng nguy cơ phát triển các bệnh lâm sàng. Trong nghiên cứu này, chuột C57BL/6N được cho ăn chế độ ăn kiểm soát (CTR) hoặc HFD trong 1 tuần (T1) hoặc 2 tuần (T2). Các hiệu ứng chuyển hóa và mô học đã được kiểm tra. Transcriptome ruột già của chuột HFD và CTR đã được so sánh ở T2 bằng phân tích vi mạch. Các gen biểu hiện khác biệt đã được xác thực bằng PCR theo thời gian thực trong ruột già và trong gan. Sau 2 tuần cho ăn chế độ ăn, chuột HFD cho thấy một mô hình biểu hiện khác thường chỉ trong bảy gen, bốn trong số đó liên quan đến con đường điều chỉnh đồng hồ sinh học. PCR theo thời gian thực xác nhận kết quả vi mạch ở ruột già và tiết lộ xu hướng tương tự về sự thay đổi biểu hiện của các gen đồng hồ ở gan. Các phát hiện này gợi ý rằng các gen đồng hồ có thể đóng vai trò quan trọng trong việc kiểm soát sớm các hệ thống dẫn ra khỏi ruột để đáp ứng với HFD ở chuột và rằng sự thay đổi biểu hiện của chúng cũng có thể đại diện cho một tín hiệu sớm của sự phát triển tình trạng viêm ruột.

Từ khóa

#chuột C57BL/6N #chế độ ăn nhiều chất béo #gen đồng hồ #hội chứng chuyển hóa #biểu hiện gen #vi mạch

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