The kinetic activity of green synthesized CuO NPs using Eucalyptus globulus leaf extract and its synergistic effect with antibiotics on MDR Shigella sonnei
Biologia - Trang 1-10 - 2023
Tóm tắt
This study investigated the antimicrobial activity of the green synthesis copper oxide nanoparticles (CuO-NPs) from Eucalyptus globulus leaf extract against S. sonnei clinical isolates and the reference strain S. sonnei ATCC 9290 alone and in synergy with antibiotics. Biosynthesized NPs analyzed for shape, size, and crystal nature. The antimicrobial genotype and phenotype characteristic for selecting the most resistant isolates from 30 S. sonnei clinical isolates performed through the distribution of ipaH, sigA, and virF, CTX-M-1, CIT group, aac(6’)-Ib-cr genes by polymerase chain reaction (PCR) technology and Kirby-Bauer susceptibility test. The minimum inhibitory concentration (MIC) of CuO-NPs against S. sonnei was determined within 0–360 min treatment time. The double-disc method was performed for semi-sensitive and antibiotic-resistant strains to evaluate the probable inhibitory effect in synergy form. XRD, TEM, and FTIR analysis confirmed the successful synthesis of NPs with appropriate purity, shape, and size. Only one of the S. sonnei isolates was positive for all MDR-related genes (blaCTX-M-1 and blaCIT, aac(6’)-Ib-cr) and showed the highest susceptibility reaction against CuO-NPs, Z = 312.5 mL.µg−1 in comparison to Z = 256.25 mL.µg−1 for the standard strain, and the process continued by performing the optimal ratio of NPs on semi-sensitive and also resistance antibiotic in synergy with NPs for the bacteria isolated. Furthermore, the synergy effect of CuO-NPs with oxacillin, chloramphenicol, imipenem, and levofloxacin was higher than using antibiotics alone. CuO-NPs, enhance the properties and characteristics of antibacterial potency in synergy or developed synthetic functionalized NPs with antibiotics.
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