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Sự đa dạng phân tử của các mẫu khác nhau của Beauveria bassiana (Bals.) Vuill. được đánh giá bằng cách sử dụng các vi vệ tinh liên kết (ISSRs)
Tóm tắt
Các dấu hiệu PCR giữa vi vệ tinh (ISSR-PCR) đã được sử dụng để xác định và xem xét sự đa dạng di truyền của mười một dòng Beauveria bassiana với các nguồn gốc địa lý khác nhau. Đặc điểm biến đổi và mối quan hệ phát sinh loài giữa mười một chủng đã được phân tích bằng cách sử dụng 172 dấu hiệu ISSR-PCR. Một mức độ đa hình cao (gần 80%) đã được phát hiện khi sử dụng các dấu hiệu phân tử này. Bảy mẫu khác nhau cho thấy các băng tách biệt, và mồi ISSR 873 có khả năng phân biệt tất cả các chủng. Dendrogram thu được từ các dấu hiệu này là vững chắc và phù hợp với các nguồn gốc địa lý của các chủng này. Tất cả các mẫu từ khu vực Caribê được nhóm lại trong một cụm, trong khi các mẫu khác nhóm lại trong cụm khác. Độ tương đồng giữa hai cụm này nhỏ hơn 50%. Giá trị đồng loại này cho thấy sự đa dạng di truyền cao được phát hiện giữa các mẫu từ khu vực Caribê và các mẫu khác. Các dấu hiệu ISSR-PCR cung cấp một hệ thống nhanh chóng, đáng tin cậy và có thông tin cao cho việc nhận diện DNA, và cho phép xác định các dòng B. bassiana khác nhau đã được nghiên cứu.
Từ khóa
#Beauveria bassiana #ISSR-PCR #đa dạng di truyền #mối quan hệ phát sinh loàiTài liệu tham khảo
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