The measurement of average hydrodynamic velocity in capillary zone electrophoresis

Springer Science and Business Media LLC - Tập 41 - Trang 343-348 - 2014
H. -W. Zhang1, X. L. Kou1, Z. D. Hu1
1Department of Chemistry, Lanzhou University, Lanzhou, P.R. China

Tóm tắt

Based on the linear relationship between large sample injection time during hydrodynamic injection and migration time in non-stacking runs, a new method is proposed to measure average hydrodynamic velocity in capillary zone electrophoresis (CZE). Only the migration times for large injections need to be measured in this new approach, so the method is simple. Additionally, a modified Poiseuille equation is proposed to eliminate the deviation involved in Poiseuille’s equation. The average hydrodynamic velocity values determined by our method generally compare favorably with those determined by the modified Poiseuille equation.

Tài liệu tham khảo

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