The melanin‐concentrating hormone system of the rat brain: An immuno‐ and hybridization histochemical characterization

Journal of Comparative Neurology - Tập 319 Số 2 - Trang 218-245 - 1992
Jackson C. Bittencourt1, Françoise Presse2, Carlos Arias1, Charles A. Peto1, Joan Vaughan2, Jean‐Louis Nahon2, Wylie Vale2, Paul E. Sawchenko1
1Laboratory of Neuronal Structure and Function, The Salk Institute for Biological Studies, La Jolla, California 92037
2The Clayton Foundation Laboratories of Peptide Biology, The Salk Institute for Biological Studies, La Jolla, California 92037

Tóm tắt

AbstractIn addition to a nonadecapeptide homologous to the teleost melanin‐concentrating hormone (MCH), the amino acid sequence predicted from a rat prepro‐MCH (ppMCH) cDNA suggested that at least one (neuropeptide EI, or NEI), and possibly a second (NGE), additional neuropeptide may be encoded by this precursor. Cross‐reactivity with epitopes of NEI or NGE can account for reported localization of α‐MSH, rat CRF, and human GRF in rat dorsolateral hypothalamic neurons. We have used antisera raised against rat MCH and NEI in immunohistochemical studies at the light and electron microscopic levels, along with hybridization histochemical localization of ppMCH mRNA, to define the organization of this system. As expected, ppMCH mRNA is prominently expressed in cells in the lateral hypothalamic area and zona incerta. The MCH and NEI peptides were extensively colocalized in neurons in both of these areas. In addition, smaller cell groups in the olfactory tubercle and pontine tegmentum were also positively hybridized for ppMCH mRNA and immunostained for MCH and NEI. Fibers stained for MCH and NEI were similarly, and very broadly, distributed throughout the central nervous system in patterns that generally conformed with known projection fields of the lateral hypothalamic area and zona incerta. A differential distribution was seen in at least one region, the interanterodorsal nucleus of the thalamus, which contained a prominent terminal field stained for MCH but not NEI. At the electron microscopic level, MCH‐stained perikarya displayed a prominent staining associated with the Golgi apparatus; this was not encountered in NEI‐stained cells. Both peptides were distributed similarly in terminals in the lateral hypothalamic area and median eminence, with staining associated principally with dense‐cored vesicles. The results suggest that ppMCH‐derived peptides may serve as neurotransmitters or modulators of prominence in a surprisingly expansive projection field of incerto‐hypothalamic neurons. The terminal distributions of this system seem most compatible with functional roles in generalized arousal and sensorimotor integration, processes previously implicated as being subject to modulation by the lateral hypothalamic area. © 1992 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1002/ar.1090940210

Baker B. I., 1988, The Melanotropic Peptides, 160

10.1016/S0074-7696(08)60681-6

10.1677/joe.0.106R005

10.1016/0361-9230(82)90009-0

Bittencourt J. C., 1990, Distribution of prepro‐melanin‐concentrating hormone‐derived peptides and mRNA in the rat brain, Soc. Neurosci. Abstr., 16, 361

Bleier R., 1979, Anatomy of the Hypothalamus Handbook of the Hypothalamus, Vol, 137

Brownstein M. J., 1986, Coexistence of Neuronal Messengers: A New Principle in Chemical Transmission. Prog. Brain Res., 161

Eberle A. N., 1988, The Melanotropins, 321

10.1016/0306-4522(81)90065-8

10.1002/j.1460-2075.1982.tb01175.x

10.1146/annurev.cb.01.110185.002311

10.1016/0304-3940(89)90196-1

10.1016/0304-3940(85)90334-9

10.1016/0304-3940(87)90309-0

10.1016/0304-3940(86)90241-7

Gallistel C. R., 1985, Forebrain origins and terminations of the medial forebrain bundle metabolically activated by rewarding stimulation or by reward‐blocking doses of Pimozide, J. Neurosci., 5, 1246, 10.1523/JNEUROSCI.05-05-01246.1985

10.1016/0006-8993(84)90940-5

10.1002/cne.900430102

10.1016/0006-8993(80)90236-X

10.1016/0304-3940(81)90232-9

Hosoya Y., 1983, Structure and Function of Peptidergic and Aminergic Neurons. Taniguchi Symposia on Brain Sciences No. 5, 13

10.1002/cne.902720208

10.1038/305321a0

10.1016/0143-4179(86)90023-5

Khachaturian H., 1985, Handbook of Chemical Neuroanatomy, Vol. 4: GABA and Neuropeptides in the CNS, Part I, 216

10.1002/cne.902230404

10.1016/0306-4522(85)90049-1

Lorente de Nó R., 1943, Physiology of the Nervous System, 274

10.1016/0301-0082(87)90004-9

10.1016/0006-8993(88)90012-1

10.1002/cne.902280409

10.1210/endo-125-4-2056

10.1111/j.1365-2826.1991.tb00260.x

Nahon J.‐L., 1989, Recent Advances in Basic and Clinical Neuroendocrinology, 15

10.1007/BF00222284

10.1016/0304-3940(86)90441-6

10.1677/joe.0.127R001

10.1002/cne.902060106

Page R. B., 1986, Current Topics in Neuroendocrinology, Vol. 7: Morphology of Hypothalamus and Its Connections, 1

Parkes D. G., 1991, Secretion of melanin concentrating hormone from cultured rat hypothalamic cells, Soc. Neurosci. Abstr., 17, 1193

10.1016/0006-8993(87)90846-8

Pickel V. M., 1985, Handbook of Chemical Neuroanatomy, 72

10.1177/34.6.2422251

10.1523/JNEUROSCI.09-04-01375.1989

10.1016/0006-8993(81)90437-6

10.1016/0006-8993(82)90493-0

10.1002/cne.902220302

10.1002/cne.902370103

10.1016/0361-9230(86)90018-3

Saper C. B., 1979, An autoradiographic study of the efferent connections of the lateral hypothalamic area in the rat., 183, 689

10.1002/cne.902910209

10.1016/B978-0-12-185255-9.50018-3

10.1002/cne.902410203

10.1523/JNEUROSCI.04-04-01118.1984

10.1016/0304-3940(88)90346-1

Simmons D. M., 1989, A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radiolabeled single‐stranded RNA probes, J. Histotechnol., 12, 169, 10.1179/014788889794651870

10.1016/0361-9230(85)90213-8

10.1016/0306-4522(87)90077-7

Stricker E. M., 1976, Recovery of function after damage to central catecholamine‐containing neurons: A neurochemical model for the lateral hypothalamic syndrome, Prog. Psychobiol. Physiol. Psychol., 6, 121

Swanson L. W., 1987, Handbook of Chemical Neuroanatomy, Vol. 5: Integrated Systems of the CNS, Part I, 1

10.1073/pnas.78.11.6603

10.1177/26.4.207771

Vale W., 1936, Methods in Enzymology: Hormone Action: Neuroendocrine Peptides, 389

Valenstein E. S., 1969, Reinforcement and Behavior, 242, 10.1016/B978-0-12-683650-9.50014-5

10.1007/BF00232234

10.1210/endo-125-3-1660

10.1002/cne.902060107

10.1016/0306-4522(87)90351-4

10.1016/0304-3940(87)90345-4

10.1016/0304-3940(87)90346-6

10.1002/cne.902840212

10.1146/annurev.cb.02.110186.002435

10.1016/0014-2999(79)90351-0

10.1016/0006-8993(80)90849-5

10.1007/BF00300012

Zamboni L., 1967, Buffered picric acid‐formaldehyde: A new, rapid fixative for electron microscopy, J. Cell Biol., 35, 148A

10.1073/pnas.83.5.1528

Thông tin
Thông tin xuất bản

Journal of Comparative Neurology

Tập 319 Số 2

218-245

Thông tin tác giả