The membrane bioreactor: A novel tool to grow anammox bacteria as free cells

Biotechnology and Bioengineering - Tập 101 Số 2 - Trang 286-294 - 2008
Wouter R. L. van der Star1,2,3, Andreea I. Miclea1,4,2,3, Udo G.J.M. van Dongen1,2,3, Gerard Muyzer1,2,3, Cristian Picioreanu1,2,3, Mark C.M. van Loosdrecht1,2,3
1Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628BC, Delft, The Netherlands
2fax: +31-15-2782355
3telephone: +31-15-2781618
4Department of Experimental Biology, Faculty of Biology and Geology, Babeş-Bolyai University, Clinicolor Street 5-7, Cluj-Napoca, Romania

Tóm tắt

AbstractIn a membrane bioreactor (MBR), fast growth of anammox bacteria was achieved with a sludge residence time (SRT) of 12 days. This relatively short SRT resulted in a—for anammox bacteria—unprecedented purity of the enrichment of 97.6%. The absence of a selective pressure for settling, and dedicated cultivation conditions led to growth in suspension as free cells and the complete absence of flocs or granules. Fast growth, low levels of calcium and magnesium, and possibly the presence of yeast extract and a low shear stress are critical for the obtainment of a completely suspended culture consisting of free anammox cells. During cultivation, a population shift was observed from Candidatus “Brocadia” to Candidatus “Kuenenia stuttgartiensis.” It is hypothesized that the reason for this shift is the higher affinity for nitrite of “Kuenenia.” The production of anammox bacteria in suspension with high purity and productivity makes the MBR a promising tool for the cultivation and study of anammox bacteria. Biotechnol. Bioeng. 2008;101: 286–294. © 2008 Wiley Periodicals, Inc.

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Thông tin xuất bản

Biotechnology and Bioengineering

Tập 101 Số 2

286-294

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