Thesldmutation is specific for sublingual salivary mucous cells and disrupts apomucin gene expression
Tóm tắt
NFS/N- sld mice harbor a spontaneous autosomal recessive mutation, sld (sublingual gland differentiation arrest) and histologically display attenuated mucous cell expression in sublingual glands (Hayashi et al. Am J Pathol 132: 187–191, 1988). Because altered serous demilune cell expression is unknown, we determined the phenotypic expression of this cell type in mutants. Moreover, we evaluated whether absence of glycoconjugate staining in 3-day-old mutant glands is related to disruption in apomucin gene expression and/or to posttranslational glycosylation events. Serous cell differentiation is unaffected, determined morphologically and by serous cell marker expression (PSP, parotid secretory protein; and Dcpp, demilune cell and parotid protein). Conversely, apical granules in “atypical” exocrine cells of mutant glands are PSP and mucin negative, but contain abundant SMGD (mucous granule marker). Age-related appearance of mucous cells is associated with expression of apomucin gene products, whereas SMGD expression is unaltered. “Atypical” cells thus appear specified to a mucous cell fate but do not synthesize mucin glycoproteins unless selectively induced postnatally, indicating the sld mutation disrupts apomucin transcriptional regulation and/or decreases apomucin mRNA stability.
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Tài liệu tham khảo
Denny PA and Denny PC.Partial sequence of a large transcript from mouse sublingual glands.J Dent Res76: 158, 1997.
Girard LR, Castle AM, Hand AR, Castle JD, and Mirels L.Characterization of common salivary protein 1, a product of rat submandibular, sublingual, and parotid glands.J Biol Chem268: 26592–26601, 1993.
Hayashi Y, Kojima A, Hata M, and Hirokawa K.A new mutation involving the sublingual gland in NFS/N mice. Partially arrested mucous cell differentiation.Am J Pathol132: 187–191, 1988.
Kojima A and Hata M.Sublingual gland differentiation arrest in the NFS/N subline.Mouse News Lett80: 147, 1988.