Thesldmutation is specific for sublingual salivary mucous cells and disrupts apomucin gene expression

Physiological Genomics - Tập 14 Số 2 - Trang 95-106 - 2003
Monica Fallon1, Lisa R. Latchney1, Arthur R. Hand2, Angad Johar3, Patricia A. Denny4, Philippe Georgel1, Paul Denny4, David J. Culp1
1University of Rochester Medical Center, Center for Oral Biology and the Department of Pharmacology and Physiology, Rochester, New York 14642
2Departments of Pediatric Dentistry
3BioStructure and Function, University of Connecticut Health Center, School of Dental Medicine, Farmington, Connecticut 06030
4University of Southern California, Division of Diagnostic Sciences, School of Dentistry, Los Angeles, California 90089

Tóm tắt

NFS/N- sld mice harbor a spontaneous autosomal recessive mutation, sld (sublingual gland differentiation arrest) and histologically display attenuated mucous cell expression in sublingual glands (Hayashi et al. Am J Pathol 132: 187–191, 1988). Because altered serous demilune cell expression is unknown, we determined the phenotypic expression of this cell type in mutants. Moreover, we evaluated whether absence of glycoconjugate staining in 3-day-old mutant glands is related to disruption in apomucin gene expression and/or to posttranslational glycosylation events. Serous cell differentiation is unaffected, determined morphologically and by serous cell marker expression (PSP, parotid secretory protein; and Dcpp, demilune cell and parotid protein). Conversely, apical granules in “atypical” exocrine cells of mutant glands are PSP and mucin negative, but contain abundant SMGD (mucous granule marker). Age-related appearance of mucous cells is associated with expression of apomucin gene products, whereas SMGD expression is unaltered. “Atypical” cells thus appear specified to a mucous cell fate but do not synthesize mucin glycoproteins unless selectively induced postnatally, indicating the sld mutation disrupts apomucin transcriptional regulation and/or decreases apomucin mRNA stability.

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Physiological Genomics

Tập 14 Số 2

95-106

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