The Arabidopsis EIN3 Binding F-Box Proteins EBF1 and EBF2 Have Distinct but Overlapping Roles in Ethylene Signaling

Plant Cell - Tập 19 Số 2 - Trang 509-523 - 2007
Brad M. Binder1, Joseph Walker2, Jennifer M. Gagne2, Thomas J. Emborg2, Georg Hemmann2, Anthony B. Bleecker3, Richard D. Vierstra2
1Department of Botany University of Wisconsin Madison, Wisconsin 53706 USA
2Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706
3Department of Botany, University of Wisconsin, Madison, Wisconsin 53706

Tóm tắt

Abstract

Ethylene signaling in Arabidopsis thaliana converges on the ETHYLENE-INSENSITIVE3 (EIN3)/EIN3-Like (EIL) transcription factors to induce various responses. EIN3 BINDING F-BOX1 (EBF1) and EBF2 were recently shown to function in ethylene perception by regulating EIN3/EIL turnover. In the absence of ethylene, EIN3 and possibly other EIL proteins are targeted for ubiquitination and subsequent degradation by Cullin 1–based E3 complexes containing EBF1 and 2. Ethylene appears to block this ubiquitination, allowing EIN3/EIL levels to rise and mediate ethylene signaling. Through analysis of mutant combinations affecting accumulation of EBF1, EBF2, EIN3, and EIL1, we show that EIN3 and EIL1 are the main targets of EBF1/2. Kinetic analyses of hypocotyl growth inhibition in response to ethylene and growth recovery after removal of the hormone revealed that EBF1 and 2 have temporally distinct but overlapping roles in modulating ethylene perception. Whereas EBF1 plays the main role in air and during the initial phase of signaling, EBF2 plays a more prominent role during the latter stages of the response and the resumption of growth following ethylene removal. Through their coordinated control of EIN3/EIL1 levels, EBF1 and EBF2 fine-tune ethylene responses by repressing signaling in the absence of the hormone, dampening signaling at high hormone concentrations, and promoting a more rapid recovery after ethylene levels dissipate.

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Tài liệu tham khảo

1999, Science, 284, 2148, 10.1126/science.284.5423.2148

2003, Science, 301, 653, 10.1126/science.1086391

2003, Proc. Natl. Acad. Sci. USA, 100, 2992, 10.1073/pnas.0438070100

1998, Theor. Appl. Genet., 97, 729, 10.1007/s001220050949

1976, Plant Physiol., 58, 268, 10.1104/pp.58.3.268

2004, Plant Physiol., 136, 2921, 10.1104/pp.104.050393

2004, Plant Physiol., 136, 2913, 10.1104/pp.104.050369

1997, Cell, 89, 1133, 10.1016/S0092-8674(00)80300-1

2005, Ann. Bot. (Lond.), 95, 901, 10.1093/aob/mci100

2005, Dev. Cell, 9, 109, 10.1016/j.devcel.2005.05.014

1999, Annu. Rev. Cell Dev. Biol., 15, 435, 10.1146/annurev.cellbio.15.1.435

2003, Plant J., 35, 729, 10.1046/j.1365-313X.2003.01844.x

2001, Plant J., 26, 471, 10.1046/j.1365-313x.2001.01038.x

2002, Proc. Natl. Acad. Sci. USA, 99, 11519, 10.1073/pnas.162339999

2004, Proc. Natl. Acad. Sci. USA, 101, 6803, 10.1073/pnas.0401698101

2005, J. Biol. Chem., 280, 18810, 10.1074/jbc.M413247200

2003, Cell, 115, 667, 10.1016/S0092-8674(03)00969-3

2004, Curr. Opin. Plant Biol., 7, 40, 10.1016/j.pbi.2003.11.011

1990, Plant Cell, 2, 513

1999, Plant Physiol., 119, 713, 10.1104/pp.119.2.713

1993, Cell, 12, 427

1996, Cell, 85, 183, 10.1016/S0092-8674(00)81095-8

2004, Dev. Cell, 7, 193, 10.1016/j.devcel.2004.07.002

2006, Proc. Natl. Acad. Sci. USA, 103, 13286, 10.1073/pnas.0605528103

2003, EMBO J., 22, 1282, 10.1093/emboj/cdg131

1996, Comput. Appl. Biosci., 12, 357

1999, Proc. Natl. Acad. Sci. USA, 96, 14142, 10.1073/pnas.96.24.14142

2003, Cell, 115, 679, 10.1016/S0092-8674(03)00968-1

2006, Plant Cell, 18, 3047, 10.1105/tpc.106.046508

1995, Genetics, 139, 1393, 10.1093/genetics/139.3.1393

2002

2002, Plant Cell, 14, 17, 10.1105/tpc.010381

2004, Annu. Rev. Plant Biol., 55, 555, 10.1146/annurev.arplant.55.031903.141801

1998, Genes Dev., 12, 3703, 10.1101/gad.12.23.3703

1997, Nucleic Acids Res., 24, 4876

1996, Plant Cell, 8, 735

2004, Plant J., 37, 505, 10.1046/j.1365-313X.2003.01975.x

2002, Plant Cell, 14, S131, 10.1105/tpc.001768

2003, Nature, 425, 521, 10.1038/nature01984

1984, Annu. Rev. Plant Physiol., 35, 155, 10.1146/annurev.pp.35.060184.001103

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Plant Cell

Tập 19 Số 2

509-523

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