The microarray analysis for gene expression in haploids and diploids derived from twin-seedling rice

Science in China Series C: Life Sciences - Tập 51 - Trang 503-512 - 2008
HongYu Zhang1,2, Hai Peng3, PingChuan Li1, QiMing Deng1, PeiZhou Xu1, Yun Li4, XuDong Wang1, XianJun Wu1,2
1Rice Research Institute of Sichuan Agricultural University, Chengdu, China
2Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an, China
3Laboratory of Germplasm and Genetics, College of Life Science, Jianghan University, Wuhan, China
4Crop Research Institute of Agricultural and Forestal Science Academy of Chengdu, Chengdu, China

Tóm tắt

In this study, microarray technique was employed to analyze the gene expression at the RNA level between haploids and corresponding diploids derived from a rice twin-seedling line SARII-628. Different degrees of expression variations were observed in the plant after haploidization. The main results are as follows: (1) after haploidization, the ratio of the sensitive loci was 2.47% of the total loci designed on chip. Those loci were randomly distributed on the 12 pairs of rice chromosomes and the activated loci were more than the silenced ones. (2) Gene clusters on chromosome were observed for 33 sequences. (3) GoPipe function classification for 575 sensitive loci revealed an involvement in the biological process, cell component and molecular function. (4) RT-PCR generally validated the result from microarray with a coincidence rate of 83.78%. And for the randomly-selected activated or silenced loci in chip analysis, the coincidence rate was up to 91.86%.

Tài liệu tham khảo

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Science in China Series C: Life Sciences

Tập 51

503-512

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