The <i>Pristionchus</i> HOX gene <i>Ppa-lin-39</i> inhibits programmed cell death to specify the vulva equivalence group and is not required during vulval induction

Development (Cambridge) - Tập 125 Số 19 - Trang 3865-3873 - 1998
Natsumi Kanzaki1, Andreas Eizinger1, Kwang‐Zin Lee1, Benno Jungblut1, Anja Bubeck1, Isabel Schlak1
1Max-Planck Institut für Entwicklungsbiologie, Abt. Zellbiologie, Spemannstrasse 35, 72076 Tübingen, Germany

Tóm tắt

ABSTRACT In the two nematode species Caenorhabditis elegans and Pristionchus pacificus the vulva equivalence group in the central body region is specified by the Hox gene lin-39. C. elegans lin-39 mutants are vulvaless and the vulval precursor cells fuse with the surrounding hypodermis, whereas in P. pacificus lin-39 mutants the vulval precursor cells die by apoptosis. Mechanistically, LIN-39 might inhibit non-vulval fate (cell fusion in C. elegans, apoptosis in P. pacificus), promote vulval fate or do both. To study the mechanism of lin-39 function, we isolated P. pacificus cell death mutants and identified mutations in ced-3. Surprisingly, P. pacificus ced-3; lin-39 double mutants form a functional vulva in the absence of LIN-39 activity. Thus, in P. pacificus lin-39 specifies the vulva equivalence group by inhibiting programmed cell death. Furthermore, these data reveal an important difference in a later function of lin-39 between the two species. In C. elegans, LIN-39 specifies vulval cell fates in response to inductive RAS signaling, and in P. pacificus LIN-39 is not required for vulval induction. Thus, the comparative analysis indicates that lin-39 has distinct functions in both species although the gene is acting in a homologous developmental system.

Từ khóa


Tài liệu tham khảo

Aroian, 1990, The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor subfamily, Nature, 348, 693, 10.1038/348693a0

Beitel, 1990, Caenorhabditis elegans ras gene let-60 acts as a switch in the pathway of vulva induction, Nature, 348, 503, 10.1038/348503a0

Beitel, 1995, The Caenorhabditis elegans gene lin-1 encodes an ETS domain protein and defines a branch of the vulva induction pathway, Genes Dev, 9, 3149, 10.1101/gad.9.24.3149

Cerretti, 1992, Molecular cloning of the interleukin-1β converting enzyme, Science, 256, 97, 10.1126/science.1373520

Clandinin, 1997, Caenorhabditis elegans HOM-C genes regulate the response of vulval precursor cells to inductive signal. Dev. Biol, 182, 150

Clark, 1993, Control of cell fates in the central body region of C. elegans by the homeobox gene lin-39, Cell, 74, 43, 10.1016/0092-8674(93)90293-Y

Emmons, 1997, C. elegans II, 295

Eizinger, 1997, The homeotic gene lin-39 and the evolution and nematode epidermal cell fates, Science, 278, 452, 10.1126/science.278.5337.452

Epstein, 1995, Caenorhabditis elegans: Modern biological analysis of an organism

Félix, 1998, A gonad-derived survival signal for vulval precursor cells in two nematode species, Curr. Biol, 8, 287, 10.1016/S0960-9822(98)70111-3

Han, 1990, let-60, a gene that specifies cell fates during C. elegans vulval induction encodes a ras protein, Cell, 63, 921, 10.1016/0092-8674(90)90495-Z

Hengartner, 1997, C. elegans II, 295

Hill, 1992, The gene lin-3 encodes an inductive signal for vulval development in C. elegans, Nature, 358, 470, 10.1038/358470a0

Jungblut, 1998, The Pristionchus pacificus mab-5 gene is involved in the regulation of ventral epidermal cell fates, Curr. Biol, 8, 775, 10.1016/S0960-9822(98)70301-X

Kimble, 1981, Lineage alterations after ablation of cells in the somatic gonad of C. elegans, Dev. Biol, 87, 286, 10.1016/0012-1606(81)90152-4

Kornfeld, 1997, Vulval development in Caenorhabditis elegans, Trends Genet, 13, 55, 10.1016/S0168-9525(97)01005-6

Krumlauf, 1994, Hox genes in vertebrate development, Cell, 78, 191, 10.1016/0092-8674(94)90290-9

Lackner, 1994, A MAP kinase homolog, mpk-1, is involved in ras-mediated induction of vulval cell fates in Caenorhabditis elegans, Genes Dev, 8, 160, 10.1101/gad.8.2.160

Lawrence, 1994, Homeobox genes: their function in Drosophila segmentation and pattern formation Cell, 78, 181

Maloof, 1998, The HOX gene lin-39 is required during C. elegans vulval induction to select the outcome of Ras signaling, Development, 125, 181, 10.1242/dev.125.2.181

Raff, 1996, The shape of life, 10.7208/chicago/9780226256573.001.0001

Salser, 1994, Patterning C. elegans: homeotic cluster genes, cell fates and cell migrations, Trends Genet, 10, 159, 10.1016/0168-9525(94)90092-2

Sambrook, 1989, Molecular Cloning

Schlötterer, 1996, M13mtvh: an improved M13 vector for rapid and simple cloning of PCR products, Trends Genet, 12, 286, 10.1016/S0168-9525(96)90102-X

Sommer, 1996, Morphological, genetic and molecular description of Pristionchus pacificus sp. n. (Nematoda: Neodiplogastridae, Fund. Appl. Nemat, 19, 511

Sommer, 1996, Apoptosis and change of competence limit the size of the vulva equivalence group in Pristionchus pacificus: a genetic analysis, Curr. Biol, 6, 52, 10.1016/S0960-9822(02)00421-9

Sulston, 1977, Postembryonic cell lineages of the nematode Caenorhabditis elegans, Dev. Biol, 56, 110, 10.1016/0012-1606(77)90158-0

Sulston, 1980, The Caenorhabditis elegans male: Postembryonic development of nongonadal structures, Dev. Biol, 78, 542, 10.1016/0012-1606(80)90352-8

Thorberry, 1992, A novel heterodimeric cysteine protease is required for interleukin-1β processing in monocytes, Nature, 356, 768, 10.1038/356768a0

Walker, 1994, Crystal structure of the cysteine protease Interleukin-1β-converting enzyme: A (p20/p10)2 homodimer, Cell, 78, 343, 10.1016/0092-8674(94)90303-4

Wang, 1993, A homeotic gene cluster patterns the anteroposterior body axis of C. elegans, Cell, 74, 29, 10.1016/0092-8674(93)90292-X

Wilson, 1994, Structure and mechanism of interleukin-1β converting enzyme, Nature, 370, 270, 10.1038/370270a0

Wu, 1994, Suppression of activated let-60 ras protein defines a role of Caenorhabditis elegans Sur-1 MAP kinase in vulval differentiation, Genes Dev, 8, 147, 10.1101/gad.8.2.147

Xue, 1996, The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of human CPP32 protease, Genes Dev, 10, 1073, 10.1101/gad.10.9.1073

Yuan, 1993, The C. elegans cell death ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzyme, Cell, 75, 641, 10.1016/0092-8674(93)90485-9

Thông tin
Thông tin xuất bản

Development (Cambridge)

Tập 125 Số 19

3865-3873

Thông tin tác giả